![Western Blot: Park7/DJ-1 Antibody [NB100-1194]](https://resources.rndsystems.com/images/products/Park7-DJ-1-Antibody-Western-Blot-NB100-1194-img0007.jpg "Western Blot: Park7/DJ-1 Antibody [NB100-1194]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-AAQVKAPLVLKD corresponding to C-Terminus according to NP_009193.2.
Localization
Cytoplasmic and Nuclear.
Specificity
Variants (NP_001116849.1; NP_009193.2) encode the same protein.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Theoretical MW
20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Park7/DJ-1 Antibody
Western Blot: Park7/DJ-1 Antibody [NB100-1194]
Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Park7/DJ-1 Antibody [NB100-1194] -
Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody [NB100-1194] -
Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody [NB100-1194] - Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml NB100-1194 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.
Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] -
Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] - (7ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] -
Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.Applications for Park7/DJ-1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5 ug/ml
Immunohistochemistry-Paraffin
7 ug/ml
Peptide ELISA
Detection limit 1:128000
Western Blot
0.5-1 ug/ml
Application Notes
WB: Approx. 24 kDa band observed in HeLa and Jurkat lysates and of human brain (cerebellum, hippocampus, frontal cortex) and rodent brain lysates (calculated MW of 19.9 kDa band according to NP_001116849.1 and of NP_009193.2).
Reviewed Applications
Read 1 review rated 3 using NB100-1194 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Park7/DJ-1
Long Name
Parkinson Disease 7
Alternate Names
DJ-1, DJ1
Gene Symbol
PARK7
UniProt
Additional Park7/DJ-1 Products
Product Documents for Park7/DJ-1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Park7/DJ-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Park7/DJ-1 Antibody
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Customer Reviews for Park7/DJ-1 Antibody (1)
3 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: See PMID:22836768Species: HumanVerified Customer | Posted 12/12/2014
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Protocols
View specific protocols for Park7/DJ-1 Antibody (NB100-1194):
Western Blot Protocol for Park7(DJ-1) Antibody (NB100-1194):
Western blot Protocol:
1) Transfer onto PVDF membrane
2) Block in 3% (w/v) skimmed milk in TBS-T (0.05% Tween 20) for 1 hr at room temperature with agitation (can be blocked overnight at 4C without agitation).
3) Incubate NB100-1194 (2ug/ml) for 1 hr at room temperature with agitation.
4) Wash with TBS-T three times
5) Incubate with secondary antibody (1:20,000 or higher). We use Jackson or Rockland anti-goat secondary (affinity purified and with minimal cross-reactivity with Human and rodent serum proteins) for 1 hr at room temperature with agitation.
6) Wash with TBST three times.
7) Apply the detection reagent of choice in accordance with the manufacturers instructions (We use ECL-plus (Amersham) rather than ECL, which is more sensitive for detection.)
Notes:
*All antibodies are incubated in blocking buffer.
*QC testing was done under reducing conditions (with beta mercaptoethanol at 100C).
Western blot Protocol:
1) Transfer onto PVDF membrane
2) Block in 3% (w/v) skimmed milk in TBS-T (0.05% Tween 20) for 1 hr at room temperature with agitation (can be blocked overnight at 4C without agitation).
3) Incubate NB100-1194 (2ug/ml) for 1 hr at room temperature with agitation.
4) Wash with TBS-T three times
5) Incubate with secondary antibody (1:20,000 or higher). We use Jackson or Rockland anti-goat secondary (affinity purified and with minimal cross-reactivity with Human and rodent serum proteins) for 1 hr at room temperature with agitation.
6) Wash with TBST three times.
7) Apply the detection reagent of choice in accordance with the manufacturers instructions (We use ECL-plus (Amersham) rather than ECL, which is more sensitive for detection.)
Notes:
*All antibodies are incubated in blocking buffer.
*QC testing was done under reducing conditions (with beta mercaptoethanol at 100C).
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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