Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected rat cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.

Key Benefits

  • Detects 29 rat cytokines and chemokines simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR and chemiluminescence detection
 

Principle of the Assay

The Proteome Profiler Rat Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 
Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Rat Cytokine Array, Panel A nitrocellulose membranes spotted with 29 different antibodies to rat cytokines and chemokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Rat Cytokine Array, Panel A
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

 

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
CXCL1/CINC-1 IL-1ra/IL-1F3 L-Selectin
CXCL3/CINC-2 alpha/beta IL-2 CXCL9/MIG
CXCL2/CINC-3 IL-3 CCL3/MIP-1 alpha
CNTF IL-4 CCL20/MIP-3 alpha
Fractalkine/CXC3CL1 IIL-6 CCL5/RANTES
GM-CSF IL-10 CXCL7/Thymus Chemokine
ICAM-1 IL-13 TIMP-1
IFN-gamma IL-17 TNF-alpha
IL-1 alpha/IL-1F1 CXCL10/IP-10 VEGF
IL-1 beta/IL-1F2 LIX  

 

Assays for Analytes represented in the Rat Cytokine Array Kit, Panel A

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CXCL1/CINC-1

RCN100

DY515
CXCL3/CINC-2 alpha/beta RCN200 DY516
CXCL2/CINC-3 RCN300 DY525
CNTF   DY557
Fractalkine/CX3CL1 MCX310 DY537
GM-CSF   DY518
ICAM-1 RIC100 DY583
IFN-gamma RIF00 DY585
IL-1 alpha RRA00 DY500
IL-1 beta RLB00 DY501
IL-1ra    
IL-2 R2000 DY502
IL-3    
IL-4 R4000 DY504
IL-6 R6000B DY506
IL-10 R1000 DY522
IL-13    
IL-17    
CXCL10/IP-10    
LIX   DY543
L-Selectin   DY1534
CXCL9/MIG    
CCL3/MIP-1 alpha    
CCL20/MIP-3 alpha   DY540
CCL5/RANTES MMR00  
CXCL7/Thymus Chemokine   DY1116
TIMP-1 RTM100 DY580
TNF-alpha RTA00 DY510
VEGF RRV00 DY564

 

 
Data Examples
The Rat Cytokine Array detects multiple analytes in cell culture supernates.
View Larger Image

Figure 1. The Rat Cytokine Array detects multiple analytes in cell culture supernates. NR8383 rat alveolar macrophage cells were either untreated or treated as below. Data shown are from a two minute exposure to X-ray film. A, untreated. B, Treated with 100 ng/mL LPS for 24 hours. C, Pre-treated with 100 nM Dexamethasone for 72 hours followed by treatment with 100 ng/mL LPS for 24 hours.

The Rat Cytokine Array detects multiple analytes in tissue lysates and serum
View Larger Image

Figure 2. The Rat Cytokine Array detects multiple analytes in tissue lysates and serum. A-B, A rat was injected with 0.1 mg/kg LPS. After 24 hours, tissues were excised and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film. C-E, Tissues were excised from untreated rats and prepared as described in the Sample Collection and Storage section. 400 µg of lysate was run on each array. Data shown are from a 7 minute exposure to X-ray film. F, Serum samples from 15 week old male rats were prepared as described in the Sample Collection and Storage section. 100 µL of serum was run on the array. Data shown are from a 5 minute exposure to X-ray film.

Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. Neurotoxic reactive astrocytes are induced by activated microglia
    Authors: SA Liddelow, KA Guttenplan, LE Clarke, FC Bennett, CJ Bohlen, L Schirmer, ML Bennett, AE Mnch, WS Chung, TC Peterson, DK Wilton, A Frouin, BA Napier, N Panicker, M Kumar, MS Buckwalter, DH Rowitch, VL Dawson, TM Dawson, B Stevens, BA Barres
    Nature, 2017;541(7638):481-487. 2017
  2. Adaptor protein-3: A key player in RBL-2H3 mast cell mediator release
    Authors: EZ da Silva, EG Freitas-Fi, DA de Souza-J, LL daSilva, MC Jamur, C Oliver
    PLoS ONE, 2017;12(3):e0173462. 2017
  3. Activation of NLRP3 inflammasome complex potentiates venous thrombosis in response to hypoxia
    Authors: N Gupta, A Sahu, A Prabhakar, T Chatterjee, T Tyagi, B Kumari, N Khan, V Nair, N Bajaj, M Sharma, MZ Ashraf
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017
  4. Ocular Delivery of PACAP1-27 Protects the Retina From Ischemic Damage in Rodents
    Invest. Ophthalmol. Vis. Sci, 2016;57(15):6683-6691. 2016
  5. Nanoparticles Effectively Target Rapamycin Delivery to Sites of Experimental Aortic Aneurysm in Rats
    Authors: Takuro Shirasu
    PLoS ONE, 2016;11(6):e0157813. 2016
  6. FT011, a Novel Cardiorenal Protective Drug, Reduces Inflammation, Gliosis and Vascular Injury in Rats with Diabetic Retinopathy.
    Authors: Deliyanti D, Zhang Y, Khong F, Berka D, Stapleton D, Kelly D, Wilkinson-Berka J
    PLoS ONE, 2015;10(7):e0134392. 2015
  7. Mesenchymal stem cells from rats with chronic kidney disease exhibit premature senescence and loss of regenerative potential.
    Authors: Klinkhammer B, Kramann R, Mallau M, Makowska A, van Roeyen C, Rong S, Buecher E, Boor P, Kovacova K, Zok S, Denecke B, Stuettgen E, Otten S, Floege J, Kunter U
    PLoS ONE, 2014;9(3):e92115. 2014
  8. Novel mechanisms of sildenafil in pulmonary hypertension involving cytokines/chemokines, MAP kinases and Akt.
    Authors: Kiss T, Kovacs K, Komocsi A, Tornyos A, Zalan P, Sumegi B, Gallyas F, Kovacs K
    PLoS ONE, 2014;9(8):e104890. 2014
  9. Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.
    Authors: Altara, Raffaele, Manca, Marco, Hessel, Marleen, Janssen, Ben J, Struijker-Boudier, Harry H, Hermans, Rob J J, Blankesteijn, W Matthi
    BMC Biotechnol, 2014;14(0):63. 2014
  10. A RHAMM mimetic peptide blocks hyaluronan signaling and reduces inflammation and fibrogenesis in excisional skin wounds.
    Authors: Tolg C, Hamilton S, Zalinska E, McCulloch L, Amin R, Akentieva N, Winnik F, Savani R, Bagli D, Luyt L, Cowman M, McCarthy J, Turley E
    Am J Pathol, 2012;181(4):1250-70. 2012
  11. Human embryonic mesenchymal stem cell-derived conditioned medium rescues kidney function in rats with established chronic kidney disease.
    Authors: van Koppen A, Joles JA, van Balkom BW, Lim SK, de Kleijn D, Giles RH, Verhaar MC
    PLoS ONE, 2012;7(6):e38746. 2012
  12. Xanthine oxidoreductase promotes the inflammatory state of mononuclear phagocytes through effects on chemokine expression, peroxisome proliferator-activated receptor-gamma sumoylation, and HIF-1alpha.
    Authors: Gibbings S, Elkins ND, Fitzgerald H, Tiao J, Weyman ME, Shibao G, Fini MA, Wright RM
    J. Biol. Chem., 2011;286(2):961-75. 2011
  13. A postweaning iron-adequate diet following neonatal iron deficiency affects iron homeostasis and growth in young rats.
    Authors: Hegde NV, Jensen GL, Unger EL
    J. Nutr., 2011;141(3):386-90. 2011
  14. Treatment of experimental adjuvant arthritis with a novel folate receptor-targeted folic acid-aminopterin conjugate.
    Authors: Lu Y, Stinnette TW, Westrick E, Klein PJ, Gehrke MA, Cross VA, Vlahov IR, Low PS, Leamon CP
    Arthritis Res. Ther., 2011;13(2):R56. 2011
  15. Construction and characterization of a novel DNA vaccine that is potent antigen-specific tolerizing therapy for experimental arthritis by increasing CD4+CD25+Treg cells and inducing Th1 to Th2 shift in both cells and cytokines.
    Authors: Song X, Liang F, Liu N, Luo Y, Xue H, Yuan F, Tan L, Sun Y, Xi C, Xi Y
    Vaccine, 2009;27(5):690-700. 2009
Expand to show all 15 Citations
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Average Rating: 5 (Based on 3 reviews)

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 Proteome Profiler Rat Cytokine Array Kit, Panel A ARY008
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  Anonymous 01/20/2017
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 Proteome Profiler Rat Cytokine Array Kit, Panel A ARY008
: Proteome Profiler Rat Cytokine Array Kit, Panel A [ARY008]

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Other Experimental DetailsThis array kit is a very nice tool to screen potential cytokines in spent media of cell culture.
  Excellent
  Anonymous 01/03/2017
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Other Experimental Details

Other Experimental DetailsThis kit is an excellent tool in screening potential cytokines and chemokines. However, it would be nice if more targets can be included for this Rat cytokine panel (since the mouse and human panel has XL format).
 Proteome Profiler Rat Cytokine Array Kit, Panel A ARY008
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Excellent
  Anonymous 10/06/2016
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 Proteome Profiler Rat Cytokine Array Kit, Panel A ARY008
: Proteome Profiler Rat Cytokine Array Kit, Panel A [ARY008]

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