Proteome Profiler Rat Cytokine Array Kit, Panel A

Contains 4 membranes - each spotted in duplicate with 29 different cytokine antibodies
Catalog # Availability Size / Price Qty
ARY008
Product Details
Citations (31)
FAQs
Supplemental Products
Reviews (4)

Proteome Profiler Rat Cytokine Array Kit, Panel A Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected rat cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.

Troubleshooting Guide

Key Benefits

  • Detects 29 rat cytokines and chemokines simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR and chemiluminescence detection
 

Principle of the Assay

The Proteome Profiler Rat Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 
Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Rat Cytokine Array, Panel A nitrocellulose membranes spotted with 29 different antibodies to rat cytokines and chemokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Rat Cytokine Array, Panel A
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

 

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
CXCL1/CINC-1 IL-1ra/IL-1F3 L-Selectin
CXCL3/CINC-2 alpha/beta IL-2 CXCL9/MIG
CXCL2/CINC-3 IL-3 CCL3/MIP-1 alpha
CNTF IL-4 CCL20/MIP-3 alpha
Fractalkine/CXC3CL1 IIL-6 CCL5/RANTES
GM-CSF IL-10 CXCL7/Thymus Chemokine
ICAM-1 IL-13 TIMP-1
IFN-gamma IL-17 TNF-alpha
IL-1 alpha/IL-1F1 CXCL10/IP-10 VEGF
IL-1 beta/IL-1F2 LIX  

 

Assays for Analytes represented in the Rat Cytokine Array Kit, Panel A

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CXCL1/CINC-1

RCN100

DY515
CXCL3/CINC-2 alpha/beta RCN200 DY516
CXCL2/CINC-3 RCN300 DY525
CNTF   DY557
Fractalkine/CX3CL1 MCX310 DY537
GM-CSF   DY518
ICAM-1 RIC100 DY583
IFN-gamma RIF00 DY585
IL-1 alpha RRA00 DY500
IL-1 beta RLB00 DY501
IL-1ra    
IL-2 R2000 DY502
IL-3    
IL-4 R4000 DY504
IL-6 R6000B DY506
IL-10 R1000 DY522
IL-13    
IL-17    
CXCL10/IP-10    
LIX   DY543
L-Selectin   DY1534
CXCL9/MIG    
CCL3/MIP-1 alpha    
CCL20/MIP-3 alpha   DY540
CCL5/RANTES MMR00  
CXCL7/Thymus Chemokine   DY1116
TIMP-1 RTM100 DY580
TNF-alpha RTA00 DY510
VEGF RRV00 DY564

 

 

Data Examples
The Rat Cytokine Array detects multiple analytes in cell culture supernates.
View Larger Image

Figure 1. The Rat Cytokine Array detects multiple analytes in cell culture supernates. NR8383 rat alveolar macrophage cells were either untreated or treated as below. Data shown are from a two minute exposure to X-ray film. A, untreated. B, Treated with 100 ng/mL LPS for 24 hours. C, Pre-treated with 100 nM Dexamethasone for 72 hours followed by treatment with 100 ng/mL LPS for 24 hours.

The Rat Cytokine Array detects multiple analytes in tissue lysates and serum
View Larger Image

Figure 2. The Rat Cytokine Array detects multiple analytes in tissue lysates and serum. A-B, A rat was injected with 0.1 mg/kg LPS. After 24 hours, tissues were excised and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film. C-E, Tissues were excised from untreated rats and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film. F, Serum samples from 15 week old male rats were prepared as described in the Sample Collection and Storage section. 100 µL of serum was run on the array. Data shown are from a 5 minute exposure to X-ray film.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Rat

