Rat IgG HRP-conjugated Antibody

Catalog # Availability Size / Price Qty
HAF005
Detection of Rat IgG Primary Antibody by Western Blot
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Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (5)

Rat IgG HRP-conjugated Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat IgG1, IgG2A, IgG2B heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with human IgG and rabbit IgG is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Rat IgG
Formulation
Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
Label
HRP (Horseradish Peroxidase)(Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Western Blot
Use at a 1:1000 dilution.
 
Simple Western
Use at a 1:50 dilution.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Rat IgG Primary Antibody by Western Blot View Larger

Detection of Rat IgG Primary Antibody by Western Blot Western blot shows detection of Rat Anti-Human/Mouse Wnt-5a Monoclonal Antibody (Catalog # MAB645) using a 1:1000 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Rat IgG Primary Antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Rat IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of Rat Anti-Mouse S100A9 Monoclonal Antibody (Catalog # MAB2065) using a 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

Product Datasheets

Citations for Rat IgG HRP-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Skeletal muscle derived Musclin protects the heart during pathological overload
    Authors: M Szaroszyk, B Kattih, A Martin-Gar, FA Trogisch, GM Dittrich, A Grund, A Abouissa, K Derlin, M Meier, T Holler, M Korf-Kling, K Völker, T Garfias Ma, C Pablo Tort, M Boschmann, N Huang, N Froese, C Zwadlo, M Malek Moha, X Luo, M Wagner, J Cordero, R Geffers, S Batkai, T Thum, N Bork, VO Nikolaev, OJ Müller, HA Katus, A El-Armouch, T Kraft, J Springer, G Dobreva, KC Wollert, J Fielitz, S von Haehli, M Kuhn, J Bauersachs, J Heineke
    Nature Communications, 2022;13(1):149.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. The effects of BAFF and APRIL signaling on non-small cell lung cancer cell proliferation and invasiveness
    Authors: M Warakomska, M Tynecka, D Lemancewic, K Grubczak, J Dzieciol, M Moniuszko, A Eljaszewic, L Bolkun
    Oncology Letters, 2021;22(4):728.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Cyclo(Val-Pro) and Cyclo(Leu-Hydroxy-Pro) from Pseudomonas sp. (ABS-36) alleviates acute and chronic renal injury under in vitro and in vivo models (Ischemic reperfusion and unilateral ureter obstruction)
    Authors: K Hira, P Sharma, A Mahale, O Prakash Ku, A Sajeli Beg
    International immunopharmacology, 2021;103(0):108494.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
    Authors: LG Lima, S Ham, H Shin, EPZ Chai, ESH Lek, RJ Lobb, AF Müller, S Mathivanan, B Yeo, Y Choi, BS Parker, A Möller
    Nature Communications, 2021;12(1):3543.
  5. Overexpression of the receptor for advanced glycation end-products in the auditory cortex of rats with noise-induced hearing loss
    Authors: CH Lee, KW Kim, DH Lee, SM Lee, SY Kim
    Bmc Neuroscience, 2021;22(1):38.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice
    Authors: J Frew, HB Nygaard
    Acta neuropathologica communications, 2021;9(1):57.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Interleukin-18 levels and mouse Leydig cell apoptosis during lipopolysaccharide-induced acute inflammatory conditions
    Authors: T Inoue, M Aoyama-Ish, M Uemura, H Yamashita, Y Koga, M Terashima, M Usami, J Kotani, J Hirata
    J. Reprod. Immunol., 2020;141(0):103167.
    Species: Rat
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  8. Regulation of antitumour CD8 T-cell immunity and checkpoint blockade immunotherapy by Neuropilin-1
    Authors: M Leclerc, E Voilin, G Gros, S Corgnac, V de Montpré, P Validire, G Bismuth, F Mami-Choua
    Nat Commun, 2019;10(1):3345.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  9. Propranolol regulates ERK1/2 signaling pathway and promotes chronic wound healing in diabetic rats
    Authors: X Chang, S Li, XD Xue, F Chang
    Eur Rev Med Pharmacol Sci, 2019;23(10):4498-4506.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Deletion of Galectin-3 attenuates acute pancreatitis in mice by affecting activation of innate inflammatory cells
    Authors: B Stojanovic, I Jovanovic, BS Stojanovic, MD Stojanovic, N Gajovic, G Radosavlje, J Pantic, N Arsenijevi, ML Lukic
    Eur. J. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Cryo-EM structure of the essential ribosome assembly AAA-ATPase Rix7
    Authors: YH Lo, M Sobhany, AL Hsu, BL Ford, JM Krahn, MJ Borgnia, RE Stanley
    Nat Commun, 2019;10(1):513.
    Applications: Western Blot
  12. A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor
    Authors: DT Dao, L Anez-Busti, SS Jabbouri, A Pan, H Kishikawa, PD Mitchell, GL Fell, MA Baker, RS Watnick, H Chen, MS Rogers, DR Bielenberg, M Puder
    PLoS ONE, 2018;13(12):e0208579.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Regulation of vitamin D metabolizing enzymes in murine renal and extrarenal tissues by dietary phosphate, FGF23, and 1,25(OH)2D3
    Authors: L Kägi, C Bettoni, EM Pastor-Arr, U Schnitzbau, N Hernando, CA Wagner
    PLoS ONE, 2018;13(5):e0195427.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
    Nat. Med, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. The Processed Amino-Terminal Fragment of Human TLR7 Acts as a Chaperone To Direct Human TLR7 into Endosomes.
    Authors: Hipp M, Shepherd D, Booth S, Waithe D, Reis E Sousa C, Cerundolo V
    J Immunol, 2015;194(11):5417-25.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Ligation of TLR7 on CD19(+) CD1d(hi) B cells suppresses allergic lung inflammation via regulatory T cells.
    Authors: Khan A, Amu S, Saunders S, Hams E, Blackshields G, Leonard M, Weaver C, Sparwasser T, Sheils O, Fallon P
    Eur J Immunol, 2015;45(6):1842-54.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Neutralization
  17. Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
    Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
    Sci Rep, 2014;4(0):6315.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.
    Authors: Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C
    Kidney Int, 2014;85(6):1340-50.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
    Authors: Suryawanshi A, Mulik S, Sharma S
    J. Immunol., 2011;186(6):3653-65.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot
  20. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
    Authors: Reddy PB, Sehrawat S, Suryawanshi A
    J. Immunol., 2011;187(11):5745-55.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot

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Reviews for Rat IgG HRP-conjugated Antibody

Average Rating: 4.4 (Based on 5 Reviews)

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Rat IgG HRP-conjugated Antibody
By Amruta Narayanappa on 11/27/2020
Application: Western Blot Sample Tested: bEnd.3 mouse endothelial cell Species: Mouse

It works great for western blot


Rat IgG HRP-conjugated Antibody
By Anonymous on 11/20/2019
Application: Western Blot Sample Tested: Mv1Lu mink lung epithelial cell line Species: Mink

Rat IgG HRP-conjugated Antibody
By Anonymous on 06/06/2019
Application: Western Blot Sample Tested: Lung single-cell suspension Species: Mouse

Rat IgG HRP-conjugated Antibody
By Anonymous on 05/20/2019
Application: Western Blot Sample Tested: Blood mononuclear cells (PBMCs) Species: Mouse

Primary antibody: rat anti-mouse CD45.
Sample: 40 ug and 100 ug cell lysis.


Rat IgG HRP-conjugated Antibody
By Anonymous on 09/30/2018
Application: Western Blot Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line Species: Mouse