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Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit

Catalog #: PLB00
41 Citations Datasheet / COA / SDS
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Porcine IL-1 beta/IL-1F2 ELISA Standard Curve
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Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary

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Porcine IL-1 beta/IL-1F2 ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

Long Name:
Interleukin 1 beta
Entrez Gene IDs:
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Assay Diluent
  3.   Add 50 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 100 µL Conjugate
  6.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 5 times.
    8. 100 µL Substrate Solution
  8.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 100 µL Stop Solution
  9.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. PRRSV infection activates NLRP3 inflammasome through inducing cytosolic mitochondrial DNA stress
    Authors: H Li, X Yang, Y Song, Q Zhu, Z Liao, Y Liang, J Guo, B Wan, D Bao
    Veterinary Microbiology, 2023-02-03;279(0):109673.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  2. Supplementation With Spirulina Reduces Infarct Size and Ameliorates Cardiac Function in a Pig Model of STEMI
    Authors: G Vilahur, P Sutelman, S Ben-Aicha, G Mendieta, M Radiké, L Schoch, L Casaní, M Borrell-Pa, T Padro, L Badimon
    Frontiers in Pharmacology, 2022-05-03;13(0):891801.
    Species: Porcine
    Sample Types: Plasma
  3. Hemosiderin Accumulation in Liver Decreases Iron Availability in Tachycardia-Induced Porcine Congestive Heart Failure Model
    Authors: M Kasztura, L Kiczak, U Pas?awska, J Bania, A Janiszewsk, A Tomaszek, M Zacharski, A Noszczyk-N, R Pas?awski, A Tabi?, P Kuropka, P Dzi?giel, P Ponikowski
    International Journal of Molecular Sciences, 2022-01-18;23(3):.
    Species: Porcine
    Sample Types: Tissue Homogenates
  4. Identification of Virulence Associated Region during Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus during Attenuation In Vitro: Complex Question with Different Strain Backgrounds
    Authors: Y Jiang, W Tong, L Yu, L Li, F Gao, G Li, C Liu, P Chen, Q Shen, Y Zhang, Y Zhou, G Tong
    Viruses, 2021-12-27;14(1):.
    Species: Porcine
    Sample Types: Serum
  5. A novel pre-clinical strategy to deliver antimicrobial doses of inhaled nitric oxide
    Authors: VS Michaelsen, RVP Ribeiro, E Brambate, A Ali, A Wang, L Pires, M Kawashima, Y Zhang, A Gazzalle, S Keshavjee, L Del Sorbo, M Cypel
    PLoS ONE, 2021-10-13;16(10):e0258368.
    Species: Porcine
    Sample Types: Tissue Homogenates
  6. Epigenetic Modulation of TLR4 Expression by Sulforaphane Increases Anti-Inflammatory Capacity in Porcine Monocyte-Derived Dendritic Cells
    Authors: X Qu, C Neuhoff, MU Cinar, M Pröll, E Tholen, D Tesfaye, M Hölker, K Schellande, MJ Uddin
    Biology, 2021-05-31;10(6):.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  7. Thymol modulates the endocannabinoid system and gut chemosensing of weaning pigs
    Authors: A Toschi, B Tugnoli, B Rossi, A Piva, E Grilli
    BMC Vet. Res., 2020-08-12;16(1):289.
    Species: Porcine
    Sample Types: Tissue Homogenates, Tissue Lysates
  8. Basic information for the development of a toxicity assay in inactivated bacterial vaccines
    Authors: EM Colella, GL Alborali, S Dotti, M Amadori
    Res. Vet. Sci., 2020-07-31;132(0):386-392.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  9. Effects of sustained inflation pressure during�neonatal cardiopulmonary resuscitation of asphyxiated piglets
    Authors: GH Shim, SY Kim, PY Cheung, TF Lee, M O'Reilly, GM Schmölzer
    PLoS ONE, 2020-06-23;15(6):e0228693.
    Species: Porcine
    Sample Types: Tissue Homogenates
  10. Substitution of warthog NF-kappaB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus
    Authors: S McCleary, R Strong, RR McCarthy, JC Edwards, EL Howes, LM Stevens, PJ Sánchez-Co, A Núñez, S Watson, AJ Mileham, SG Lillico, C Tait-Burka, C Proudfoot, M Ballantyne, CBA Whitelaw, F Steinbach, HR Crooke
    Sci Rep, 2020-06-02;10(1):8951.
    Species: Porcine
    Sample Types: Serum
  11. Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model
    Authors: SE Pischke, S Hestenes, HT Johansen, H Fure, JF Bugge, A Espinoza, H Skulstad, T Edvardsen, E Fosse, TE Mollnes, PS Halvorsen, EW Nielsen
    PLoS ONE, 2019-06-27;14(6):e0218624.
    Species: Porcine
    Sample Types: Plasma
  12. PO2 oscillations induce lung injury and inflammation
    Authors: S Boehme, EK Hartmann, T Tripp, SC Thal, M David, D Abraham, JE Baumgardne, K Markstalle, KU Klein
    Crit Care, 2019-03-27;23(1):102.
