Rat LIX Antibody

Catalog # Availability Size / Price Qty
MAB543
MAB543-SP
Rat LIX Antibody in Data
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Product Details
Citations (1)
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Rat LIX Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat LIX in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) LIX is observed, 5% cross-reactivity with recombinant rat (rr) CXCL2 is observed, and no cross-reactivity with recombinant human rhCXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, or 13, rmCXCL1, 2, 9, 10, 11, 12, 13, or 15, recombinant porcine (rp) CXCL8, or rrCXCL1 or 3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 135803
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat LIX
Ala38-Arg125, Ala38-Lys117
Accession # P97885.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat LIX (Catalog # 543-RL) under non-reducing conditions only

Rat LIX Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Rat LIX Biotinylated Antibody (Catalog # BAF543)

Standard: Recombinant Rat LIX Protein (Catalog # 543-RL)

Neutralization
Measured by its ability to neutralize LIX-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 0.2 µg/mL Recombinant Rat LIX.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Chemotaxis Induced by LIX and Neutralization by Rat LIX Antibody. View Larger

Chemotaxis Induced by LIX and Neutralization by Rat LIX Antibody. Recombinant Rat LIX (Catalog # 543-RL) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat LIX (0.2 µg/mL) is neutralized (green line) by increasing concen-trations of Rat LIX Monoclonal Antibody (Catalog # MAB543). The ND50is typically 1-5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIX

LIX (Liposaccharide-Induced CXC chemokine; also GARG-8) is a secreted 8-10 kDa member of the Intercrine alpha (or CxC) family of chemokines.  It is widely expressed, being produced by diverse cell types such as fibroblasts, thymic epithelium, platelets, vascular endothelium, hepatocytes, lung type II alveolar cells and ileal columnar epithelium.  As a chemokine, LIX demonstrates chemotactic properties.  It induces the chemotaxis of neutrophils and endothelial cells, and also promotes TNF-alpha secretion from mast cells and macrophages.  Notably, circulating LIX is not derived from fibroblasts, but platelets.  This suggests that neutrophil homeostasis/chemotaxis is a function of local resident cell activation and LIX secretion, not generally circulating LIX.  Rat LIX is synthesized as a 130 amino acid (aa) precursor that contains a 37 aa signal sequence plus a 93 aa mature region (aa 38-130) that likely possesses a cleavable 13 aa C-terminus (SwissProt #:P97885).  The mature region possesses a receptor-binding ELR/GluLeuArg motif between aa 47-50, and an alpha -family characteristic CxC motif between aa 50-52.  Although there are no known splice variants of rat LIX, based on mouse, considerable proteolytic processing likely occurs at both the N- and C-termini over aa 38-130.  This may reduce the MW in SDS-Page by as much as 3 kDa.  The majority of LIX could be expected to start between aa 43-46, and this is likely to be positively correlated with bioactivity.  Over aa 38-117, rat LIX shares 73% aa sequence identity with mouse LIX.  Although not a strict ortholog, rat LIX also shares 64% aa sequence identity with human GCP-2.

 

Entrez Gene IDs
20311 (Mouse); 60665 (Rat)
Alternate Names
LIX

Product Datasheets

Citation for Rat LIX Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Rat coronaviruses infect rat alveolar type I epithelial cells and induce expression of CXC chemokines.
    Authors: Miura TA, Wang J, Holmes KV, Mason RJ
    Virology, 2007;369(2):288-98.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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