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Proteome Profiler Rat Cytokine Array Kit, Panel A

ARY008 43 Citations 8 Reviews Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 29 different cytokine antibodies
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ARY008

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The Rat Cytokine Array detects multiple analytes in cell culture supernates.
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Citations (43)
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Reviews (8)
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Proteome Profiler Rat Cytokine Array Kit, Panel A Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected rat cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.

Troubleshooting Guide

Key Benefits

  • Detects 29 rat cytokines and chemokines simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are required.  Refer to protocol.

Principle of the Assay

The Proteome Profiler Rat Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 
Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Rat Cytokine Array, Panel A nitrocellulose membranes spotted with 29 different antibodies to rat cytokines and chemokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Rat Cytokine Array, Panel A
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

 

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
CXCL1/CINC-1 IL-1ra/IL-1F3 L-Selectin
CXCL3/CINC-2 alpha/beta IL-2 CXCL9/MIG
CXCL2/CINC-3 IL-3 CCL3/MIP-1 alpha
CNTF IL-4 CCL20/MIP-3 alpha
Fractalkine/CXC3CL1 IIL-6 CCL5/RANTES
GM-CSF IL-10 CXCL7/Thymus Chemokine
ICAM-1 IL-13 TIMP-1
IFN-gamma IL-17 TNF-alpha
IL-1 alpha/IL-1F1 CXCL10/IP-10 VEGF
IL-1 beta/IL-1F2 LIX  

 

Assays for Analytes represented in the Rat Cytokine Array Kit, Panel A

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CXCL1/CINC-1

RCN100

 DY515
CXCL3/CINC-2 alpha/beta RCN200 DY516
CXCL2/CINC-3 RCN300 DY525
CNTF   DY557
Fractalkine/CX3CL1 MCX310 DY537
GM-CSF   DY518
ICAM-1 RIC100 DY583
IFN-gamma RIF00 DY585
IL-1 alpha RRA00 DY500
IL-1 beta RLB00 DY501
IL-1ra    
IL-2 R2000 DY502
IL-3    
IL-4 R4000 DY504
IL-6 R6000B DY506
IL-10 R1000 DY522
IL-13    
IL-17    
CXCL10/IP-10    
LIX   DY543
L-Selectin   DY1534
CXCL9/MIG    
CCL3/MIP-1 alpha    
CCL20/MIP-3 alpha   DY540
CCL5/RANTES MMR00  
CXCL7/Thymus Chemokine   DY1116
TIMP-1 RTM100 DY580
TNF-alpha RTA00 DY510
VEGF RRV00 DY564

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Rat

Product Datasheets

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Product Datasheet
COA
SDS

Data Example

The Rat Cytokine Array detects multiple analytes in cell culture supernates. View Larger

The Rat Cytokine Array detects multiple analytes in cell culture supernates. NR8383 rat alveolar macrophage cells were either untreated or treated as below. Data shown are from a two minute exposure to X-ray film. A, untreated. B, Treated with 100 ng/mL LPS for 24 hours. C, Pre-treated with 100 nM Dexamethasone for 72 hours followed by treatment with 100 ng/mL LPS for 24 hours.

The Rat Cytokine Array detects multiple analytes in tissue lysates and serum. View Larger

The Rat Cytokine Array detects multiple analytes in tissue lysates and serum. A rat was injected with 0.1 mg/kg LPS. After 24 hours, tissues were excised and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film (Panels A-B). Tissues were excised from untreated rats and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film (Panels C-E). Serum samples from 15 week old male rats were prepared as described in the Sample Collection and Storage section. 100 µL of serum was run on the array. Data shown are from a 5 minute exposure to X-ray film (Panel F).

