Recombinant Human BMP-4 Animal-Free Protein

Catalog # Availability Size / Price Qty
AFL314E-010
AFL314E-050
AFL314E-01M
AFL314E-250
Recombinant Human BMP-4 Animal-Free Protein Data
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Recombinant Human BMP-4 Animal-Free Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 3-21 ng/mL.
Source
E. coli-derived human BMP-4 protein
Lys303-Arg408
Produced using non-animal reagents in an animal-free laboratory.
Accession #
N-terminal Sequence
Analysis
Lys303
Structure / Form
Disulfide linked homodimer
Predicted Molecular Mass
12 kDa
SDS-PAGE
8-9 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AFL314E

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100-200 μg/mL in 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 3-21 ng/mL.

SDS-PAGE View Larger

2 μg/lane of Animal-FreeTM Recombinant Human BMP-4 Protein (AFL314E) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 8-9 kDa and 20-22 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BMP-4

Bone morphogenetic protein 4 (BMP-4) is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation, epidermal determination, suppression of neural induction, the development of multiple organs, and tissue repair (1-5). It is an integral part of many stem cell differentiation pathways, including lung tissue, (6), adipogenesis (7) and osteogenesis (8, 9). The human BMP-4 precursor contains a 273 amino acid (aa) propeptide and a 116 amino acid (aa) mature protein (10). Processing of the propeptide by furin or proprotein convertase 6 enables the formation of the mature disulfide-linked homodimeric BMP-4 and facilitates its secretion. Similar intracellular processes may lead to the formation and recreation of BMP4/BMP7 disulfide-linked heterodimer (11-13). Mature human and mouse BMP-4 share 98% aa sequence identity. Human BMP-4 shares 85% aa sequence identity with human BMP-2 and less than 50% with other human BMPs. In Xenopus, BMP-4 dimers provide ventralizing signals for existing mesoderm (14). BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors (15, 16). The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans (17-19).

References
  1. Zhang, P. et al. (2008) Blood 111:1933.
  2. Gambaro, K. et al. (2006) Cell Death Differ. 13:1075.
  3. Simic, P. and S. Vukicevic (2005) Cytokine Growth Factor Rev. 16:299.
  4. Sadlon, T.J. et al. (2004) Stem Cells 22:457.
  5. Frank, D.B. et al. (2005) Circ. Res. 97:496.
  6. Lee, J-H. et al. (2014) Cell. 156:440.
  7. Tang, QQ. et Lane MD. (2012) Annu Rev. Biochem. 81:715.
  8. Urist, MR. (1965). Science. 150:893.
  9. Bandyopadhyay, A. et al. (2006) Plos Genetics. 2:e216.
  10. Wozney, J. et al. (1988) Science 242:1528.
  11. Cui, Y. et al. (1998) EMBO J. 17:4735.
  12. Cui, Y. et al. (2001) Genes Dev. 15:2797.
  13. Aono, A. et al. (1995) Biochem. Biophys. Res. Commun. 210:670.
  14. Nishimatsu, S. and G.H. Thomsen (1998) Mech. Dev. 74:75.
  15. Chen, D. et al. (2004) Growth Factors 22:233.
  16. Lavery, K. et al. (2008) J. Biol. Chem. April 24 epub.
  17. Rosen, V. (2006) Ann. N.Y. Acad. Sci. 1068:19.
  18. Jones, C.M. and J.C. Smith (1998) Dev. Biol. 194:12.
  19. Takada, T. et al. (2003) J. Biol. Chem. 278:43229.
Long Name
Bone Morphogenetic Protein 4
Entrez Gene IDs
652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
Alternate Names
BMP2B; BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME

Manufacturing Specifications

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.  

 Production

  • All molecular biology procedures use animal-free media and dedicated labware.
  • Dedicated fermentors are utilized in committed animal-free areas. 

Purification 

  • Protein purification columns are animal-free.
  • Bulk proteins are filtered using animal-free filters.
  • Purified proteins are stored in animal-free containers in a dedicated cold storage room.

    Quality Assurance 

    • Low Endotoxin Level.
    • No impairment of biological activity.
    • High quality product obtained under stringent conditions.
    • For ex vivo research or bioproduction, additional documentation can be provided. 

    Please read our complete Animal-Free Statement

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