Recognizes an epitope in the cytoplasmic portion of rat Glut4 and has been shown to bind only to the muscle-adipose isoform of the glucose transporter, which is antigenically unique from the other transport proteins (1-3). Recognizes Glut4 in human, monkey, rat, mouse, and rabbit systems. It does not recognize this protein in canine systems. Its ability to bind to glucose transporter from other species has not been tested.
Monoclonal Mouse IgG1 Clone # 1F8
Protein A or G purified from hybridoma culture supernatant
Partially purified vesicles containing Glut4
Supplied as a 0.2 um filtered solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Fischer, et al. (1997) J. Biol. Chem. 272:7085.
Membrane vesicles from fat and muscle were immunoadsorbed using this antibody linked to sepharose (3, 4).
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Glut4 in L6 Rat Cell Line. Glut4 was detected in immersion fixed L6 rat myoblast cell line (differentiated to muscle) using 10 µg/mL Rat Glut4 Monoclonal Antibody (Catalog # MAB1262) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
Glut4 belongs to the facilitative glucose transporter protein family
that comprises 13 members. It is an integral membrane protein with 12
transmembrane domains. Glut4 is expressed in the insulin-responsive
tissues, muscle and adipose tissue. Insulin stimulation leads to
translocation of Glut4 from intracellular stores to the cell surface
resulting in the enhancement of glucose uptake.
James, et al. (1988) Nature 333:183.
Fukumoto, et al. (1989) J. Biol. Chem. 264:7776.
Zorzano, et al. (1989) J. Biol. Chem. 264:12358.
Rodnick, et al. (1992) J. Biol. Chem. 267:6278.
Entrez Gene IDs:
6517 (Human); 20528 (Mouse); 25139 (Rat)
GLUT-4; GLUT4Glucose transporter type 4, insulin-responsive; insulin-responsive glucose transporter type 4; SLC2A4; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2, facilitated glucose transporter member 4
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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