The Rat Cytokine Array detects multiple analytes in cell culture supernates and lysates. View Larger

The Rat Cytokine Array detects multiple analytes in cell culture supernates and lysates. (A. and B.) Supernates from NR8383 rat alveolar macrophage cell line untreated or treated with 50 ng/mL LPS for 24 hours. 500 μL of supernate was used for each array shown. (C. and D.) Lysates from NR8383 rat alveolar macrophage cell line untreated or treated with 50 ng/mL LPS for 24 hours. 200 μg of lysates was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

The Rat Cytokine Array detects cytokines present in serum and tissue. View Larger

The Rat Cytokine Array detects cytokines present in serum and tissue. Representative membranes and selective quantification are shown for rat serum (A., 150 μL), lysates of rat lung tissue (B., 400 μg), and lysates of rat liver tissue (C., 400 μg). Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Citations for Proteome Profiler Rat Cytokine Array Kit, Panel A

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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  1. RACK1 plays a critical role in mast cell secretion and Ca2+ mobilization by modulating F-actin dynamics
    Authors: EGF Filho, EZM da Silva, HL Ong, WD Swaim, IS Ambudkar, C Oliver, MC Jamur
    Journal of Cell Science, 2021;134(9):.  2021
  2. Effects of prenatal photobiomodulation treatment on neonatal hypoxic ischemia in rat offspring
    Authors: L Yang, Y Dong, C Wu, H Youngblood, Y Li, X Zong, L Li, T Xu, Q Zhang
    Theranostics, 2021;11(3):1269-1294.  2021
  3. Adenine Nucleotide Translocase 1 Expression Modulates the Immune Response in Ischemic Hearts
    Authors: F Yergöz, J Friebel, N Kränkel, U Rauch-Kroe, HP Schultheis, U Landmesser, A Dörner
    Cells, 2021;10(8):.  2021
  4. Transient Global Ischemia-Induced Brain Inflammatory Cascades Attenuated by Targeted Temperature Management
    Authors: DK Hong, YS Park, JS Woo, JH Kim, JH Beom, SP Chung, JS You, SW Suh
    International Journal of Molecular Sciences, 2021;22(10):.  2021
  5. Peripheral Macrophage-derived Exosomes promote repair after Spinal Cord Injury by inducing Local Anti-inflammatory type Microglial Polarization via Increasing Autophagy
    Authors: B Zhang, F Lin, J Dong, J Liu, Z Ding, J Xu
    International journal of biological sciences, 2021;17(5):1339-1352.  2021
  6. Tspan8-Tumor Extracellular Vesicle-Induced Endothelial Cell and Fibroblast Remodeling Relies on the Target Cell-Selective Response
    Authors: W Mu, J Provaznik, T Hackert, M Zöller
    Cells, 2020;9(2):.  2020
  7. Recombinant Alkaline Phosphatase Prevents Acute on Chronic Liver Failure
    Authors: C Engelmann, D Adebayo, M Oria, F De Chiara, S Novelli, A Habtesion, N Davies, F Andreola, R Jalan
    Sci Rep, 2020;10(1):389.  2020
  8. Beneficial effects of IL-4 and IL-6 on rat neonatal target cardiac cells
    Authors: C Zogbi, NC Oliveira, D Levy, SP Bydlowski, V Bassaneze, EA Neri, JE Krieger
    Sci Rep, 2020;10(1):12350.  2020
  9. Impaired integrin &alpha5 /&beta1 -mediated hepatocyte growth factor release by stellate cells of the aged liver
    Authors: F Rohn, C Kordes, T Buschmann, D Reichert, M Wammers, G Poschmann, K Stühler, AS Benk, F Geiger, JP Spatz, D Häussinger
    Aging Cell, 2020;0(0):e13131.  2020
  10. Theta-burst transcranial magnetic stimulation promotes stroke recovery by vascular protection and neovascularization
    Authors: X Zong, Y Li, C Liu, W Qi, D Han, L Tucker, Y Dong, S Hu, X Yan, Q Zhang
    Theranostics, 2020;10(26):12090-12110.  2020
  11. Differences in pathological changes between two rat models of severe traumatic brain injury
    Authors: YM Song, Y Qian, WQ Su, XH Liu, JH Huang, ZT Gong, HL Luo, C Gao, RC Jiang
    Neural Regen Res, 2019;14(10):1796-1804.  2019
  12. Euphorbia bicolor (Euphorbiaceae) Latex Extract Reduces Inflammatory Cytokines and Oxidative Stress in a Rat Model of Orofacial Pain
    Authors: P Basu, RS Hornung, DL Averitt, C Maier
    Oxid Med Cell Longev, 2019;2019(0):8594375.  2019
