ProDots Recombinant Human IL-15 GMP Protein
ProDots Recombinant Human IL-15 GMP Protein Summary
The specific activity of ProDots Recombinant Human IL-15 GMP is >2.0 x 108 units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554).
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from an phosphate buffered saline-based formulation using proprietary excipients.|
|Reconstitution||Reconstitute with cell culture media immediately prior to use.|
|Shipping||The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Store material as supplied, unopened, and unreconstituted at 2-8 °C until use. Stability is a minimum of 8 weeks when stored at 2-8 °C as supplied. Refer to lot specific COA for the Use by Date.
The foil pouch is used for shipping and storage only and is not terminally sterilized. GMP ProDots within the weldable bag are prepared under GMP-controlled conditions and protein content is tested to USP <71> guidelines.
GMP-grade ProDots®Recombinant Human IL-15 (Catalog # PRD247-GMP) stimulates cell proliferation of MO7e human megakaryocytic leukemic cells. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP ProDots IL-15.
Comparative tests for function within biologically relevant model systems show equivalence of GMP ProDot™ Proteins and GMP Proteins. Activation of PBMCs after 9 days in culture with ExCellerate Human T Cell Media CCM030, GMP Cloudz Human T Cell Activation Kit CLD001-GMP and GMP IL-15 ProDot PRD247-GMP or GMP IL-15 247-GMP shows comparable A. T cell fold expansion and B. phenotypes
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1, 2). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternative splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (3). IL-15 binds with high affinity to IL-15 R alpha (4). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (5). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (6). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (7). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (6). In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 in circulation (8-10). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (4, 9). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (11). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells (12 - 16). An important component of these functions is the ability of
IL-15 to induce dendritic cell differentiation and inflammatory activation (11, 14). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8+ T cells (17). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (18).
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