Rat Glut4 Antibody

(10 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Recognizes an epitope in the cytoplasmic portion of rat Glut4 and has been shown to bind only to the muscle-adipose isoform of the glucose transporter, which is antigenically unique from the other transport proteins (1-3). Recognizes Glut4 in human, monkey, rat, mouse, and rabbit systems. It does not recognize this protein in canine systems. Its ability to bind to glucose transporter from other species has not been tested.
  • Source
    Monoclonal Mouse IgG1 Clone # 1F8
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Partially purified vesicles containing Glut4
  • Formulation
    Supplied as a 0.2 um filtered solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    Zorzano, et al. (1989) J. Biol. Chem. 264:12358.
  • Immunoprecipitation
    Fischer, et al. (1997) J. Biol. Chem. 272:7085.
  • Immunoaffinity Purification
    Membrane vesicles from fat and muscle were immunoadsorbed using this antibody linked to sepharose (3, 4).
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Glut4 in L6 Rat Cell Line. Glut4 was detected in immersion fixed L6 rat myoblast cell line (differentiated to muscle) using 10 µg/mL Rat Glut4 Monoclonal Antibody (Catalog # MAB1262) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Shipping
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Glut4
Glut4 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut4 is expressed in the insulin-responsive tissues, muscle and adipose tissue. Insulin stimulation leads to translocation of Glut4 from intracellular stores to the cell surface resulting in the enhancement of glucose uptake.
  • References:
    1. James, et al. (1988) Nature 333:183.
    2. Fukumoto, et al. (1989) J. Biol. Chem. 264:7776.
    3. Zorzano, et al. (1989) J. Biol. Chem. 264:12358.
    4. Rodnick, et al. (1992) J. Biol. Chem. 267:6278.
  • Entrez Gene IDs:
    6517 (Human); 20528 (Mouse); 25139 (Rat)
  • Alternate Names:
    Glut4; GLUT-4; GLUT4Glucose transporter type 4, insulin-responsive; insulin-responsive glucose transporter type 4; SLC2A4; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2, facilitated glucose transporter member 4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. HDL and Glut1 inhibition reverse a hypermetabolic state in mouse models of myeloproliferative disorders.
    Authors: Gautier E, Westerterp M, Bhagwat N, Cremers S, Shih A, Abdel-Wahab O, Lutjohann D, Randolph G, Levine R, Tall A, Yvan-Charvet L
    J Exp Med, 2013;210(2):339-53.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. E4orf1: a novel ligand that improves glucose disposal in cell culture.
    Authors: Dhurandhar EJ, Dubuisson O, Mashtalir N
    PLoS ONE, 2011;6(8):e23394.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Nuclear factor-kappaB decoy oligodeoxynucleotides ameliorate impaired glucose tolerance and insulin resistance in mice with cecal ligation and puncture-induced sepsis.
    Authors: Matsuda N, Yamamoto S, Yokoo H, Tobe K, Hattori Y
    Crit. Care Med., 2009;37(10):2791-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. DOC2B: a novel syntaxin-4 binding protein mediating insulin-regulated GLUT4 vesicle fusion in adipocytes.
    Authors: Fukuda N, Emoto M, Nakamori Y, Taguchi A, Miyamoto S, Uraki S, Oka Y, Tanizawa Y
    Diabetes, 2009;58(2):377-84.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  5. A transgenic mouse model to study glucose transporter 4myc regulation in skeletal muscle.
    Authors: Schertzer JD, Antonescu CN, Bilan PJ, Jain S, Huang X, Liu Z, Bonen A, Klip A
    Endocrinology, 2009;150(4):1935-40.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IP
  6. Deficiency and inhibition of cathepsin K reduce body weight gain and increase glucose metabolism in mice.
    Authors: Yang M, Sun J, Zhang T, Liu J, Zhang J, Shi MA, Darakhshan F, Guerre-Millo M, Clement K, Gelb BD, Dolgnov G, Shi GP
    Arterioscler. Thromb. Vasc. Biol., 2008;28(12):2202-8.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  7. The human glomerular podocyte is a novel target for insulin action.
    Authors: Coward RJ, Welsh GI, Yang J, Tasman C, Lennon R, Koziell A, Satchell S, Holman GD, Kerjaschki D, Tavare JM, Mathieson PW, Saleem MA
    Diabetes, 2005;54(11):3095-102.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. Effects of hormone therapy on insulin signaling proteins in skeletal muscle of cynomolgus monkeys.
    Authors: Shadoan MK, Zhang L, Wagner JD
    Steroids, 2004;69(5):313-8.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Type: Tissue Homogenates
    Application: WB
  9. Ischemic preconditioning activates AMPK in a PKC-dependent manner and induces GLUT4 up-regulation in the late phase of cardioprotection.
    Authors: Nishino Y, Miura T, Miki T, Sakamoto J, Nakamura Y, Ikeda Y, Kobayashi H, Shimamoto K
    Cardiovasc. Res., 2004;61(3):610-9.
    Species: Rabbit
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. Requirement for 3-phosphoinositide-kependent dinase-1 (PDK-1) in insulin-induced glucose uptake in immortalized brown adipocytes.
    Authors: Sakaue H, Nishizawa A, Ogawa W, Teshigawara K, Mori T, Takashima Y, Noda T, Kasuga M
    J. Biol. Chem., 2003;278(40):38870-4.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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