  13. The effects of human immunoglobulin G on enhancing tissue protection and neurobehavioral recovery after traumatic cervical spinal cord injury are mediated through the neurovascular unit
    Authors: JCT Chio, J Wang, A Badner, J Hong, V Surendran, MG Fehlings
    J Neuroinflammation, 2019;16(1):141.  2019
  14. Dendrobium huoshanense polysaccharide regulates intestinal lamina propria immune response by stimulation of intestinal epithelial cells via toll-like receptor 4
    Authors: SZ Xie, ZZ Shang, QM Li, XQ Zha, LH Pan, JP Luo
    Carbohydr Polym, 2019;222(0):115028.  2019
  15. CGRP Induces Differential Regulation of Cytokines from Satellite Glial Cells in Trigeminal Ganglia and Orofacial Nociception
    Authors: S Afroz, R Arakaki, T Iwasa, M Oshima, M Hosoki, M Inoue, O Baba, Y Okayama, Y Matsuka
    Int J Mol Sci, 2019;20(3):.  2019
  16. Localized Intrathecal Delivery of Mesenchymal Stromal Cells Conditioned Medium Improves Functional Recovery in a Rat Model of Spinal Cord Injury
    Authors: D Cizkova, V Cubinkova, T Smolek, AN Murgoci, J Danko, K Vdoviakova, F Humenik, M Cizek, J Quanico, I Fournier, M Salzet
    Int J Mol Sci, 2018;19(3):.  2018
  17. EBI2 regulates pro-inflammatory signalling and cytokine release in astrocytes
    Authors: A Rutkowska, DR Shimshek, AW Sailer, KK Dev
    Neuropharmacology, 2018;133(0):121-128.  2018
  18. Paclitaxel�treatment and PC1/3 knockdown in macrophages is a promising anti-glioma strategy as revealed by proteomics and cytotoxicity studies
    Authors: M Duhamel, M Rose, F Rodet, AN Murgoci, L Zografidou, A Régnier-Vi, F Vanden Abe, F Kobeissy, S Nataf, L Pays, M Wisztorski, D Cizkova, I Fournier, M Salzet
    Mol. Cell Proteomics, 2018;0(0):.  2018
  19. Sphingosine 1-phosphate receptors regulate TLR4-induced CXCL5 release from astrocytes and microglia
    Authors: SA O'Sullivan, C O'Sullivan, LM Healy, KK Dev, GK Sheridan
    J. Neurochem., 2018;0(0):.  2018
  20. Aged blood factors decrease cellular responses associated with delayed gingival wound repair
    Authors: MP Saldías, C Fernández, A Morgan, C Díaz, D Morales, F Jaña, A Gómez, A Silva, F Briceño, A Oyarzún, F Maldonado, O Cerda, PC Smith, M Cáceres
    PLoS ONE, 2017;12(9):e0184189.  2017
  21. Immune tuning scaffold for the local induction of a pro-regenerative environment
    Authors: B Corradetti, F Taraballi, C Corbo, F Cabrera, L Pandolfi, S Minardi, X Wang, J Van Eps, G Bauza, B Weiner, E Tasciotti
    Sci Rep, 2017;7(1):17030.  2017
  22. Adaptor protein-3: A key player in RBL-2H3 mast cell mediator release
    Authors: EZ da Silva, EG Freitas-Fi, DA de Souza-J, LL daSilva, MC Jamur, C Oliver
    PLoS ONE, 2017;12(3):e0173462.  2017
  23. Neurotoxic reactive astrocytes are induced by activated microglia
    Authors: SA Liddelow, KA Guttenplan, LE Clarke, FC Bennett, CJ Bohlen, L Schirmer, ML Bennett, AE Mnch, WS Chung, TC Peterson, DK Wilton, A Frouin, BA Napier, N Panicker, M Kumar, MS Buckwalter, DH Rowitch, VL Dawson, TM Dawson, B Stevens, BA Barres
    Nature, 2017;541(7638):481-487.  2017
  24. Acute Mobilization and Migration of Bone Marrow-derived Stem Cells following Anterior Cruciate Ligament Rupture
    Authors: T Maerz, M Fleischer, MD Newton, A Davidson, M Salisbury, P Altman, MD Kurdziel, K Anderson, A Bedi, KC Baker
    Osteoarthr. Cartil, 2017;0(0):.  2017
  25. Activation of NLRP3 inflammasome complex potentiates venous thrombosis in response to hypoxia
    Authors: N Gupta, A Sahu, A Prabhakar, T Chatterjee, T Tyagi, B Kumari, N Khan, V Nair, N Bajaj, M Sharma, MZ Ashraf
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.  2017
  26. Implication of Free Fatty Acids in Thrombin Generation and Fibrinolysis in Vascular Inflammation in Zucker Rats and Evolution with Aging
    Authors: J Lagrange, M Didelot, A Mohamadi, LA Walton, S Bloemen, B de Laat, H Louis, SN Thornton, B Derby, MJ Sherratt, B Fève, P Challande, R Akhtar, JK Cruickshan, P Lacolley, V Regnault
    Front Physiol, 2017;8(0):949.  2017
