Quantitation of Proteome Profiler™ Antibody Arrays with FluorChem™ Imaging Systems

Proteome Profiler Antibody Array Kits utilize chemiluminescence for detection of multiple analytes in one sample. Many of our kits can also be adapted for near-infrared (NIR) fluorescence detection by replacing the HRP-conjugated Streptavidin provided in the kit with IRDye® 800CW Streptavidin. This modification is not applicable to array kits that use an HRP-conjugated detection antibody instead of Streptavidin-HRP, and it is not recommended for arrays with phospho-specific antibodies due to increased signal noise.

Both chemiluminescence and fluorescence based Proteome Profiler Antibody Array Kits can be quickly and accurately analyzed by FluorChem systems with AlphaView software.

Cytokine Production from HUVEC Cultures with or without TNF-alpha Treatment

Figure 1 Supernatants from human umbilical vein enthothelial cells (HUVEC) with or without TNF-alpha treatment were screened for changes in cytokine expression. Proteome Profiler Human XL Cytokine Array membranes (left; Catalog # ARY022B) were imaged for 10 minutes on a FluorChem R system, and AlphaView software was used to quantitate the change in cytokine and soluble receptor expression (right).


Learn more about how Proteome Profiler Antibody Arrays and FluorChem systems can work together to advance your research. Our App Note contains array protocol details, a description of instrument and software capabilities, and multiple data examples.


Watch our video to learn more about FluorChem systems. See how easy it is to generate high quality images from Western blot membranes.


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Articles using Proteome Profiler Antibody Arrays with FluorChem systems


A Novel Rat Model of Liver Regeneration: Possible Role of Cytokine Induced Neutrophil Chemoattractant-1 in Augmented Liver Regeneration. Dhar, D.K. et al. (2015) Ann. Surg. Innov. Res. 9:11.

  • Profiled expression levels of multiple secreted cytokines at early and later stages of tissue regeneration
  • Rat Cytokine Array Kit (Catalog # ARY008)
  • FluorChem M system


Bacterium-Generated Nitric Oxide Hijacks Host Tumor Necrosis Factor Alpha Signaling and Modulates the Host Cell Cycle In Vitro. Mocca, B. and Wang, W. (2012) J. Bacteriol 194:4059.

  • Profiled cytokines and intracellular apoptosis-related proteins in bronchial epithelial cells
  • Human Cytokine Array Kit (Catalog # ARY005B)
  • Human Apoptosis Array Kit (Catalog # ARY009)
  • FluorChem Q system


PIWI-like Protein, HIWI2 is Aberrantly Expressed in Retinoblastoma Cells and Affects Cell Cycle Potentially Through OTX2. Sivagurunathan, S. et al. (2017) Cell Mol. Biol. Lett. 22:17.

  • Profiled stem cell markers following HIWI2 knockdown in retinoblastoma cells
  • Human Pluripotent Stem Cell Array Kit (Catalog # ARY010)
  • FluorChem FC3 system