Key Product Details

Species Reactivity

Validated:

Porcine

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD34 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant porcine CD34
Ala20-Thr294 (Thr32Ala, Ala52Thr, Pro71Ser, Val126Ala, His278Asn)
Accession # NP_999251

Specificity

Detects porcine CD34 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross‑reactivity with recombinant rat CD34 is observed, and less than 5% cross-reactivity with recombinant mouse CD34 and recombinant human CD34 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Porcine CD34 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Porcine CD34

Reviewed Applications

Read 1 review rated 5 using AF3890 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD34

CD34 is a 110 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucin. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Porcine CD34 is 389 amino acids (aa) in length and is predicted to have a 272 aa extracellular domain (ECD) and a 73 aa cytoplasmic tail. An alternate 307 aa splice form with a 4 aa substitution for the C-terminal 61 amino acids also exists. Over aa 20-294, porcine CD34 shares 51%, 57% and 62% aa sequence identity with mouse, human, and canine CD34, respectively.

Alternate Names

CD34, HPCA1

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Gene Symbol

CD34

UniProt

NP_999251

Additional CD34 Products

Product Documents for Porcine CD34 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Porcine CD34 Antibody

For research use only

Citations for Porcine CD34 Antibody

Customer Reviews for Porcine CD34 Antibody (1)

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  • Porcine CD34 Antibody
    Name: Hanane Belhoul-Fakir
    Application: Immunohistochemistry-Frozen
    Sample Tested: porcine arteries and Arteries
    Species: Porcine
    Verified Customer | Posted 12/03/2020
    Expression of CD34 (purple stain) in the healthy pig carotid (A, B), abdominal aorta (C) and coronary artery (D). Control with nuclei staining only (E). Intima: In, Media: Me, Adventitia: Ad
    Pig healthy arterial sections of 7µm were fixed in ice cold acetone for 7min. Once dry, blocking buffer 4% FCS/TBS was added to all tissues for 30min to minimize non-specific binding, followed by 10 min incubation with Bloxall, endogenous peroxidase blocking solution at room temperature. Tissues were washed in Tris buffered saline (TBS) for 5 min and incubated for 20min with normal horse serum (Vector laboratories # S-2012-50). Polyclonal goat anti-CD34 (R&D systems # AF3890) was diluted at 1:80 in 0.5% FCS/TBS and added to the arterial tissues for 1 hour incubation at room temperature. Following 5 min wash in TBS, tissues were incubated for 30min with ImmPRESS®HRP horse anti-goat reagent (Vector laboratories # MP-7405). To detect CD34 antigen, tissues were washed in TBS and incubated with VIP HRP substrate (Vector®VIP Substrate kit, Peroxidase (HRP) # SK-4600) for 1 min 30sec then placed immediately in MilliQ water to stop the reaction. Nuclei were counter-stained with methyl green (Sigma-Aldrich # M8884-25G). Control tissues were stained similarly in the absence of primary antibodies. Tissue sections were immediately dehydrated and mounted with ENTELLAN mounting medium (Proscitech, Cat#IA013). Images were captured on Nikon eclipse ME600 microscope and DS-Fi2 camera.
    Porcine CD34 Antibody AF3890

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