Presenilin-1 Antibody (APS 11)

Novus Biologicals | Catalog # NB300-749

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # APS 11
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Product Specifications

Immunogen

Synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Integral membrane protein. Golgi and endoplasmic reticulum. Bound to NOTCH1 also at the cell surface.

Specificity

No cross-reactivity is seen with presenilin 2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Presenilin-1 Antibody (APS 11)

Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749]

Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749]

Western Blot: Presenilin-1 Antibody (APS 11) [NB300-749] - Analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3).
Immunocytochemistry/ Immunofluorescence: Presenilin-1 Antibody (APS 11) [NB300-749]

Immunocytochemistry/ Immunofluorescence: Presenilin-1 Antibody (APS 11) [NB300-749]

Immunocytochemistry/Immunofluorescence: Presenilin-1 Antibody (APS 11) [NB300-749] - Mouse fibroblasts.
Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749]

Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749]

Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human liver tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749]

Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749]

Immunohistochemistry-Paraffin: Presenilin-1 Antibody (APS 11) [NB300-749] - Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Presenilin 1 or without primary antibody (negative control) overnight at 4C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Tissues were counterstained with hematoxylin and prepped for mounting.

Applications for Presenilin-1 Antibody (APS 11)

Application
Recommended Usage

ELISA

4.5 ug/ml

Immunocytochemistry/ Immunofluorescence

0.75 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Paraffin

0.75 ug/ml

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:1000
Application Notes
IHC-P: Staining of both the human brain plaque core and dystrophic neurites. WB: Detects an approx. 28 kDa protein representing PS1 N-terminus cleavage product.

Reviewed Applications

Read 1 review rated 4 using NB300-749 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Presenilin-1

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2). Presenilins form the catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Evidence suggests cleavage of APP by gamma-secretase and the production of Abeta (42 aa) is likely to play a important or casual role in the development of Alzheimer's disease.

Alternate Names

Presenilin1, PSEN1, S182

Entrez Gene IDs

5663 (Human); 19164 (Mouse); 29192 (Rat)

Gene Symbol

PSEN1

UniProt

P49768

Additional Presenilin-1 Products

Product Documents for Presenilin-1 Antibody (APS 11)

Certificate of Analysis

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Product Specific Notices for Presenilin-1 Antibody (APS 11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Presenilin-1 Antibody (APS 11)

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    Application: Western Blot
    Verified Customer | Posted 12/07/2012
    MT2 protects neurons from Ab amyloid-mediated death in NGF-deficient neurons
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Protocols

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FAQs for Presenilin-1 Antibody (APS 11)

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  • Q: What is the exact immuogen?

    A: The immunogen range for this antibody falls between amino acids 340-370.

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