    Species: Porcine
    Sample Types: Plasma
  13. Medium-Chain Triglycerides Attenuate Liver Injury in Lipopolysaccharide-Challenged Pigs by Inhibiting Necroptotic and Inflammatory Signaling Pathways
    Authors: L Zhang, X Wang, S Chen, S Wang, Z Tu, G Zhang, H Zhu, X Li, J Xiong, Y Liu
    Int J Mol Sci, 2018-11-21;19(11):.
    Species: Porcine
    Sample Types: Serum
  14. Pulmonary Effects of Remote Ischemic Preconditioning in a Porcine Model of Ventilation-induced Lung Injury
    Authors: A Bergmann, T Schilling, G Hedenstier, K Ahlgren, A Larsson, M Kretzschma, A Kozian, T Hachenberg
    Respir Physiol Neurobiol, 2018-08-31;0(0):.
    Species: Porcine
    Sample Types: Serum
  15. Inflammatory markers before and after farrowing in healthy sows and in sows affected with postpartum dysgalactia syndrome
    Authors: M Kaiser, M Jacobson, PH Andersen, P Bækbo, JJ Cerón, J Dahl, D Escribano, S Jacobsen
    BMC Vet. Res., 2018-03-12;14(1):83.
    Species: Porcine
    Sample Types: Serum
  16. Influence of zinc supplementation on immune parameters in weaned pigs
    Authors: V Kloubert, K Blaabjerg, TS Dalgaard, HD Poulsen, L Rink, I Wessels
    J Trace Elem Med Biol, 2018-01-31;0(0):.
    Species: Porcine
    Sample Types: Whole Blood
    Applications: ELISA Capture
  17. An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome
    Authors: Angela J Westover
    J Diabetes Res, 2016-10-13;2016(0):3486727.
    Species: Porcine
    Sample Types: Plasma
  18. Sulforaphane epigenetically regulates innate immune responses of porcine monocyte-derived dendritic cells induced with lipopolysaccharide.
    Authors: Qu X, Proll M, Neuhoff C, Zhang R, Cinar M, Hossain M, Tesfaye D, Grosse-Brinkhaus C, Salilew-Wondim D, Tholen E, Looft C, Holker M, Schellander K, Uddin M
    PLoS ONE, 2015-03-20;10(3):e0121574.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  19. Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig.
    Authors: Yeung JC, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun YM, Hu J, Waddell TK, Hwang DM, Liu M, Keshavjee S
    Mol. Ther., 2012-03-27;20(6):1204-11.
    Species: Porcine
    Sample Types: Plasma
  20. Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans.
    Authors: Barratt-Due A, Thorgersen EB, Lindstad JK, Pharo A, Lissina O, Lambris JD, Nunn MA, Mollnes TE
    J. Immunol., 2011-09-30;187(9):4913-9.
    Species: Porcine
    Sample Types: Plasma
  21. Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis.
    Authors: Hellerud BC, Nielsen EW, Thorgersen EB, Lindstad JK, Pharo A, Tonnessen TI, Castellheim A, Mollnes TE, Brandtzaeg P
    Crit. Care Med., 2010-06-01;38(6):1467-74.
    Species: Porcine
    Sample Types: Plasma
  22. Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine.
    Authors: McILwain RB, Timpa JG, Kurundkar AR
    Lab. Invest., 2009-11-09;90(1):128-39.
    Species: Porcine
    Sample Types: Plasma
  23. Cardiac Function and the Proinflammatory Cytokine Response After Recovery From Cardiac Arrest in Swine.
    Authors: Niemann JT, Rosborough JP, Youngquist S, Shah AP, Lewis RJ, Phan QT, Filler SG
    J. Interferon Cytokine Res., 2009-11-01;0(0):.
    Species: Porcine
    Sample Types: Plasma
  24. CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
    Authors: Thorgersen EB, Hellerud BC, Nielsen EW, Barratt-Due A, Fure H, Lindstad JK, Pharo A, Fosse E, Tonnessen TI, Johansen HT, Castellheim A, Mollnes TE
    FASEB J., 2009-10-19;24(3):712-22.
    Species: Porcine
    Sample Types: Plasma
  25. Metabolic activity of the enteric microbiota influences the fatty acid composition of murine and porcine liver and adipose tissues.
    Authors: Wall R, Ross RP, Shanahan F, O'Mahony L, O'Mahony C, Coakley M, Hart O, Lawlor P, Quigley EM, Kiely B, Fitzgerald GF, Stanton C
    Am. J. Clin. Nutr., 2009-04-08;89(5):1393-401.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  26. Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.
    Authors: Thorgersen EB, Pharo A, Haverson K, Axelsen AK, Gaustad P, Kotwal GJ, Sfyroera G, Mollnes TE
    Infect. Immun., 2008-12-01;77(2):725-32.
    Species: Porcine
    Sample Types: Whole Blood
  27. Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery.
