Mouse EGF R/ErbB1 Antibody

Name: Mouse EGF R/ErbB1 Antibody
Brand: R&D Systems
SKU: MAB1280

Catalog #: MAB1280
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB1280 has been discontinued.

View all EGFR products.

All EGFR Antibodies

Product Details

Citations (1)

FAQs

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse EGF R/ErbB1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse EGF R in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows no cross‑reactivity with recombinant human (rh) EGF R, rhErbB2, rhErbB3, or rhErbB4.

Source

Monoclonal Rat IgG₁ Clone # 176436

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EGF R
Leu25-Ser647
Accession # Q01279

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse EGF R/ErbB1 Fc Chimera (Catalog # 1280-ER)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGFR

The EGF R subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER-1, ErbB1, or ErbB), ErbB2 (Neu, HER-2), ErbB3 (HER-3), and ErbB4 (HER-4). All family members are type I transmembrane glycoproteins. They contain an extracellular ligand binding domain containing two cysteine-rich domains and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain followed by multiple tyrosine autophosphorylation sites (1, 2). The mouse EGF R cDNA encodes a 1210 amino acid (aa) precursor with a 24 aa signal peptide, a 623 aa extracellular domain (ECD), a 23 aa transmembrane segment, and a 540 aa cytoplasmic domain (3). Soluble receptors consisting of the extracellular ligand binding domain are generated by alternate splicing in human and mouse (4 - 6). Within the ECD, mouse EGF R shares 88% and 93% aa sequence identity with human and rat EGF R, respectively. It shares 44% - 48% aa sequence identity with the ECD of mouse ErbB2, ErbB3, and ErbB4. EGF R binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen (1, 2). Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, heterodimerization tyrosine phosphorylation and cell signaling (7 - 11). EGF R can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGF R signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis (12, 13). EGF R is over-expressed in a wide variety of tumors and is the target of several anti-cancer drugs (14).

References

Singh, A.B. and R.C. Harris (2005) Cell. Signal. 17:1183.
Shilo, B.Z. (2005) Development 132:4017.
Avivi, A. et al. (1991) Oncogene 6:673.
Reiter, J.L. and N.J. Maihle (1996) Nucleic Acids Res. 24:4050.
Reiter J.L. et al. (2001) Genomics 71:1.
Xu, Y.H. et al. (1984) Nature 309:806.
Graus-Porta, D. et al. (1997) EMBO J. 16:1647.
Yarden, Y. et al. (1987) Biochemistry 26:1434.
Burgess, A.W. et al. (2003) Mol. Cell 12:541.
Lemmon, M.A. et al. (1997) EMBO J. 16:281.
Cohen, S. et al. (1982) J. Biol. Chem. 257:1523.
Sibilia, M. and E.F. Wagner (1995) Science 269:234.
Miettinen, P.J. et al. (1995) Nature 376:337.
Roskoski Jr., R. (2004) Biochem. Biophys. Res. Commun. 319:1.

Long Name

Epidermal Growth Factor Receptor

Entrez Gene IDs

1956 (Human); 13649 (Mouse); 24329 (Rat); 102138724 (Cynomolgus Monkey)

Alternate Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse EGF R/ErbB1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Thalidomide inhibits epidermal growth factor-induced cell growth in mouse and human monocytic leukemia cells via Ras inactivation.
Authors: Noman AS, Koide N, Khuda II, Dagvadorj J, Tumurkhuu G, Naiki Y, Komatsu T, Yoshida T, Yokochi T
Biochem. Biophys. Res. Commun., 2008-07-26;374(4):683-7.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: Flow Cytometry, Western Blot