Phosphoserine Antibody - BSA Free

Novus Biologicals | Catalog # NB100-1953

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Non-species specific

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Phosphoserine Primary Antibodies »

Product Specifications

Immunogen

Phosphoserine conjugated to KLH, and phosvitin mixture

Specificity

Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Phosphoserine Antibody - BSA Free

Western Blot: Phosphoserine Antibody [NB100-1953]

Western Blot: Phosphoserine Antibody [NB100-1953]

Western Blot: Phosphoserine Antibody [NB100-1953] - Western blot analysis of Mouse Spleen lysates showing detection of Phosphoserineprotein using Rabbit Anti-PhosphoserinePolyclonal Antibody (NB100-1953). Primary Antibody: Rabbit Anti-PhosphoserinePolyclonal Antibody (NB100-1953) at 1:1000. Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1uM okadaic acid).
Immunocytochemistry/ Immunofluorescence: Phosphoserine Antibody [NB100-1953]

Immunocytochemistry/ Immunofluorescence: Phosphoserine Antibody [NB100-1953]

Immunocytochemistry/Immunofluorescence: Phosphoserine Antibody [NB100-1953] - Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Phosphoserine Polyclonal Antibody at 1:50 for 12 hours at 4 degrees C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x.
Western Blot: Phosphoserine Antibody [NB100-1953]

Western Blot: Phosphoserine Antibody [NB100-1953]

Western Blot: Phosphoserine Antibody [NB100-1953] - ~30 ug SY5Y or N2A whole cell lysate separated on 8% PAGE, blotted and probed with phosphoserine antibody (1:125 in 3% BSA in TBST). Western blot image submitted by a verified customer review.

Applications for Phosphoserine Antibody - BSA Free

Application
Recommended Usage

ELISA

1:250

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Immunoprecipitation

1:100

Western Blot

1:500
Application Notes
Phosphoserine antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB100-1953 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS, 50% Glycerol

Format

BSA Free

Preservative

0.01 mg Sodium Azide

Concentration

0.25 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Phosphoserine

Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).

Alternate Names

Phosphoserine; Phosphothreonine

Additional Phosphoserine Products

Product Documents for Phosphoserine Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Phosphoserine Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Phosphoserine Antibody - BSA Free

Customer Reviews for Phosphoserine Antibody - BSA Free (1)

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Customer Images

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  • Phosphoserine Antibody
    Name: Marcel Alavi
    Application: Western Blot
    Sample Tested: Mouse Neuro2A whole cell lysate, SHSY5Y whole cell lysate and SH-SY5Y whole cell lysate
    Species: Mouse and Human
    Verified Customer | Posted 02/22/2021
    ~30 µg SY5Y or N2A whole cell lysate separated on 8% PAGE, blotted and probed with phosphoserine AB (1:125 in 3% BSA in TBST).
    Phosphoserine Antibody - BSA Free NB100-1953

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Protocols

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