    Authors: Kruse R, Essen-Gustavsson B, Fossum C, Jensen-Waern M
    Acta Vet. Scand., 2008-08-12;50(0):32.
    Species: Porcine
    Sample Types: Serum
  28. Effects of glucocorticoids in ventilated piglets with severe pneumonia.
    Authors: Sibila O, Luna CM, Agusti C, Baquero S, Gando S, Patron JR, Morato JG, Absi R, Bassi N, Torres A
    Eur. Respir. J., 2008-05-28;32(4):1037-46.
    Species: Porcine
    Sample Types: Serum
  29. Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model.
    Authors: Saethre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, Mollnes TE
    J. Immunol., 2008-05-01;180(9):6346-53.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  30. Experimental Pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response.
    Authors: Sibila O, Agusti C, Torres A, Baquero S, Gando S, Patron JR, Morato JG, Goffredo DH, Bassi N, Luna CM
    Eur. Respir. J., 2007-09-05;30(6):1167-72.
    Species: Porcine
    Sample Types: Serum
  31. The fibrin-derived peptide Bbeta15-42 is cardioprotective in a pig model of myocardial ischemia-reperfusion injury.
    Authors: Roesner JP, Petzelbauer P, Koch A, Mersmann J, Zacharowski PA, Boehm O, Reingruber S, Pasteiner W, Mascher D, Wolzt M, Barthuber C, Noldge-Schomburg GE, Scheeren TW, Zacharowski K
    Crit. Care Med., 2007-07-01;35(7):1730-5.
    Species: Porcine
    Sample Types: Plasma
  32. Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate.
    Authors: Zhang F, Hopwood P, Abrams CC, Downing A, Murray F, Talbot R, Archibald A, Lowden S, Dixon LK
    J. Virol., 2006-11-01;80(21):10514-21.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  33. Complete overlap of interleukin-31 receptor A and oncostatin M receptor beta in the adult dorsal root ganglia with distinct developmental expression patterns.
    Authors: Bando T, Morikawa Y, Komori T, Senba E
    Neuroscience, 2006-08-22;142(4):1263-71.
    Species: Porcine
    Sample Types: Plasma
  34. Effects of therapeutic bronchoalveolar lavage and partial liquid ventilation on meconium-aspirated newborn piglets.
    Authors: Jeng MJ, Soong WJ, Lee YS, Chang HL, Shen CM, Wang CH, Yang SS, Hwang B
    Crit. Care Med., 2006-04-01;34(4):1099-105.
    Species: Porcine
    Sample Types: Serum
  35. Protection against acute porcine lung ischemia/reperfusion injury by systemic preconditioning via hind limb ischemia.
    Authors: Waldow T, Alexiou K, Witt W, Albrecht S, Wagner F, Knaut M, Matschke K
    Transpl. Int., 2005-02-01;18(2):198-205.
    Species: Porcine
    Sample Types: Plasma
  36. Deciphering the involvement of innate immune factors in the development of the host response to PRRSV vaccination.
    Authors: Royaee AR, Husmann RJ, Dawson HD, Calzada-Nova G, Schnitzlein WM, Zuckermann FA, Lunney JK
    Vet. Immunol. Immunopathol., 2004-12-08;102(3):199-216.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  37. Decreased protein accretion in pigs with viral and bacterial pneumonia is associated with increased myostatin expression in muscle.
    Authors: Escobar J, Van Alstine WG, Baker DH
    J. Nutr., 2004-11-01;134(11):3047-53.
    Species: Porcine
    Sample Types: Serum
  38. Vitamin E supplementation does not mitigate the acute morbidity effects of porcine reproductive and respiratory syndrome virus in nursery pigs.
    Authors: Toepfer-Berg TL, Escobar J, Van Alstine WG, Baker DH, Salak-Johnson J, Johnson RW
    J. Anim. Sci., 2004-07-01;82(7):1942-51.
    Species: Porcine
    Sample Types: Serum
  39. Attenuation of reperfusion-induced systemic inflammation by preconditioning with nitric oxide in an in situ porcine model of normothermic lung ischemia.
    Authors: Waldow T, Alexiou K, Witt W, Wagner FM, Gulielmos V, Matschke K, Knaut M
    Chest, 2004-06-01;125(6):2253-9.
    Species: Porcine
    Sample Types: Plasma
  40. The effect of warm humidified CO2 on the dissipation of residual gas following laparoscopy in piglets.
    Authors: Glew PA, Campher MJ, Pearson K, Schofield JC, Davey AK
    J Am Assoc Gynecol Laparosc, 2004-05-01;11(2):204-10.
    Species: Porcine
    Sample Types: Serum
  41. Tumour necrosis factor alpha stimulates nitric oxide production more potently than interleukin-1beta in porcine articular chondrocytes.
    Authors: Goodstone NJ, Hardingham TE
    Rheumatology (Oxford), 2002-08-01;41(8):883-91.
    Species: Porcine
    Sample Types: Cell Culture Supernates

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