Product Datasheets

Background: Multiplex Cytokine Assay Kits

Alternate Names
Multiplex Cytokine Assay Kits

Reagents Provided

Other Reagents Provided

Citations for Proteome Profiler Rat Cytokine Array Kit, Panel A

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. The effects of human immunoglobulin G on enhancing tissue protection and neurobehavioral recovery after traumatic cervical spinal cord injury are mediated through the neurovascular unit
    Authors: JCT Chio, J Wang, A Badner, J Hong, V Surendran, MG Fehlings
    J Neuroinflammation, 2019;16(1):141.  2019
  2. Dendrobium huoshanense polysaccharide regulates intestinal lamina propria immune response by stimulation of intestinal epithelial cells via toll-like receptor 4
    Authors: SZ Xie, ZZ Shang, QM Li, XQ Zha, LH Pan, JP Luo
    Carbohydr Polym, 2019;222(0):115028.  2019
  3. CGRP Induces Differential Regulation of Cytokines from Satellite Glial Cells in Trigeminal Ganglia and Orofacial Nociception
    Authors: S Afroz, R Arakaki, T Iwasa, M Oshima, M Hosoki, M Inoue, O Baba, Y Okayama, Y Matsuka
    Int J Mol Sci, 2019;20(3):.  2019
  4. EBI2 regulates pro-inflammatory signalling and cytokine release in astrocytes
    Authors: A Rutkowska, DR Shimshek, AW Sailer, KK Dev
    Neuropharmacology, 2018;133(0):121-128.  2018
  5. Paclitaxel�treatment and PC1/3 knockdown in macrophages is a promising anti-glioma strategy as revealed by proteomics and cytotoxicity studies
    Authors: M Duhamel, M Rose, F Rodet, AN Murgoci, L Zografidou, A Régnier-Vi, F Vanden Abe, F Kobeissy, S Nataf, L Pays, M Wisztorski, D Cizkova, I Fournier, M Salzet
    Mol. Cell Proteomics, 2018;0(0):.  2018
  6. Sphingosine 1-phosphate receptors regulate TLR4-induced CXCL5 release from astrocytes and microglia
    Authors: SA O'Sullivan, C O'Sullivan, LM Healy, KK Dev, GK Sheridan
    J. Neurochem., 2018;0(0):.  2018
  7. Localized Intrathecal Delivery of Mesenchymal Stromal Cells Conditioned Medium Improves Functional Recovery in a Rat Model of Spinal Cord Injury
    Authors: D Cizkova, V Cubinkova, T Smolek, AN Murgoci, J Danko, K Vdoviakova, F Humenik, M Cizek, J Quanico, I Fournier, M Salzet
    Int J Mol Sci, 2018;19(3):.  2018
  8. Aged blood factors decrease cellular responses associated with delayed gingival wound repair
    Authors: MP Saldías, C Fernández, A Morgan, C Díaz, D Morales, F Jaña, A Gómez, A Silva, F Briceño, A Oyarzún, F Maldonado, O Cerda, PC Smith, M Cáceres
    PLoS ONE, 2017;12(9):e0184189.  2017
  9. Acute Mobilization and Migration of Bone Marrow-derived Stem Cells following Anterior Cruciate Ligament Rupture
    Authors: T Maerz, M Fleischer, MD Newton, A Davidson, M Salisbury, P Altman, MD Kurdziel, K Anderson, A Bedi, KC Baker
    Osteoarthr. Cartil, 2017;0(0):.  2017
  10. Activation of NLRP3 inflammasome complex potentiates venous thrombosis in response to hypoxia
    Authors: N Gupta, A Sahu, A Prabhakar, T Chatterjee, T Tyagi, B Kumari, N Khan, V Nair, N Bajaj, M Sharma, MZ Ashraf
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.  2017
  11. Implication of Free Fatty Acids in Thrombin Generation and Fibrinolysis in Vascular Inflammation in Zucker Rats and Evolution with Aging
    Authors: J Lagrange, M Didelot, A Mohamadi, LA Walton, S Bloemen, B de Laat, H Louis, SN Thornton, B Derby, MJ Sherratt, B Fève, P Challande, R Akhtar, JK Cruickshan, P Lacolley, V Regnault
    Front Physiol, 2017;8(0):949.  2017
  12. Immune tuning scaffold for the local induction of a pro-regenerative environment
    Authors: B Corradetti, F Taraballi, C Corbo, F Cabrera, L Pandolfi, S Minardi, X Wang, J Van Eps, G Bauza, B Weiner, E Tasciotti
    Sci Rep, 2017;7(1):17030.  2017
  13. Adaptor protein-3: A key player in RBL-2H3 mast cell mediator release
    Authors: EZ da Silva, EG Freitas-Fi, DA de Souza-J, LL daSilva, MC Jamur, C Oliver
    PLoS ONE, 2017;12(3):e0173462.  2017
  14. Neurotoxic reactive astrocytes are induced by activated microglia
    Authors: SA Liddelow, KA Guttenplan, LE Clarke, FC Bennett, CJ Bohlen, L Schirmer, ML Bennett, AE Mnch, WS Chung, TC Peterson, DK Wilton, A Frouin, BA Napier, N Panicker, M Kumar, MS Buckwalter, DH Rowitch, VL Dawson, TM Dawson, B Stevens, BA Barres
    Nature, 2017;541(7638):481-487.  2017
  15. Cytokine evaluation in untreated and radioimmunotherapy-treated tumors in an immunocompetent rat model
    Authors: E Elgström, TG Ohlsson, SE Eriksson
    Tumour Biol., 2017;39(4):1010428317697.  2017
