Rat IgG HRP-conjugated Antibody

Catalog # Availability Size / Price Qty
HAF005
Detection of Rat IgGPrimary Antibody by Western Blot
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Product Details
Citations (8)
FAQs
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Rat IgG HRP-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IgG1, IgG2A, IgG2B heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with human IgG and rabbit IgG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rat IgG
Formulation
Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
Label
HRP (Horseradish Peroxidase)

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
 
Simple Western
Use at a 1:50 dilution.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Rat IgGPrimary Antibody by Western Blot View Larger

Detection of Rat IgGPrimary Antibody by Western Blot Western blot shows detection of Rat Anti-Human/Mouse Wnt-5a MonoclonalAntibody (Catalog # MAB645)using a 1:1000 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody
(Catalog # HAF005).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.

Simple Western Detection of Rat IgG Primary Antibody by Simple WesternTM. View Larger

Detection of Rat IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of RatAnti-Mouse S100A9 Monoclonal Antibody
(Catalog #MAB2065)using a 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions andusing the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

Product Datasheets

Citations for Rat IgG HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Regulation of vitamin D metabolizing enzymes in murine renal and extrarenal tissues by dietary phosphate, FGF23, and 1,25(OH)2D3
    Authors: L Kägi, C Bettoni, EM Pastor-Arr, U Schnitzbau, N Hernando, CA Wagner
    PLoS ONE, 2018;13(5):e0195427.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: WB
  2. Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
    Nat. Med, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: WB
  3. Ligation of TLR7 on CD19(+) CD1d(hi) B cells suppresses allergic lung inflammation via regulatory T cells.
    Authors: Khan A, Amu S, Saunders S, Hams E, Blackshields G, Leonard M, Weaver C, Sparwasser T, Sheils O, Fallon P
    Eur J Immunol, 2015;45(6):1842-54.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Neut
  4. The Processed Amino-Terminal Fragment of Human TLR7 Acts as a Chaperone To Direct Human TLR7 into Endosomes.
    Authors: Hipp M, Shepherd D, Booth S, Waithe D, Reis E Sousa C, Cerundolo V
    J Immunol, 2015;194(11):5417-25.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  5. Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.
    Authors: Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C
    Kidney Int, 2014;85(6):1340-50.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: WB
  6. Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
    Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
    Sci Rep, 2014;4(0):6315.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  7. Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
    Authors: Suryawanshi A, Mulik S, Sharma S
    J. Immunol., 2011;186(6):3653-65.
    Species: N/A
    Sample Types: N/A
    Applications: WB
  8. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
    Authors: Reddy PB, Sehrawat S, Suryawanshi A
    J. Immunol., 2011;187(11):5745-55.
    Species: N/A
    Sample Types: N/A
    Applications: WB

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