R-Spondin 1 Antibody - BSA Free

Name: R-Spondin 1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-77354
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-77354

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Tagged Protein Expression

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human R-Spondin 1. The immunogen is located within the first 50 amino acids of R-Spondin 1. Amino Acid Sequence: RGIKGKRQRRISAEGS

Specificity

At least three isoforms of R-Spondin 1 are known to exist; this antibody will detect the largest and smallest isoforms. R-Spondin 1 antibody will not cross-react with other RSPO family members. Human R-Spondin 1 has 3 isoforms, including isoform 1 (263aa, 29kD), isoform 2 (236aa, 26kD), and isoform 3 (200aa, 22kD). Mouse R-Spondin 1 had 1 isoform (265aa, 29kD) and rat R-Spondin 1 had 1 isoform (262aa, 29kD). NBP1-77354 can detect human, mouse and rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for R-Spondin 1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: R-Spondin 1 Antibody - BSA Free [NBP1-77354] -

Immunocytochemistry/ Immunofluorescence: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Spleen. Immunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling R-Spondin 1 with at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) with Phylloidin staining (red) and DAPI staining (blue).

Western Blot: R-Spondin 1 AntibodyBSA Free [NBP1-77354]

Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 15 ug of spleen lysates per lane. Antibodies: RSPO1,5171 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL), 1h incubationat RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgGHRP conjugate at 1:10000 dilution.

Western Blot: R-Spondin 1 AntibodyBSA Free [NBP1-77354]

Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Analysis in human heart tissue lysate with antibody at (A) 1 and (B) 2 ug/mL.

Western Blot: R-Spondin 1 AntibodyBSA Free [NBP1-77354]

Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 30 ng of lysates per lane. Lane 1: 0.5ug/ml, Lane 2: 1ug/ml

Western Blot: R-Spondin 1 AntibodyBSA Free [NBP1-77354]

Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 30 ng of human RSPO1 recombinant protein perlane. Antibodies: RSPO1, 5171 (Lane 1: 0.5 ug/mL, Lane 2:1 ug/mL, Lane 3: 2 ug/mL), 1h incubation at RT in 5%NFDM/TBST. Secondary: Goat anti-rabbit IgG HRPconjugate at 1:10000 dilution.

Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354] -

Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Heart. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-R-Spondin 1 antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354] -

Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-R-Spondin 1 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354] -

Immunohistochemistry: R-Spondin 1 Antibody - BSA Free [NBP1-77354] - Validation of R-Spondin 1 in Human Spleen Tissue. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-R-Spondin 1 antibody at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

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Applications for R-Spondin 1 Antibody - BSA Free

Application

Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Immunohistochemistry

2.5-5 ug/mL

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

0.5-2 ug/mL

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: R-Spondin 1

RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels. At least two isoforms of RSPO1 are known to exist. RSPO1 antibody will not cross-react with RSPO family members.

Long Name

Roof Plate-specific Spondin 1

Alternate Names

Cristin 3, CRISTIN3, HRspo1, RSPO, RSPO1, RSpondin 1

Gene Symbol

RSPO1

UniProt

NP_001033722 (Human)

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Product Specific Notices for R-Spondin 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.