Recombinant Human TIGIT (A103) Fc Chimera Protein, CF

R&D Systems | Catalog # 7898-TGB

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems CHO-derived Recombinant Human TIGIT (A103) Fc Chimera Protein (7898-TGB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

AAI01289

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all TIGIT Proteins and Enzymes »

Why choose R&D Systems TIGIT Protein?

  • Guaranteed Bioactivity and High Purity: Bioactivity tested by functional ELISA and purity determined by SDS-PAGE to be greater than 90%.
  • Lot-to-Lot Consistency: Stringent QC testing performed on each lot to ensure consistent activity and purity.
  • Bulk Quantities Available: Bulk up and save with large mass quantities to meet your research needs. Supply agreements available, partner with us. Please contact us.
  • Most Respected, Most Cited Brand in Proteins: With over 35 years of providing the best recombinant proteins to the scientific community, R&D Systems continues to lead the industry in quality, activity, and purity.

The non-Fc, His-tagged TIGIT protein is available here His-tagged TIGIT Protein

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human TIGIT protein
Human TIGIT
(Met22-Pro141, A103)
Accession # AAI01289		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met22

Predicted Molecular Mass

40 kDa

SDS-PAGE

40-53 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD155/PVR (Catalog # 2530-CD) is coated at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Human TIGIT (A103) Fc Chimera that produces 50% optimal binding response is 40-200 ng/mL.

Reviewed Applications

Read 2 reviews rated 5 using 7898-TGB in the following applications:

Scientific Data Images for Recombinant Human TIGIT (A103) Fc Chimera Protein, CF

Recombinant Human TIGIT (A103) Fc Chimera Protein SEC-MALS.

Recombinant Human TIGIT (A103) Fc Chimera (Catalog # 7898-TGB) has a molecular weight (MW) of 81.6 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Recombinant Human TIGIT Fc Chimera Protein Binding Activity

Recombinant Human TIGIT (A103) Fc Chimera Protein Binding Activity

When Recombinant Human CD155/PVR (2530-CD) is immobilized at 2.5 µg/mL, Recombinant Human TIGIT (A103) Fc Chimera (Catalog # 7898-TGB) binds with an ED50 of 40-200 ng/mL.
Surface plasmon resonance sensorgram of human CD155/PVR protein binding to human TIGIT

Binding of Human TIGIT to CD155/PVR by surface plasmon resonance (SPR).

Recombinant Human CD155/PVR Fc protein (9174-CD) was immobilized on a Biacore Sensor Chip CM5, and binding to Recombinant Human TIGIT (A103) Fc protein (Catalog # 7898-TGB) was measured at a concentration range between 0.531 nM and 544 nM. The double-referenced sensorgram was fit to a 1:1 binding model to determine the binding kinetics and affinity, with an affinity constant of KD=67.7 nM.

Formulation, Preparation, and Storage

7898-TGB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TIGIT

TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V-set and transmembrane domain-containing 3), Vsig9 (V-set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30-34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1-4). Human TIGIT cDNA encodes 244 amino acids (aa) including a 21 aa signal sequence, a 120 aa extracellular region with a V-type Ig-like domain and two potential N-glycosylation site, a 21 aa transmembrane sequence, and an 82 aa cytoplasmic domain with an ITIM motif (5). A 170 aa variant diverges after aa 166 (5). Within the ECD, human TIGIT shares only 68-75% aa sequence identity with mouse, porcine, canine, equine and bovine TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 6-8). It binds to CD155/PVR/Necl-5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1-3, 7). Binding of TIGIT by DC induces IL-10 release and inhibits IL-12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity (6-8). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 6, 7). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM-1 (6, 7). Soluble TIGIT is able to compete with DNAM-1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 8).

References

  1. Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695.
  2. Yu, X. et al. (2009) Nat. Immunol. 10:48.
  3. Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902.
  4. Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11.
  5. SwissProt Accession # Q495A1.
  6. Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
  7. Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
  8. Joller, N. et al. (2011) J. Immunol. 83:1338.

Long Name

T Cell Immunoreceptor with Ig and ITIM Domains

Alternate Names

VSIG9, VSTM3, WUCAM

Entrez Gene IDs

201633 (Human); 100043314 (Mouse); 363784 (Rat); 102121588 (Cynomolgus Monkey)

Gene Symbol

TIGIT

UniProt

AAI01289

Additional TIGIT Products

Product Documents for Recombinant Human TIGIT (A103) Fc Chimera Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human TIGIT (A103) Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human TIGIT (A103) Fc Chimera Protein, CF

Customer Reviews for Recombinant Human TIGIT (A103) Fc Chimera Protein, CF (2)

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  • Recombinant Human TIGIT Fc Chimera Protein, CF
    Name: Vijay Patel
    Application: Immunoassay Standard
    Verified Customer | Posted 12/10/2020
    Recombinant Human TIGIT (A103) Fc Chimera Protein, CF 7898-TGB
  • Recombinant Human TIGIT Fc Chimera Protein, CF
    Name: Anonymous
    Verified Customer | Posted 07/19/2020
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