  27. Cytokine evaluation in untreated and radioimmunotherapy-treated tumors in an immunocompetent rat model
    Authors: E Elgström, TG Ohlsson, SE Eriksson
    Tumour Biol., 2017;39(4):1010428317697.  2017
  28. Ocular Delivery of PACAP1-27 Protects the Retina From Ischemic Damage in Rodents
    Invest. Ophthalmol. Vis. Sci, 2016;57(15):6683-6691.  2016
  29. Myelin phagocytosis by astrocytes after myelin damage promotes lesion pathology
    Authors: Gerald Ponath
    Brain, 2016;0(0):.  2016
  30. Robot-assisted mechanical therapy attenuates stroke-induced limb skeletal muscle injury
    Authors: Chandan K Sen
    FASEB J, 2016;0(0):.  2016
  31. Tumor necrosis factor-Alpha stimulates cytokine expression and transient sensitization of trigeminal nociceptive neurons
    Authors: Paul L Durham
    Arch. Oral Biol., 2016;0(0):.  2016
  32. Nanoparticles Effectively Target Rapamycin Delivery to Sites of Experimental Aortic Aneurysm in Rats
    Authors: Takuro Shirasu
    PLoS ONE, 2016;11(6):e0157813.  2016
  33. Aberrant cytokine secretion and zinc uptake in chronic cadmium-exposed lung epithelial cells
    Authors: Andy T Y Lau
    Proteomics Clin Appl, 2016;0(0):.  2016
  34. FT011, a Novel Cardiorenal Protective Drug, Reduces Inflammation, Gliosis and Vascular Injury in Rats with Diabetic Retinopathy.
    Authors: Deliyanti D, Zhang Y, Khong F, Berka D, Stapleton D, Kelly D, Wilkinson-Berka J
    PLoS ONE, 2015;10(7):e0134392.  2015
  35. Mesenchymal stem cells from rats with chronic kidney disease exhibit premature senescence and loss of regenerative potential.
    Authors: Klinkhammer B, Kramann R, Mallau M, Makowska A, van Roeyen C, Rong S, Buecher E, Boor P, Kovacova K, Zok S, Denecke B, Stuettgen E, Otten S, Floege J, Kunter U
    PLoS ONE, 2014;9(3):e92115.  2014
  36. Novel mechanisms of sildenafil in pulmonary hypertension involving cytokines/chemokines, MAP kinases and Akt.
    Authors: Kiss T, Kovacs K, Komocsi A, Tornyos A, Zalan P, Sumegi B, Gallyas F, Kovacs K
    PLoS ONE, 2014;9(8):e104890.  2014
  37. Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.
    Authors: Altara, Raffaele, Manca, Marco, Hessel, Marleen, Janssen, Ben J, Struijker-Boudier, Harry H, Hermans, Rob J J, Blankesteijn, W Matthi
    BMC Biotechnol, 2014;14(0):63.  2014
  38. A RHAMM mimetic peptide blocks hyaluronan signaling and reduces inflammation and fibrogenesis in excisional skin wounds.
    Authors: Tolg C, Hamilton S, Zalinska E, McCulloch L, Amin R, Akentieva N, Winnik F, Savani R, Bagli D, Luyt L, Cowman M, McCarthy J, Turley E
    Am J Pathol, 2012;181(4):1250-70.  2012
  39. Human embryonic mesenchymal stem cell-derived conditioned medium rescues kidney function in rats with established chronic kidney disease.
    Authors: van Koppen A, Joles JA, van Balkom BW, Lim SK, de Kleijn D, Giles RH, Verhaar MC
    PLoS ONE, 2012;7(6):e38746.  2012
  40. Xanthine oxidoreductase promotes the inflammatory state of mononuclear phagocytes through effects on chemokine expression, peroxisome proliferator-activated receptor-gamma sumoylation, and HIF-1alpha.
    Authors: Gibbings S, Elkins ND, Fitzgerald H, Tiao J, Weyman ME, Shibao G, Fini MA, Wright RM
    J. Biol. Chem., 2011;286(2):961-75.  2011
  41. Treatment of experimental adjuvant arthritis with a novel folate receptor-targeted folic acid-aminopterin conjugate.
    Authors: Lu Y, Stinnette TW, Westrick E, Klein PJ, Gehrke MA, Cross VA, Vlahov IR, Low PS, Leamon CP
    Arthritis Res. Ther., 2011;13(2):R56.  2011
  42. A postweaning iron-adequate diet following neonatal iron deficiency affects iron homeostasis and growth in young rats.
    Authors: Hegde NV, Jensen GL, Unger EL
    J. Nutr., 2011;141(3):386-90.  2011
  43. Construction and characterization of a novel DNA vaccine that is potent antigen-specific tolerizing therapy for experimental arthritis by increasing CD4+CD25+Treg cells and inducing Th1 to Th2 shift in both cells and cytokines.
    Authors: Song X, Liang F, Liu N, Luo Y, Xue H, Yuan F, Tan L, Sun Y, Xi C, Xi Y
    Vaccine, 2009;27(5):690-700.  2009

