Rat LIX Antibody Summary
Accession # P97885.1
Rat LIX Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by LIX and Neutralization by Rat LIX Antibody. Recombinant Rat LIX (Catalog # 543-RL) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat LIX (0.2 µg/mL) is neutralized (green line) by increasing concen-trations of Rat LIX Monoclonal Antibody (Catalog # MAB543). The ND50is typically 1-5 µg/mL.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
LIX (Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-10 kDa member of the Intercrine alpha (or CxC) family of chemokines. It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium. As a chemokine, LIX demonstrates chemotactic properties. It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages. Notably, circulating LIX is not derived from fibroblasts, but platelets. This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX. Rat LIX is synthesized as a 130 amino acid (aa) precursor that contains a 37 aa signal sequence plus a 93 aa mature region (aa 38-130) that likely possesses a cleavable 13 aa C-terminus (SwissProt #:P97885). The mature region possesses a receptor-binding ELR/GluLeuArg motif between aa 47-50, and an alpha -family characteristic CxC motif between aa 50-52. Although there are no known splice variants of rat LIX, based on mouse, considerable proteolytic processing likely occurs at both the N- and C-termini over aa 38-130. This may reduce the MW in SDS-Page by as much as 3 kDa. The majority of LIX could be expected to start between aa 43-46, and this is likely to be positively correlated with bioactivity. Over aa 38-117, rat LIX shares 73% aa sequence identity with mouse LIX. Although not a strict ortholog, rat LIX also shares 64% aa sequence identity with human GCP-2.
Citation for Rat LIX Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
Authors: Miura TA, Wang J, Holmes KV, Mason RJ
Sample Types: Cell Culture Supernates
Applications: ELISA Development
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Rat LIX Antibody
There are currently no reviews for this product. Be the first to review Rat LIX Antibody and earn rewards!
Have you used Rat LIX Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image