Phospho-STAT6 (Y641) Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody..
  • Species Reactivity
  • Specificity
    Detects human STAT6 when phosphorylated at Y641.
  • Source
    Recombinant Monoclonal Rabbit IgG Clone # 1248D
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    Phosphopeptide containing human STAT6 Y641 site.
    Accession # P42226
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Phospho-STAT6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) Th2-stimulated or (B) unstimulated were stained with Rabbit Anti-Human Phospho-STAT6 (Y641) Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC37173R) and Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # IC1051R). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: STAT6
Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.
  • Long Name:
    Signal Transducer and Activator of Transcription 6
  • Entrez Gene IDs:
    6778 (Human); 20852 (Mouse); 362896 (Rat)
  • Alternate Names:
    D12S1644; EC; EC; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6B; STAT6C; transcription factor IL-4 STAT
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rabbit IgG Alexa Fluor® 647-conjugated Antibody

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