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Recombinant Human IL-6 GMP Protein, CF

Name: Recombinant Human IL-6 GMP Protein, CF
Brand: R&D Systems
SKU: 206-GMP
Price: 1177 USD
Availability: InStock

R&D Systems | Catalog # 206-GMP

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Citations (4)

Product Documents

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Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Manufacturing Specifications
Specific Notices
Research Areas

Key Product Details

A DMF is on file for this protein

Source

E. coli

Accession Number

P05231.1

Applications

Bioactivity

View all IL-6 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-6 protein
Pro29-Met212
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Pro₂₉-Val-Pro-Pro-Gly-Glu-Asp-Ser-Lys-Asp

Predicted Molecular Mass

20.9 kDa

SDS-PAGE

20-21 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813.
The ED₅₀ for this effect is 0.2‑0.8 ng/mL.
The specific activity of Recombinant Human IL-6 is >1.0 x 10⁸ IU/mg, which is calibrated against the human IL‑6 WHO International Standard (NIBSC code: 21/308).

Host Cell Protein

<0.5 ng per µg of protein when tested by ELISA.

Mycoplasma

Negative for Mycoplasma.

Host Cell DNA

<0.0015 ng of DNA per µg of protein when tested by PCR.

Scientific Data Images for Recombinant Human IL-6 GMP Protein, CF

Recombinant Human IL-6 GMP Protein Bioactivity

GMP-grade Recombinant Human IL‑6 (Catalog # 206‑GMP) stimulates proliferation of T1165.85.2.1 mouse plasmacytoma cell line. The ED₅₀ for this effect is 0.2‑0.8 ng/mL.

Equivalent Bioactivity of GMP and Animal-Free grades of Recombinant Human IL-6

Equivalent bioactivity of GMP (Catalog # 206-GMP) and Animal-Free (AFL206) grades of Recombinant Human IL-6 as measured in cell proliferation assay (orange and green respectively).

Recombinant Human IL-6 GMP Protein SDS-PAGE

1 μg/lane of GMP-grade Recombinant Human IL-6 (Catalog # 206-GMP) was resolved with SDS-PAGE under reducing conditions (R) and visualized by silver staining, showing a single band at 21 kDa.

Recombinant Human IL-6 GMP Protein Mass Spectrometry

MALDI-TOF analysis of GMP-grade Recombinant Human IL-6 (Catalog # 206‑GMP). The major peak corresponds to the calculated molecular mass, 20910 Da. The minor peak at 21127 Da is a matrix-associated artifact of the MALDI-TOF.

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Formulation, Preparation, and Storage

206-GMP

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution	Reconstitute at 100-200 μg/mL in sterile PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression (1-5). Mature human IL-6 is 183 amino acids (aa) in length and shares 39% aa sequence identity with mouse and rat IL-6 (6). Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (7-10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (5). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (5). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 5). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (5, 13). IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1, 2, 5). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 14).

References

Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249.
Schuett, H. et al. (2009) Thromb. Haemost. 102:215.
Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254.
Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
Mihara, M. et al. (2012) Clin. Sci. (Lond.) 122:143.
Hirano, T. et al. (1986) Nature 324:73.
Kestler, D.P. et al. (1995) Blood 86:4559.
Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169.
Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48.
Alberti, L. et al. (2005) Cancer Res. 65:2.
Murakami, M. et al. (1993) Science 260:1808.
Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125.
Cerutti, A. et al. (1998) J. Immunol. 160:2145.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)

Gene Symbol

IL6

UniProt

P05231.1

Additional IL-6 Products

Product Documents for Recombinant Human IL-6 GMP Protein, CF

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Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Manufacturing Specifications

GMP Proteins
R&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.

R&D Systems' quality focus includes:

Designed, manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
Documented and controlled manufacturing process
Control of documentation and process changes by QA
Personnel training programs
Raw material inspection and vendor qualification/monitoring program
Validated equipment, processes and test methods
Equipment calibration and maintenance schedules using a Regulatory Asset Manager
Facility/Utilities maintenance, contamination controls, safety and pest control programs
Material review process for variances
Robust product stability program following relevant ICH guidelines

R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their application. Each product is provided with a lot-specific Certificate of Analysis that contains the product's specifications and test results. Quality control testing may include, but is not limited to:

N-terminal amino acid analysis
SDS-PAGE purity analysis
Molecular weight analysis via mass spectrometry
Endotoxin assessment per USP <85> and Ph. Eur. 2.6.14 guidelines
Bioassay analysis
Microbial testing per USP <71> and Ph. Eur. 2.6.1 guidelines
Host cell protein assessment
Host cell DNA assessment
Mycoplasma assessment

Additional testing and documentation requested by the customer can be arranged at an additional cost.

Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis and St. Paul, Minnesota USA.

R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production