Rat PBEF/Visfatin Antibody Summary
Accession # Q80Z29
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Rat PBEF by Western Blot. Western blot shows lysates of H4‑II‑E‑C3 rat hepatoma cell line and rat liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Rat PBEF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6384) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PBEF at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
PBEF (pre-B cell colony-enhancing factor; also visfatin and nicotinamide phosphoribosyltransferase/Nampt) is a 52‑54 kDa member of the NAPRTase family of molecules. It is a dimer that is found both intracellularly and extracellularly, where it participates in NAD synthesis and induces proinflammatory cytokine synthesis, respectively. PBEF has also been reported to show insulin-like effects on adipocytes by binding to the insulin receptor. Cells known to express PBEF include visceral adipocytes, neutrophils, and capillary endothelium. Rat PBEF is 491 amino acids in length. It contains no signal sequence but is secreted. Potential phosphorylation sites exist at Tyr34, Tyr188 and Ser398. Full‑length (aa 1‑491) rat PBEF shares 99% and 95% aa identity with mouse and human PBEF, respectively.
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