hh Rat IgG HRP-conjugated Antibody HAF005: R&D Systems

Rat IgG HRP-conjugated Antibody

(7 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat IgG1, IgG2A, IgG2B heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with human IgG and rabbit IgG is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Rat IgG
  • Formulation
    Supplied as a 0.2 um filtered solution in Sodium Phosphate and Proclin 300.
  • Label
    HRP (Horseradish Peroxidase)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    Use at a 1:1000 dilution.
  • Simple Western
    Use at a 1:50 dilution.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat IgGPrimary Antibody by Western Blot Western blot shows detection of Rat Anti-Human/Mouse Wnt-5a MonoclonalAntibody (Catalog # MAB645)using a 1:1000 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody
(Catalog # HAF005).  This experiment wasconducted under reducing conditions and using ImmunoblotBuffer Group 1.
Detection of Rat IgG Primary Antibody by Simple WesternTM. Simple Western lane view shows detection of RatAnti-Mouse S100A9 Monoclonal Antibody
(Catalog #MAB2065)using a 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions andusing the 12-230 kDa separation system.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Do not freeze.
    • 6 months from date of receipt, 2 to 8 °C as supplied.
Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G
  • Alternate Names:
    IgG; immunoglobulin heavy constant gamma 1 (G1m marker)
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Species
Applications
Sample Type
  1. Fasting selectively blocks development of acute lymphoblastic leukemia via leptin-receptor upregulation
    Nat. Med, 2016;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. The Processed Amino-Terminal Fragment of Human TLR7 Acts as a Chaperone To Direct Human TLR7 into Endosomes.
    Authors: Hipp M, Shepherd D, Booth S, Waithe D, Reis E Sousa C, Cerundolo V
    J Immunol, 2015;194(11):5417-25.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Ligation of TLR7 on CD19(+) CD1d(hi) B cells suppresses allergic lung inflammation via regulatory T cells.
    Authors: Khan A, Amu S, Saunders S, Hams E, Blackshields G, Leonard M, Weaver C, Sparwasser T, Sheils O, Fallon P
    Eur J Immunol, 2015;45(6):1842-54.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: Neut
  4. Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.
    Authors: Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C
    Kidney Int, 2014;85(6):1340-50.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
  5. Stage of breast cancer progression influences cellular response to activation of the WNT/planar cell polarity pathway.
    Authors: MacMillan, Connor D, Leong, Hon S, Dales, David W, Robertson, Amy E, Lewis, John D, Chambers, Ann F, Tuck, Alan B
    Sci Rep, 2014;4(0):6315.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Ocular neovascularization caused by herpes simplex virus type 1 infection results from breakdown of binding between vascular endothelial growth factor A and its soluble receptor.
    Authors: Suryawanshi A, Mulik S, Sharma S
    J. Immunol., 2011;186(6):3653-65.
    Species: N/A
    Sample Type: N/A
    Application: WB
  7. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
    Authors: Reddy PB, Sehrawat S, Suryawanshi A
    J. Immunol., 2011;187(11):5745-55.
    Species: N/A
    Sample Type: N/A
    Application: WB
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