Detection of Human CD38 by Western Blot. Western blot shows lysates of MOLT‑4 human acute lymphoblastic leukemia cell line, RPMI 8226 human multiple myeloma cell line, and CEM human T-lymphoblastoid cell line. PVDF membrane was probed with 0.1 µg/mL of Sheep Anti-Human CD38 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2404) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific and diffuse band was detected for CD38 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD38, also known as ADP-ribosyl cyclase, is a Type II integral membrane protein. The enzyme is able to transform NAD(P)+ into three different products with calcium mobilizing ability, cyclic ADP-ribose, NAADP+, and ADP-ribose (1). CD38 is expressed in B and T lymphocytes, osteoclasts, and in cardiac, pancreatic, liver and kidney cells (2, 3). Through its production of cyclic ADP-ribose, CD38 modulates calcium-mediated signal transduction in many types of cells, including neutrophils and pancreatic beta cells (4, 5).
Schuber, F. and F.E. Lund (2004) Curr. Mol. Med. 4:249.
Jackson, D.G. and J.I. Bell (1990) J. Immunol. 144:2811.
Sun, L. et al. (1999) J. Cell Biol. 146:1161.
Partida-Sanchez, S. et al. (2001) Nature Med. 7:1209.
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