Rat LIX Antibody

(1 citations)   
  • Species Reactivity
    Rat
  • Specificity
    Detects rat LIX in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) LIX is observed, 5% cross-reactivity with recombinant rat (rr) CXCL2 is observed, and no cross-reactivity with recombinant human rhCXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, or 13, rmCXCL1, 2, 9, 10, 11, 12, 13, or 15, recombinant porcine (rp) CXCL8, or rrCXCL1 or 3 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 135803
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant rat LIX
    Ala38-Arg125, Ala38-Lys117
    Accession # P97885.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Rat LIX (Catalog # 543-RL) under non-reducing conditions only
    • Rat LIX Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Rat LIX Antibody (Catalog # MAB543)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Rat LIX Biotinylated Antibody (Catalog # BAF543)
  • ELISA Standard
     
    Recombinant Rat LIX Protein (Catalog # 543-RL)
  • Neutralization
    Measured by its ability to neutralize LIX-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 0.2 µg/mL Recombinant Rat LIX.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by LIX and Neutralization by Rat LIX Antibody. Recombinant Rat LIX (Catalog # 543-RL) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat LIX (0.2 µg/mL) is neutralized (green line) by increasing concen­trations of Rat LIX Monoclonal Antibody (Catalog # MAB543). The ND50 is typically 1-5 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIX

LIX (Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-10 kDa member of the Intercrine alpha (or CxC) family of chemokines.  It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium.  As a chemokine, LIX demonstrates chemotactic properties.  It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages.  Notably, circulating LIX is not derived from fibroblasts, but platelets.  This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX.  Rat LIX is synthesized as a 130 amino acid (aa) precursor that contains a 37 aa signal sequence plus a 93 aa mature region (aa 38-130) that likely possesses a cleavable 13 aa C-terminus (SwissProt #:P97885).  The mature region possesses a receptor-binding ELR/GluLeuArg motif between aa 47-50, and an alpha -family characteristic CxC motif between aa 50-52.  Although there are no known splice variants of rat LIX, based on mouse, considerable proteolytic processing likely occurs at both the N- and C-termini over aa 38-130.  This may reduce the MW in SDS-Page by as much as 3 kDa.  The majority of LIX could be expected to start between aa 43-46, and this is likely to be positively correlated with bioactivity.  Over aa 38-117, rat LIX shares 73% aa sequence identity with mouse LIX.  Although not a strict ortholog, rat LIX also shares 64% aa sequence identity with human GCP-2.

 

  • Entrez Gene IDs:
    20311 (Mouse); 60665 (Rat)
  • Alternate Names:
    LIX
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
    Authors: Miura TA, Wang J, Holmes KV, Mason RJ
    Virology, 2007;369(2):288-98.
    Species: Rat
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
Isotype Controls
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Mouse IgG1 Isotype Control

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Mouse IgG1 Isotype Control

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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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