Park7/DJ-1 Antibody

Novus Biologicals | Catalog # NB100-1194

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Park7/DJ-1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-AAQVKAPLVLKD corresponding to C-Terminus according to NP_009193.2.

Localization

Cytoplasmic and Nuclear.

Specificity

Variants (NP_001116849.1; NP_009193.2) encode the same protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Park7/DJ-1 Antibody

Western Blot: Park7/DJ-1 Antibody [NB100-1194]

Western Blot: Park7/DJ-1 Antibody [NB100-1194]

Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Park7/DJ-1 Antibody

Western Blot: Park7/DJ-1 Antibody [NB100-1194] -

Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
Park7/DJ-1 Antibody

Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody [NB100-1194] -

Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody [NB100-1194] - Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml NB100-1194 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.
Park7/DJ-1 Antibody

Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] -

Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] - (7ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Park7/DJ-1 Antibody

Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] -

Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.

Applications for Park7/DJ-1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry-Paraffin

7 ug/ml

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5-1 ug/ml
Application Notes
WB: Approx. 24 kDa band observed in HeLa and Jurkat lysates and of human brain (cerebellum, hippocampus, frontal cortex) and rodent brain lysates (calculated MW of 19.9 kDa band according to NP_001116849.1 and of NP_009193.2).

Reviewed Applications

Read 1 review rated 3 using NB100-1194 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Park7/DJ-1

PARK7/DJ1 is a ubiquitously expressed protein involved in various cellular processes including cell proliferation, RNA-binding, and oxidative stress. The protein has been found to colocalize within a subset of pathologic tau inclusions in a diverse group of neurodegenerative disorders known as tauopathies (Rizzu et al. 2004). Defects in PARK7/DJ1 are the cause of autosomal recessive early-onset Parkinson's disease 7 (PARK7). Parkinson's disease (PD) is a complex, multifactorial disorder that typically manifests after the age of 50 years. The disease is characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain. PARK7 is characterized by onset before 40 years and slow progression. It has also been suggested that PARK7/DJ1 is a mitogen dependent oncogene product involved in Ras related signal transduction pathways.

Long Name

Parkinson Disease 7

Alternate Names

DJ-1, DJ1

Entrez Gene IDs

11315 (Human); 57320 (Mouse); 117287 (Rat)

Gene Symbol

PARK7

UniProt

Q99497

Additional Park7/DJ-1 Products

Product Documents for Park7/DJ-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Park7/DJ-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Park7/DJ-1 Antibody

Customer Reviews for Park7/DJ-1 Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID:22836768
    Species: Human
    Verified Customer | Posted 12/12/2014

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Protocols

View specific protocols for Park7/DJ-1 Antibody (NB100-1194):

Western Blot Protocol for Park7(DJ-1) Antibody (NB100-1194):
Western blot Protocol:

1) Transfer onto PVDF membrane
2) Block in 3% (w/v) skimmed milk in TBS-T (0.05% Tween 20) for 1 hr at room temperature with agitation (can be blocked overnight at 4C without agitation).
3) Incubate NB100-1194 (2ug/ml) for 1 hr at room temperature with agitation.
4) Wash with TBS-T three times
5) Incubate with secondary antibody (1:20,000 or higher). We use Jackson or Rockland anti-goat secondary (affinity purified and with minimal cross-reactivity with Human and rodent serum proteins) for 1 hr at room temperature with agitation.
6) Wash with TBST three times.
7) Apply the detection reagent of choice in accordance with the manufacturers instructions (We use ECL-plus (Amersham) rather than ECL, which is more sensitive for detection.)

Notes:

*All antibodies are incubated in blocking buffer.
*QC testing was done under reducing conditions (with beta mercaptoethanol at 100C).

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FAQs

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