FAQs

  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

View all Proteome Profiler Antibody Array FAQs

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Reviews for Proteome Profiler Rat Cytokine Array Kit, Panel A

Average Rating: 4.5 (Based on 8 Reviews)

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Proteome Profiler Rat Cytokine Array Kit, Panel A
By Anonymous on 03/11/2021
Proteome Profiler Rat Cytokine Array Kit, Panel A
By Reetu Singh on 11/15/2020
Proteome Profiler Rat Cytokine Array Kit, Panel A
By Anonymous on 05/15/2020

This kit is easy to use and would definitely use again.

Proteome Profiler Rat Cytokine Array Kit, Panel A ARY008
Proteome Profiler Rat Cytokine Array Kit, Panel A
By Anonymous on 02/01/2020

Performed well, great tool to screen for possible targets before making a more quantitative and more expensive assay. Spots can be easily evaluated using e.g., Bio-Rad ImageLab, and the semi-quantitative analysis can basically detect substantial differences between experimental groups.

Proteome Profiler Rat Cytokine Array Kit, Panel A ARY008
Proteome Profiler Rat Cytokine Array Kit, Panel A
By Leslie Priddy on 04/01/2018
Proteome Profiler Rat Cytokine Array Kit, Panel A
By Anonymous on 01/20/2017

This array kit is a very nice tool to screen potential cytokines in spent media of cell culture.

Proteome Profiler Rat Cytokine Array Kit, Panel A ARY008
Proteome Profiler Rat Cytokine Array Kit, Panel A
By Anonymous on 01/03/2017

This kit is an excellent tool in screening potential cytokines and chemokines. However, it would be nice if more targets can be included for this Rat cytokine panel (since the mouse and human panel has XL format).

Proteome Profiler Rat Cytokine Array Kit, Panel A
By Anonymous on 10/06/2016
Proteome Profiler Rat Cytokine Array Kit, Panel A ARY008