Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-1 is a glycoprotein with a molecular mass of 32‑34 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. TIMP-1 also associates C-terminally with pro-MMP-9 in a complex which may play a role in regulating activation. Independent of MMPs, TIMP-1 has been shown to have a role in tissue homeostasis.
Key Product Details
Species Reactivity
Rat
Applications
Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 150906
Loading...
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat TIMP-1
Cys24-Ala217
Accession # P30120
Cys24-Ala217
Accession # P30120
Specificity
Detects rat TIMP-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) TIMP-1, recombinant mouse TIMP-1, rhTIMP-2, or rhTIMP-4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Rat TIMP‑1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Rat TIMP‑1 (Catalog # 580-RT), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Rat TIMP‑1 (Catalog # 580-RT), see our available Western blot detection antibodies
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: TIMP-1
Long Name
Tissue Inhibitors of Metalloproteinases 1
Alternate Names
TIMP1
Gene Symbol
TIMP1
UniProt
Additional TIMP-1 Products
Product Documents for Rat TIMP‑1 Antibody
Product Specific Notices for Rat TIMP‑1 Antibody
For research use only
Related Research Areas
Customer Reviews for Rat TIMP‑1 Antibody
There are currently no reviews for this product. Be the first to review Rat TIMP‑1 Antibody and earn rewards!
Have you used Rat TIMP‑1 Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review