Recombinant Human IL-6 (HEK293-expressed) Protein

Carrier Free

Catalog # Availability Size / Price Qty
7270-IL-025/CF
7270-IL-010/CF
7270-IL-100/CF

With Carrier

Catalog # Availability Size / Price Qty
7270-IL-025
Recombinant Human IL-6 (HEK293-expressed) Protein Bioactivity
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Product Details
Citations (5)
FAQs
Reviews (2)

Recombinant Human IL-6 (HEK293-expressed) Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is 0.1-0.6 ng/mL.
Source
Human embryonic kidney cell, HEK293-derived human IL-6 protein
Val30-Met212
Accession #
N-terminal Sequence
Analysis
Ala28 & Val30
Predicted Molecular Mass
20.8 kDa
SDS-PAGE
22 kDa, reducing conditions

Product Datasheets

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7270-IL (with carrier)

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7270-IL/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7270-IL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7270-IL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human IL-6 (HEK293-expressed) Protein Bioactivity View Larger

Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. The ED50 for this effect is 0.1-0.6 ng/mL.

Reconstitution Calculator

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Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6 (6). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (7-10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 13). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 14).

References
  1. Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249.
  2. Schuett, H. et al. (2009) Thromb. Haemost. 102:215.
  3. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254.
  4. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
  5. Mihara, M. et al. (2012) Clin. Sci. (Lond.) 122:143.
  6. Hirano, T. et al. (1986) Nature 324:73.
  7. Kestler, D.P. et al. (1995) Blood 86:4559.
  8. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169.
  9. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48.
  10. Alberti, L. et al. (2005) Cancer Res. 65:2.
  11. Murakami, M. et al. (1993) Science 260:1808.
  12. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  13. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
  14. Cerutti, A. et al. (1998) J. Immunol. 160:2145.
Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Citations for Recombinant Human IL-6 (HEK293-expressed) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Deletion of STAT3 from Foxd1 cell population protects mice from kidney fibrosis by inhibiting pericytes trans-differentiation and migration
    Authors: AK Ajay, L Zhao, S Vig, M Fujiwara, S Thakurela, S Jadhav, A Cho, IJ Chiu, Y Ding, K Ramachandr, A Mithal, A Bhatt, P Chaluvadi, MK Gupta, SI Shah, VS Sabbisetti, AM Waaga-Gass, DA Frank, G Murugaiyan, JV Bonventre, LL Hsiao
    Cell Reports, 2022;38(10):110473.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. GLI1 activates pro-fibrotic pathways in myelofibrosis fibrocytes
    Authors: T Manshouri, I Veletic, P Li, CC Yin, SM Post, S Verstovsek, Z Estrov
    Cell Death & Disease, 2022;13(5):481.
    Species: Xenografts
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Kynurenine metabolites predict survival in pulmonary arterial hypertension: A role for IL-6/IL-6Ralpha
    Authors: Z Cai, S Tian, T Klein, L Tu, LW Geenen, T Koudstaal, AE van den Bo, YB de Rijke, IKM Reiss, E Boersma, C van der Le, M Van Faasse, I Kema, DJ Duncker, KA Boomars, K Tran-Lundm, C Guignabert, D Merkus
    Scientific Reports, 2022;12(1):12326.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasma
    Authors: S Jadhav, S Tripathi, A Chandrekar, SS Waikar, LL Hsiao
    PLoS ONE, 2021;16(1):e0245614.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Capture
  5. The inhibitory effect of TU-100 on hepatic stellate cell activation in the tumor microenvironment
    Authors: Y Wada, K Tokuda, Y Morine, S Okikawa, S Yamashita, T Ikemoto, S Imura, Y Saito, S Yamada, M Shimada
    Oncotarget, 2020;11(49):4593-4604.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Culture

FAQs

  1. What are the differences between Recombinant Human IL-6 Protein (Catalog # 206-IL) and Recombinant Human IL-6 (HEK293-expressed) Protein (Catalog # 7270-IL)?

    • Recombinant Human IL-6 Protein (Catalog # 206-IL) is raised in E. coli. The Recombinant Human IL-6 (HEK293-expressed) Protein (Catalog # 7270-IL) is raised in HEK293. Because #7270-IL is raised in mammalian cells, it is expected to have post translational modifications that are absent in the E. coli-derived version (206-IL). The HEK293-derived #7270-IL exhibits greater activity than the E. coli-derived #206-IL in the same activity assay.

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Reviews for Recombinant Human IL-6 (HEK293-expressed) Protein

Average Rating: 4.5 (Based on 2 Reviews)

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Recombinant Human IL-6 (HEK293-expressed) Protein
By Anonymous on 04/15/2021
Application: In vitro bioactivity in cell culture
Reason for Rating: Activation of HEK Blue Cells with EC50 of 0.2ng/ml as stated in datasheet.

HEK Blue IL-6 assay from Invivogen


By Anonymous on 07/27/2018
Application: Binding assay/Protein-protein interaction