  16. Tumor necrosis factor-Alpha stimulates cytokine expression and transient sensitization of trigeminal nociceptive neurons
    Authors: Paul L Durham
    Arch. Oral Biol., 2016;0(0):.  2016
  17. Nanoparticles Effectively Target Rapamycin Delivery to Sites of Experimental Aortic Aneurysm in Rats
    Authors: Takuro Shirasu
    PLoS ONE, 2016;11(6):e0157813.  2016
  18. Myelin phagocytosis by astrocytes after myelin damage promotes lesion pathology
    Authors: Gerald Ponath
    Brain, 2016;0(0):.  2016
  19. Ocular Delivery of PACAP1-27 Protects the Retina From Ischemic Damage in Rodents
    Invest. Ophthalmol. Vis. Sci, 2016;57(15):6683-6691.  2016
  20. Aberrant cytokine secretion and zinc uptake in chronic cadmium-exposed lung epithelial cells
    Authors: Andy T Y Lau
    Proteomics Clin Appl, 2016;0(0):.  2016
  21. Robot-assisted mechanical therapy attenuates stroke-induced limb skeletal muscle injury
    Authors: Chandan K Sen
    FASEB J, 2016;0(0):.  2016
  22. FT011, a Novel Cardiorenal Protective Drug, Reduces Inflammation, Gliosis and Vascular Injury in Rats with Diabetic Retinopathy.
    Authors: Deliyanti D, Zhang Y, Khong F, Berka D, Stapleton D, Kelly D, Wilkinson-Berka J
    PLoS ONE, 2015;10(7):e0134392.  2015
  23. Novel mechanisms of sildenafil in pulmonary hypertension involving cytokines/chemokines, MAP kinases and Akt.
    Authors: Kiss T, Kovacs K, Komocsi A, Tornyos A, Zalan P, Sumegi B, Gallyas F, Kovacs K
    PLoS ONE, 2014;9(8):e104890.  2014
  24. Mesenchymal stem cells from rats with chronic kidney disease exhibit premature senescence and loss of regenerative potential.
    Authors: Klinkhammer B, Kramann R, Mallau M, Makowska A, van Roeyen C, Rong S, Buecher E, Boor P, Kovacova K, Zok S, Denecke B, Stuettgen E, Otten S, Floege J, Kunter U
    PLoS ONE, 2014;9(3):e92115.  2014
  25. Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.
    Authors: Altara, Raffaele, Manca, Marco, Hessel, Marleen, Janssen, Ben J, Struijker-Boudier, Harry H, Hermans, Rob J J, Blankesteijn, W Matthi
    BMC Biotechnol, 2014;14(0):63.  2014
  26. Human embryonic mesenchymal stem cell-derived conditioned medium rescues kidney function in rats with established chronic kidney disease.
    Authors: van Koppen A, Joles JA, van Balkom BW, Lim SK, de Kleijn D, Giles RH, Verhaar MC
    PLoS ONE, 2012;7(6):e38746.  2012
  27. A RHAMM mimetic peptide blocks hyaluronan signaling and reduces inflammation and fibrogenesis in excisional skin wounds.
    Authors: Tolg C, Hamilton S, Zalinska E, McCulloch L, Amin R, Akentieva N, Winnik F, Savani R, Bagli D, Luyt L, Cowman M, McCarthy J, Turley E
    Am J Pathol, 2012;181(4):1250-70.  2012
  28. Treatment of experimental adjuvant arthritis with a novel folate receptor-targeted folic acid-aminopterin conjugate.
    Authors: Lu Y, Stinnette TW, Westrick E, Klein PJ, Gehrke MA, Cross VA, Vlahov IR, Low PS, Leamon CP
    Arthritis Res. Ther., 2011;13(2):R56.  2011
  29. A postweaning iron-adequate diet following neonatal iron deficiency affects iron homeostasis and growth in young rats.
    Authors: Hegde NV, Jensen GL, Unger EL
    J. Nutr., 2011;141(3):386-90.  2011
  30. Xanthine oxidoreductase promotes the inflammatory state of mononuclear phagocytes through effects on chemokine expression, peroxisome proliferator-activated receptor-gamma sumoylation, and HIF-1alpha.
    Authors: Gibbings S, Elkins ND, Fitzgerald H, Tiao J, Weyman ME, Shibao G, Fini MA, Wright RM
    J. Biol. Chem., 2011;286(2):961-75.  2011
  31. Construction and characterization of a novel DNA vaccine that is potent antigen-specific tolerizing therapy for experimental arthritis by increasing CD4+CD25+Treg cells and inducing Th1 to Th2 shift in both cells and cytokines.
    Authors: Song X, Liang F, Liu N, Luo Y, Xue H, Yuan F, Tan L, Sun Y, Xi C, Xi Y
    Vaccine, 2009;27(5):690-700.  2009

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Proteome Profiler Rat Cytokine Array Kit, Panel A
By Anonymous on 04/01/2018

Proteome Profiler Rat Cytokine Array Kit, Panel A
By Anonymous on 01/20/2017

This array kit is a very nice tool to screen potential cytokines in spent media of cell culture.


Proteome Profiler Rat Cytokine Array Kit, Panel A
By Anonymous on 01/03/2017

This kit is an excellent tool in screening potential cytokines and chemokines. However, it would be nice if more targets can be included for this Rat cytokine panel (since the mouse and human panel has XL format).


Proteome Profiler Rat Cytokine Array Kit, Panel A
By Anonymous on 10/06/2016