Recombinant Human VEGFR2/KDR/Flk-1 Fc Avi-tag Protein, CF

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Recombinant Human VEGFR2/KDR/Flk-1 Fc Avi-tag Protein Binding Activity
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Recombinant Human VEGFR2/KDR/Flk-1 Fc Avi-tag Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF165  (Catalog # 293-VE) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human VEGFR2/KDR/Flk-1 Fc Chimera Avi-tag (Catalog # AVI10460) binds with an ED50 of 4-40 ng/mL.
Human embryonic kidney cell, HEK293-derived human VEGFR2/KDR/Flk-1 protein
Human VEGFR2/KDR/Flk-1
Accession # AAC16450.1
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer, biotinylated via Avi-tag
Predicted Molecular Mass
111 kDa
145-165 kDa, under reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human VEGFR2/KDR/Flk-1 Fc Avi-tag Protein Binding Activity View Larger

When Recombinant Human VEGF 165 (293-VE) is immobilized at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human VEGFR2/KDR/Flk-1 Fc Chimera Avi-tag (AVI10460) binds with an ED50 of 4-40 ng/mL.

SDS-PAGE Recombinant Human VEGFR2/KDR/Flk-1 Fc Avi-tag Protein SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human VEGFR2/KDR/Flk-1 Fc Chimera Avi-tag (AVI10460) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 145-165 kDa and 290-330 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

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Background: VEGFR2/KDR/Flk-1

Vascular endothelial Growth Factor Receptor 2 (VEGFR2), also known as FLK-1, KDR, and CD309, is a type I single-pass membrane receptor. Mature VEGFR2 contains a 745 amino acid extracelluar domain with seven immunoglobulin-like repeats, 21 transmembrane domain and 571 cytoplasmic domain. Within the extracellular domain, human VEGFR2 shares 80% homology with that of mouse and rat. VEGFR2, VEGFR1(Flt-1) and VEGFR3(Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors have almost exclusive expression in the endothelial cells and play essential roles in vasculogenesis and angiogenesis. VEGFR2 is the receptor for VEGF-A, VEGF-C, VEGF-D, and VEGF-E (viral homologs) (1, 2). Monomeric VEGFR2 dimerizes after binding to dimeric ligand and phosphorylate the Tyr in the cytoplasmic domain (3). VEGFR2 can also form heterodimer with VEGFR1 and VEGFR3 (4-6). Alternative splicing isoforms 2 and 3 which lack the transmembrane and cytoplasmic domains function as decoy receptors (7, 8). Targeting the signaling pathways of VEGFR1 and VEGFR2 are potential therapeutic targets for the treatment of inflammation and multiple tumors including breast, gastric, and lung carcinomas (9-12). Cancer immunotherapies using VEGF and VEGFR2 monoclonal antibodies may also be effective in combination with programmed cell death protein 1 (PD-1)/ programmed cell death ligand 1 (PD-L1) immune checkpoint blockade (13). Our Avi-tag Biotinylated VEGFR2 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

  1. Ferra, N. and Davis-Smyth, T. (1997) Endocr. Rev. 18:4.
  2. Wise, L.M. et al. (2012) Cell Microbiol. 13:1376.
  3. Lepanen, V.M. et al. (2010) Proc. Natl. Acad. Sci. USA 107:2425.
  4. Cai, M. et al. (2017) Vascul. Pharmacol. 88:11.
  5. Nilsson, I. et al. (2010) EMBO J. 29:1377.
  6. Dixelius, J. et al. (2003) J. Biol. Chem. 278:40973.
  7. Albuquerque, R.J. et al. (2009) Nat. Med. 15:1023.
  8. Jin, P. et al. (2008) Arthritis Res. Ther. 10:R73.
  9. Shibuya, M. (2015) Endocr Metab Immune Disord Drug Targets. 15:135.
  10. Farzaneh Behelgardi, M. et al. (2020), Mol Biol Rep. 47:2061.
  11. Lian, L. et al. (2019) BMC Cancer. 19:183.
  12. Hu, C. et al. (2019) Onco. Targets Ther. 12:933.
  13. Gao, F. et al. (2020) Curr. Cancer Drug Targets. 20:3.
Long Name
Vascular Endothelial Growth Factor Receptor 2
Entrez Gene IDs
3791 (Human); 16542 (Mouse)
Alternate Names
CD309 antigen; CD309; EC 2.7.10; EC; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2


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