Porcine CD34 Biotinylated Antibody

Name: Porcine CD34 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF3890
Price: 569 USD
Availability: InStock

R&D Systems | Catalog # BAF3890

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
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Research Areas
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Key Product Details

Species Reactivity

Porcine

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all CD34 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant porcine CD34
Ala20-Thr294 (Thr32Ala, Ala52Thr, Pro71Ser, Val126Ala, His278Asn)
Accession # NP_999251

Specificity

Detects porcine CD34 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant rat CD34 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Porcine CD34 Biotinylated Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Porcine CD34

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: CD34

CD34 is a 110 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucin. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Porcine CD34 is 389 amino acids (aa) in length and is predicted to have a 272 aa extracellular domain (ECD) and a 73 aa cytoplasmic tail. An alternate 307 aa splice form with a 4 aa substitution for the C-terminal 61 amino acids also exists. Over aa 20‑294, porcine CD34 shares 51%, 57% and 62% aa sequence identity with mouse, human, and canine CD34, respectively.