PLTP Antibody - BSA Free

Novus Biologicals | Catalog # NB400-106

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PLTP Primary Antibodies »

Product Specifications

Immunogen

A partial peptide of human PLTP. [UniProt# P55058]

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit PLTP Antibody - BSA Free (NB400-106) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PLTP Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for PLTP Antibody - BSA Free

Western Blot: PLTP Antibody [NB400-106]

Western Blot: PLTP Antibody [NB400-106]

Western Blot: PLTP Antibody [NB400-106] - Activation of PLTP in STAT3 pathway. RA-FLS were stimulated for 24 hours with rhPLTP at the indicated concentrations. Western blot analyses analyzed cell lysates for phosphorylation of STAT3 (Tyr705). Band intensities were normalized to corresponding STAT3 band intensities. Representative Western blots are shown (n = 3).Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.
Immunocytochemistry/ Immunofluorescence: PLTP Antibody [NB400-106]

Immunocytochemistry/ Immunofluorescence: PLTP Antibody [NB400-106]

Immunocytochemistry/Immunofluorescence: PLTP Antibody [NB400-106] - PLTP antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Immunohistochemistry: PLTP Antibody [NB400-106]

Immunohistochemistry: PLTP Antibody [NB400-106]

PLTP-Antibody-Immunohistochemistry-NB400-106-img0008.jpg
Western Blot: PLTP Antibody [NB400-106]

Western Blot: PLTP Antibody [NB400-106]

Western Blot: PLTP Antibody [NB400-106] - WB analysis of PLTP in human brain lysate.
Immunohistochemistry: PLTP Antibody [NB400-106]

Immunohistochemistry: PLTP Antibody [NB400-106]

PLTP-Antibody-Immunohistochemistry-NB400-106-img0003.jpg
Immunohistochemistry: PLTP Antibody [NB400-106]

Immunohistochemistry: PLTP Antibody [NB400-106]

Immunohistochemistry: PLTP Antibody [NB400-106] - Recombinant PLTP antibody induced cytokine production and FLS proliferation, independent of its lipid transfer ability. (A) RA-FLS were stimulated for 48 hours with PLTP or heat inactivated-PLTP and proliferation was evaluated during the final day of stimulation using thymidine incorporation. (B) Blockade of PLTP decreased the effect of rhPLTP on FLS proliferation. (C) PLTP impact on RA-FLS cytokine production. (D) OA-FLS were treated with either native PLTP or heated-PLTP and analyzed for cytokine production and proliferation. Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.
Immunohistochemistry-Paraffin: PLTP Antibody [NB400-106]

Immunohistochemistry-Paraffin: PLTP Antibody [NB400-106]

Immunohistochemistry-Paraffin: PLTP Antibody [NB400-106] - PLTP was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human PLTP polyclonal Antibody (Catalog # NB400-106) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in decidual cells.
PLTP Antibody

Immunohistochemistry: PLTP Antibody [NB400-106] -

Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP expression in RA synovial tissues.For immuno-histological analysis, (A) synovial tissue sections from RA patients (n = 5) were stained for PLTP or macrophages (CD68+ cells). Representative images obtained for immunohistological staining are shown. Blue arrows show macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with morphological features & CD68+ staining (black arrow; non-exhaustive). (B, C) Double staining was performed to visualize localization (n = 3) of PLTP with macrophages (CD68+) (B) or RA-FLS ( alpha -SMA+) (C). Fluorescence was analyzed at 20x magnification. Overlay is shown to visualize co-localization of PLTP in macrophages (CD68+) (B) or RA-FLS ( alpha -SMA+) (C) or PLTP expression in infiltrate. Original magnification: 20x. Separate images can be found in S1 Fig. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0193815), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PLTP Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

1:500
Application Notes
This PLTP antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western Blot, where a band is seen at ~55kDa representing PLTP. It does not exhibit neutralizing activity. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NB400-106 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PLTP

PLTP (phospholipid transfer protein) belongs to lipid transfer/lipopolysaccharide-binding proteins (BPI/LBP) family and it is a monomeric sectered protein expressed ubiquitously with highest expression in liver, one of the major sites of lipoprotein production and degradation. PLTP converts HDL into larger and smaller particles, and play a key role in extracellular phospholipid transport as well as modulation of HDL particles. Plasma PLTP is also considered a nonspecific lipid transfer protein because, along with phospholipids, it can also transfer alpha-tocopherol, diacylglycerol, cerebroside, and lipopolysaccharides. PLTP deficient mice depicts lowered susceptibility for atherosclerosis, whereas, PLTP transgenic mice have more atherosclerosis. In humans, an increased PLTP activity been linked to several conditions including type I/II diabetes mellitus, metabolic syndrome, obesitas, increased risk of coronary artery disease and cardiovascular disease.

Long Name

Phospholipid Transfer Protein

Alternate Names

HDLCQ9, Lipid transfer protein II, phospholipid transfer protein

Entrez Gene IDs

5360 (Human); 18830 (Mouse)

Gene Symbol

PLTP

UniProt

P55058 (Human)

Additional PLTP Products

Product Documents for PLTP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PLTP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PLTP Antibody - BSA Free

Customer Reviews for PLTP Antibody - BSA Free (1)

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  • PLTP Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Liver
    Species: Mouse
    Verified Customer | Posted 03/06/2018
    Alcohol feeding significantly up-regulated PLTP expression in mouse liver.
    PLTP Antibody - BSA Free NB400-106

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