Phospho-STAT6 (Y641) Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC37173R-025
Detection  of Phospho-STAT6 in Human PBMCs by Flow Cytometry.
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Phospho-STAT6 (Y641) Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human STAT6 when phosphorylated at Y641.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1248D
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing human STAT6 Y641 site.
Accession # P42226
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of Phospho-STAT6 in Human PBMCs by Flow Cytometry. View Larger

Detection of Phospho-STAT6 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either (A) Th2-stimulated or (B) unstimulated were stained with Rabbit Anti-Human Phospho-STAT6 (Y641) Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC37173R) and Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P). Quadrant markers were set based on control antibody staining (Catalog # IC1051R). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN4GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.

Long Name
Signal Transducer and Activator of Transcription 6
Entrez Gene IDs
6778 (Human); 20852 (Mouse); 362896 (Rat)
Alternate Names
D12S1644; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT

Product Datasheets

FAQs

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