Recombinant Human Integrin alpha 4 beta 7 Protein, CF
Recombinant Human Integrin alpha 4 beta 7 Protein, CF Summary
50-80% will adhere after 60 minutes at 37 °C.
|Human Integrin alpha 4
(Tyr34-Gln970 (R591L & R878Q))
Accession # P13612
|Human Integrin beta 7
Accession # P26010
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and MgCl2.|
|Reconstitution||Reconstitute at 200 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Integrin alpha 4 beta 7/LPAM-1
Integrin alpha 4 beta 7 is an integrin family adhesion receptor that shares subunits with alpha 4 beta 1 (VLA4) and the E-Cadherin receptor, alpha E beta 7 (1). It is a non-covalent heterodimer composed of two type I transmembrane glycoprotein subunits, a 150 kDa alpha 4 (CD49d) subunit and a 130 kDa beta 7 subunit (2, 3). The alpha 4 extracellular domain (ECD) contains an N-terminal beta -propeller structure followed by thigh, calf-1, and calf-2 domains (1). The beta 7 ECD contains a vWFA domain, which interacts with the
alpha 4 beta -propeller to form a binding domain. Metal ion binding sites termed MIDAS and LIMBS promote firm adhesion, and another site termed ADMIDAS is a negative regulatory site that promotes rolling (4-6). The human alpha 4 ECD shares 85% amino acid sequence identity with the mouse, rat, and canine alpha 4 ECD. The human beta 7 ECD shares 88% and 87% amino acid sequence identity with the rat and mouse beta 7 ECD, respectively. Integrin alpha 4 beta 7 binds the mucosal addressin MAdCAM-1, as well as VCAM-1 and Fibronectin (7). Integrin alpha 4 beta 7, which is critical for homing to intestinal mucosa, is induced during T cell activation in Peyer’s patches or mesenteric lymph nodes (8, 9). Its expression requires signals from local dendritic and stromal cells, including secreted retinoic acid (10, 11). The HIV-1 envelope protein gp120 binds to the active form of Integrin alpha 4 beta 7, and this may or may not account for the concentration of HIV-1 virus in the gut-associated lymphoid tissue (GALT) (12-14). Integrin alpha 4 beta 7 may also be involved in lymphocyte trafficking in acute intestinal graft vs. host disease (GVHD) (15).
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- Wagner, N. et al. (1996) Nature 382:366.
- Johansson-Lindbom, B. and W.W. Agace (2007) Immunol. Rev. 215:226.
- Hammerschmidt, S.I. et al. (2008) J. Exp. Med. 205:2483.
- Edele, F. et al. (2008) J. Immunol. 181:3745.
- Arthos, J. et al. (2008) Nat. Immunol. 9:301.
- Cicala, C. et al. (2009) Proc. Natl. Acad. Sci. USA 106:20877.
- Monteiro, P. et al. (2011) J. Immunol. 186:4618.
- Chen, Y.B. et al. (2013) Bone Marrow Transplant. 48:598.
Citations for Recombinant Human Integrin alpha 4 beta 7 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells
Authors: D Karpova, MP Rettig, J Ritchey, D Cancilla, S Christ, L Gehrs, E Chendamara, MO Evbuomwan, M Holt, J Zhang, G Abou-Ezzi, H Celik, E Wiercinska, W Yang, F Gao, LG Eissenberg, RF Heier, SD Arnett, MJ Meyers, MJ Prinsen, DW Griggs, A Trumpp, PG Ruminski, DM Morrow, HB Bonig, DC Link, JF DiPersio
J. Clin. Invest., 2019;129(7):2745-2759.
Sample Types: Protein
Select gp120 V2 domain specific antibodies derived from HIV and SIV infection and vaccination inhibit gp120 binding to ?4?7
Authors: S Lertjuthap, C Cicala, D Van Ryk, M Liu, J Yolitz, D Wei, F Nawaz, A Doyle, B Horowitch, C Park, S Lu, Y Lou, S Wang, R Pan, X Jiang, F Villinger, SN Byrareddy, PJ Santangelo, L Morris, CK Wibmer, K Biris, RD Mason, J Gorman, J Hiatt, E Martinelli, M Roederer, D Fujikawa, G Gorini, G Franchini, A Arakelyan, AA Ansari, K Pattanapan, XP Kong, AS Fauci, J Arthos
PLoS Pathog., 2018;14(8):e1007278.
Sample Types: Magnetic Nanoparticles
Cadherin 26 is an alpha integrin-binding epithelial receptor regulated during allergic inflammation
Authors: JM Caldwell, MH Collins, KA Kemme, JD Sherrill, T Wen, M Rochman, EM Stucke, L Amin, H Tai, PE Putnam, MJ Jim‚nez-Da, MR Wormald, A Porollo, JP Abonia, ME Rothenberg
Mucosal Immunol, 2017;0(0):.
Sample Types: Recombinant Protein
Retinoic acid imprints a mucosal-like phenotype on dendritic cells with an increased ability to fuel HIV-1 infection.
Authors: Guerra-Perez N, Frank I, Veglia F, Aravantinou M, Goode D, Blanchard J, Gettie A, Robbiani M, Martinelli E
J Immunol, 2015;194(5):2415-23.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
What is the amino acid sequence of the acidic and basic tails?
Acidic and basic tails are added to the protein to help facilitate optimal activity. While we generally include sequence information on the product datasheet, the sequences of these tails are considered confidential information.
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