Recombinant Human gp130 Fc Chimera Avi-tag Protein, CF

Biotinylated
Catalog # Availability Size / Price Qty
AVI10929-025
Biotinylated Recombinant Human gp130 Fc Chimera Avi-tag Protein SDS-PAGE.
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Recombinant Human gp130 Fc Chimera Avi-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human gp130 Fc Chimera Avi-tag is captured on a Streptavidin Coated Plate (Catalog # CP004), it binds to Recombinant Human IL-6 (Catalog # 7270-IL) in the presence of Recombinant Human IL-6R alpha (Catalog # 227-SR). The ED50 for this binding is 1-10 ng/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human gp130 protein
Human gp130
(Glu23-Ile618)
Accession # AAA59155.1
IEGRMDHuman IgG1
(Pro100-Lys330)
Avi-tag
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Glu23
Structure / Form
Biotinylated via Avi-tag
Predicted Molecular Mass
96 kDa
SDS-PAGE
115-127 kDa, under reducing conditions.

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI10929

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE Biotinylated Recombinant Human gp130 Fc Chimera Avi-tag Protein SDS-PAGE. View Larger

2 μg/lane of Biotinylated Recombinant Human gp130 Fc Chimera Avi-tag Protein (Catalog # AVI10929) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 115-127 kDa and 230-250 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

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Background: gp130

gp130 (glycoprotein 130, also known as IL-6RB and IL-6ST) is a subunit of receptor complexes for several cytokines that mediate highly diverse biological processes. These include functionally and structurally related cytokines within the IL-6 and IL-12 family including IL-6, IL-11, IL-27, IL-35, IL-39, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1), novel neutrophin-1/B cell stimulating factor-3 or cardiotrophin-like cytokine (CLC) and neuropoetin (NP) (1, 2). gp130 is ubiquitously expressed in all cell types. Depending on the cytokine, binding of ligand leads to the formation of signalling complexes that trigger a sophisticated signalling machinery downstream of gp130. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R (3-6). Mature human CP130 consists of a 597 amino acid (aa) extracellular region (ECD), a 22 aa transmembrane domain, and a 277 aa cytoplasmic region. Within the ECD, human gp130 shares 73% and 75% aa sequence identity with mouse and rat gp130, respectively. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. At the present time, the in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities. Our Avi-tag Biotinylated Recombinant Human gp130 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Xu, S. and N. Neamati (2013) Expert Opin. Ther. Targets 17:1303.
  2. Ritter, K. et al. (2020) Cells 9:2695.
  3. Martinez-Perez, C, et al. (2021) J. Pers. Med. 11:618.
  4. Silver, J.S. and C.A. Hunter (2010) J. Leukoc. Biol. 88:1145.
  5. Narazaki, M. et al. (1993) Blood 82:1120.
  6. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Long Name
Glycoprotein 130
Entrez Gene IDs
3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names
CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

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