Recombinant Human CD38 His-tag Avi-tag Protein, CF

R&D Systems | Catalog # AVI2404

Biotinylated
R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems HEK293-derived Recombinant Human CD38 His-tag Avi-tag Protein (AVI2404)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Binding Activity, Enzyme Activity
View all CD38 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD38 protein
Human CD38
(Val43-Ile300)
Accession # P28907.2		 HHHHHH Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val43

Predicted Molecular Mass

33 kDa

SDS-PAGE

40-52 kDa, under reducing conditions

Activity

Measured by its ability to convert the substrate nicotinamide guanine dinucleotide (NGD+) to cyclic GDP-ribose. Graeff, R.M. et al. (1994) J. Biol. Chem. 269:30260.
The specific activity is >1500 pmol/min/μg, as measured under the described conditions.

Measured by its binding ability in a functional ELISA.
When Human CD38 Antibody  (Catalog # AF2404) is immobilized at 1 µg/mL (100 µL/well), it binds to Biotinylated Recombinant Human CD38 His-tag Avi-tag (Catalog # AVI2404) with an ED50 of 1-6 ng/mL.

Scientific Data Images for Recombinant Human CD38 His-tag Avi-tag Protein, CF

Recombinant Human CD38 His-tag Avi-tag Protein Binding Activity

Recombinant Human CD38 His-tag Avi-tag Protein Binding Activity

When Human CD38 Antibody (AF2404) is immobilized at 1 μg/mL (100 μL/well), it binds to Biotinylated Recombinant Human CD38 His-tag Avi-tag (AVI2404) with an ED50 of 1-6 ng/mL.
Recombinant Human CD38 His-tag Avi-tag Protein SDS-PAGE

Recombinant Human CD38 His-tag Avi-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human CD38 His-tag Avi-tag Protein (AVI2404) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-52 kDa.

Formulation, Preparation, and Storage

AVI2404
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: CD38

CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta cells (4, 5). Our Avi-tag Biotinylated Recombinant Human CD38 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
  2. Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
  3. Sun, L. et al. (1999) J. Cell Biol. 146:1161.
  4. Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
  5. Kato, I. et al. (1995) J. Biol. Chem. 270:30045.

Long Name

Cluster of Differentiation 38

Alternate Names

ADP-ribosyl Cyclase, CD38, Cyclic ADP-ribose Hydrolase

Entrez Gene IDs

952 (Human); 12494 (Mouse); 25668 (Rat); 102126394 (Cynomolgus Monkey)

Gene Symbol

CD38

Additional CD38 Products

Product Documents for Recombinant Human CD38 His-tag Avi-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CD38 His-tag Avi-tag Protein, CF

For research use only

Customer Reviews for Recombinant Human CD38 His-tag Avi-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human CD38 His-tag Avi-tag Protein, CF and earn rewards!

Have you used Recombinant Human CD38 His-tag Avi-tag Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Recombinant Human CD38 His-tag Avi-tag Protein, CF (AVI2404):

Materials
  •  Assay Buffer: 50 mM MES, pH 6.5
  •  Recombinant Human CD38 His-tag Avi-tag (rhCD38/His/Avi-tag) (Catalog # AVI2404)
  •  Substrate: Nicotinamide guanine dinucleotide (NGD+) (Sigma, Catalog # N5131), 10 mM stock in deionized water
  •  F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
  •  Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
  1. Dilute rhCD38/His/Avi-tag to 2 ng/µL in Assay Buffer.
  2. Dilute Substrate to 200 µM in Assay Buffer.
  3. Load in plate, 50 µL of 2 ng/µL rhCD38/His/Avi-tag, and start the reaction by adding 50 µL of 200 µM Substrate.  Include a Substrate Blank containing 50 µL of Assay Buffer and 50 µL of 200 µM Substrate.
  4. Read at excitation and emission wavelengths of 300 nm and 410 nm (top read), respectively in kinetic mode for 5 minutes.
  5. Calculate specific activity:
 

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)
amount of enzyme (µg)

*Adjusted for Substrate Blank
**Derived using calibration standard cyclic GDP ribose (cGDPR).

Per Well:
  • rhCD38/His/Avi-tag: 0.1 µg
  • Substrate: 100 µM

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes