Proteome Profiler Rat Cytokine Array Kit, Panel A
Proteome Profiler Rat Cytokine Array Kit, Panel A Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected rat cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.
Troubleshooting GuideKey Benefits
- Detects 29 rat cytokines and chemokines simultaneously
- Requires no specialized equipment
- Compatible with LI-COR and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Rat Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
- Rectangular 4-Well Multi-dish
- 4 Rat Cytokine Array, Panel A nitrocellulose membranes spotted with 29 different antibodies to rat cytokines and chemokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Rat Cytokine Array, Panel A
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. | ||
| CXCL1/CINC-1 | IL-1ra/IL-1F3 | L-Selectin |
| CXCL3/CINC-2 alpha/beta | IL-2 | CXCL9/MIG |
| CXCL2/CINC-3 | IL-3 | CCL3/MIP-1 alpha |
| CNTF | IL-4 | CCL20/MIP-3 alpha |
| Fractalkine/CXC3CL1 | IIL-6 | CCL5/RANTES |
| GM-CSF | IL-10 | CXCL7/Thymus Chemokine |
| ICAM-1 | IL-13 | TIMP-1 |
| IFN-gamma | IL-17 | TNF-alpha |
| IL-1 alpha/IL-1F1 | CXCL10/IP-10 | VEGF |
| IL-1 beta/IL-1F2 | LIX | |
Assays for Analytes represented in the Rat Cytokine Array Kit, Panel A
| Analyte | Quantikine® ELISA Kits | DuoSet® ELISA Development Systems |
| CXCL1/CINC-1 | DY515 | |
| CXCL3/CINC-2 alpha/beta | RCN200 | DY516 |
| CXCL2/CINC-3 | RCN300 | DY525 |
| CNTF | DY557 | |
| Fractalkine/CX3CL1 | MCX310 | DY537 |
| GM-CSF | DY518 | |
| ICAM-1 | RIC100 | DY583 |
| IFN-gamma | RIF00 | DY585 |
| IL-1 alpha | RRA00 | DY500 |
| IL-1 beta | RLB00 | DY501 |
| IL-1ra | ||
| IL-2 | R2000 | DY502 |
| IL-3 | ||
| IL-4 | R4000 | DY504 |
| IL-6 | R6000B | DY506 |
| IL-10 | R1000 | DY522 |
| IL-13 | ||
| IL-17 | ||
| CXCL10/IP-10 | ||
| LIX | DY543 | |
| L-Selectin | DY1534 | |
| CXCL9/MIG | ||
| CCL3/MIP-1 alpha | ||
| CCL20/MIP-3 alpha | DY540 | |
| CCL5/RANTES | MMR00 | |
| CXCL7/Thymus Chemokine | DY1116 | |
| TIMP-1 | RTM100 | DY580 |
| TNF-alpha | RTA00 | DY510 |
| VEGF | RRV00 | DY564 |
Specifications
Product Datasheets
Data Example
The Rat Cytokine Array detects multiple analytes in cell culture supernates. NR8383 rat alveolar macrophage cells were either untreated or treated as below. Data shown are from a two minute exposure to X-ray film. A, untreated. B, Treated with 100 ng/mL LPS for 24 hours. C, Pre-treated with 100 nM Dexamethasone for 72 hours followed by treatment with 100 ng/mL LPS for 24 hours.
The Rat Cytokine Array detects multiple analytes in tissue lysates and serum. A rat was injected with 0.1 mg/kg LPS. After 24 hours, tissues were excised and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film (Panels A-B). Tissues were excised from untreated rats and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film (Panels C-E). Serum samples from 15 week old male rats were prepared as described in the Sample Collection and Storage section. 100 µL of serum was run on the array. Data shown are from a 5 minute exposure to X-ray film (Panel F).
The Rat Cytokine Array detects multiple analytes in cell culture supernates and lysates. (A. and B.) Supernates from NR8383 rat alveolar macrophage cell line untreated or treated with 50 ng/mL LPS for 24 hours. 500 μL of supernate was used for each array shown. (C. and D.) Lysates from NR8383 rat alveolar macrophage cell line untreated or treated with 50 ng/mL LPS for 24 hours. 200 μg of lysates was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
The Rat Cytokine Array detects cytokines present in serum and tissue. Representative membranes and selective quantification are shown for rat serum (A., 150 μL), lysates of rat lung tissue (B., 400 μg), and lysates of rat liver tissue (C., 400 μg). Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
Citations for Proteome Profiler Rat Cytokine Array Kit, Panel A
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Peripheral Macrophage-derived Exosomes promote repair after Spinal Cord Injury by inducing Local Anti-inflammatory type Microglial Polarization via Increasing Autophagy
Authors: B Zhang, F Lin, J Dong, J Liu, Z Ding, J Xu
International journal of biological sciences, 2021;17(5):1339-1352. 2021
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Beneficial effects of IL-4 and IL-6 on rat neonatal target cardiac cells
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Sci Rep, 2020;10(1):12350. 2020
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Recombinant Alkaline Phosphatase Prevents Acute on Chronic Liver Failure
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Authors: P Basu, RS Hornung, DL Averitt, C Maier
Oxid Med Cell Longev, 2019;2019(0):8594375. 2019
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The effects of human immunoglobulin G on enhancing tissue protection and neurobehavioral recovery after traumatic cervical spinal cord injury are mediated through the neurovascular unit
Authors: JCT Chio, J Wang, A Badner, J Hong, V Surendran, MG Fehlings
J Neuroinflammation, 2019;16(1):141. 2019
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Localized Intrathecal Delivery of Mesenchymal Stromal Cells Conditioned Medium Improves Functional Recovery in a Rat Model of Spinal Cord Injury
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EBI2 regulates pro-inflammatory signalling and cytokine release in astrocytes
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Sphingosine 1-phosphate receptors regulate TLR4-induced CXCL5 release from astrocytes and microglia
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Authors: N Gupta, A Sahu, A Prabhakar, T Chatterjee, T Tyagi, B Kumari, N Khan, V Nair, N Bajaj, M Sharma, MZ Ashraf
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Nature, 2017;541(7638):481-487. 2017
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Human embryonic mesenchymal stem cell-derived conditioned medium rescues kidney function in rats with established chronic kidney disease.
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FAQs
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Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
Reviews for Proteome Profiler Rat Cytokine Array Kit, Panel A
Average Rating: 4.5 (Based on 8 Reviews)
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This kit is easy to use and would definitely use again.
Performed well, great tool to screen for possible targets before making a more quantitative and more expensive assay. Spots can be easily evaluated using e.g., Bio-Rad ImageLab, and the semi-quantitative analysis can basically detect substantial differences between experimental groups.
This array kit is a very nice tool to screen potential cytokines in spent media of cell culture.
This kit is an excellent tool in screening potential cytokines and chemokines. However, it would be nice if more targets can be included for this Rat cytokine panel (since the mouse and human panel has XL format).
