TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF‑ beta superfamily that share a characteristic cystine knot structure (1‑7). TGF‑ beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial‑mesenchymal transition (1‑4). Each TGF‑ beta isoform has some non‑redundant functions; for TGF‑ beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation (2). Human or mouse TGF‑ beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein (8). A furin‑like convertase processes the proprotein to generate an N‑terminal 249 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 1 (8, 9). Disulfide‑linked homodimers of LAP and TGF‑ beta 1 remain non‑covalently associated after secretion, forming the small latent TGF‑ beta 1 complex (8‑10). Covalent linkage of LAP to one of three latent TGF‑ beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix (9, 10). TGF‑ beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins (10). Mature mouse TGF‑ beta 1 shares 99‑100% aa sequence identity with human, rat, equine, porcine, canine and bovine TGF‑ beta 1. It demonstrates cross‑species activity (1). TGF‑ beta 1 signaling begins with high‑affinity binding to a type II ser/thr kinase receptor termed TGF‑ beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF‑ beta RI (also called activin receptor‑like kinase (ALK) ‑5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription (3, 11, 12). Contributions of the accessory receptors betaglycan (also known as TGF‑ beta RIII) and endoglin, or use of Smad‑independent signaling pathways, allow for disparate actions observed in response to TGF‑ beta in different contexts (11).
Recombinant Mouse TGF-beta 1 Protein
R&D Systems | Catalog # 7666-MB
Key Product Details
- R&D Systems CHO-derived Recombinant Mouse TGF-beta 1 Protein (7666-MB)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Structure / Form
Applications
Product Specifications
Source
Ala279-Ser390
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 0.04-0.2 ng/mL.
Reviewed Applications
Read 14 reviews rated 5 using 7666-MB in the following applications:
Scientific Data Images for Recombinant Mouse TGF-beta 1 Protein
Bioactivity of Mouse TGF-beta 1
Recombinant mouse TGF-beta 1 (7666-MB) inhibits IL-4-dependent proliferation in the HT-2 mouse T cell line. The ED50 for this effect is 0.04-0.2 ng/mL.
Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 7666-MB
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50 μg/mL in 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Carrier Free: 7666-MB/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in HCl. |
| Reconstitution | Reconstitute at 50 μg/mL in 4 mM HCl. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: TGF-beta 1
References
- Derynck, R. and K. Miyazono (2008) Cold Spring Harbor Laboratory Press, 29.
- Dunker, N. and K. Krieglstein (2000) Eur. J. Biochem. 267:6982.
- Wahl, S.M. (2006) Immunol. Rev. 213:213.
- Chang, H. et al. (2002) Endocr. Rev. 23:787.
- Lin, J.S. et al. (2006) Reproduction 132:179.
- Hinck, A.P. et al. (1996) Biochemistry 35:8517.
- Mittl, P.R.E. et al. (1996) Protein Sci. 5:1261.
- Derynck, R. et al. (1985) Nature 316:701.
- Miyazono, K. et al. (1988) J. Biol. Chem. 263:6407.
- Oklu, R. and R. Hesketh (2000) Biochem. J. 352:601.
- de Caestecker, M. et al. (2004) Cytokine Growth Factor Rev. 15:1.
- Zuniga, J.E. et al. (2005) J. Mol. Biol. 354:1052.
Long Name
Alternate Names
Gene Symbol
UniProt
Additional TGF-beta 1 Products
Product Documents for Recombinant Mouse TGF-beta 1 Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse TGF-beta 1 Protein
For research use only
Related Research Areas
Citations for Recombinant Mouse TGF-beta 1 Protein
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Customer Reviews for Recombinant Mouse TGF-beta 1 Protein (14)
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Customer Images
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 11/17/2021
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 05/04/2021
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 12/22/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 12/02/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 03/13/2020
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Application: Media additive for protein or antibody productionVerified Customer | Posted 02/15/2020
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 03/28/2019I coated my plate with 50ng/well of recombinant mouse TGF beta 1 protein (cat# 7666-MB) overnight. I washed and blocked with reagent diluent 2 DY995 for 2hr, washed and added another company's TGFb1 antibody at different concentrations 0.2-100ug/mL in reagent diluent 2 and incubated 1hr. I did not wash my wells but add the two antibodies MAB240-biotinylated it in-house and BAF at a final concentration of 2ug/mL and incubated 1hr diluted in reagent diluent 2. I washed and added streptavidin-HRP DY998 (1:200) and incubated 30 mins, washed and developed with the substrate pack DY999. I found MAB240 competed for binding to the mouse TGF beta 1 protein when the competitor's antibody was present but the chicken polyclonal was not inhibited.
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 01/28/2019
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Application: Binding assay/Protein-protein interactionVerified Customer | Posted 06/15/2018
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 05/03/2018
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Application: Apoptosis assayVerified Customer | Posted 04/26/2018
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Application: Apoptosis assayVerified Customer | Posted 04/22/2018
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Application: Cell ProliferationVerified Customer | Posted 05/10/2017
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Application: In vivo studyVerified Customer | Posted 02/01/2017
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FAQs for Recombinant Mouse TGF-beta 1 Protein
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Q: Can I reconstitute Recombinant Mouse TGF beta 1 Protein (Catalog # 7666-MB or 7666-MB/CF) in PBS or water?
A: Recombinant Mouse TGF beta 1 Protein (Catalog # 7666-MB or 7666-MB/CF) needs to be reconstituted to 50 μg/mL in sterile 4 mM HCl containing 0.1% BSA, or 4 mM HCl. This protein is hydrophobic by nature, so it requires an acidic buffer for reconstitution to go completely into solution. Stability testing has been evaluated on the protein with these reconstitution conditions; we can guarantee performance under these reconstitution conditions. When the protein is diluted with the cell culture media or buffers to the working concentration, the concentration of the acid should be buffered or negligible. Recombinant Mouse TGF beta 1 Protein needs to be reconstituted to 50 μg/mL in sterile 4 mM HCl containing 0.1% BSA, or 4 mM HCl. This protein is hydrophobic by nature, so it requires an acidic buffer for reconstitution to go completely into solution. Stability testing has been evaluated on the protein with these reconstitution conditions; we can guarantee performance under these reconstitution conditions. When the protein is diluted with the cell culture media or buffers to the working concentration, the concentration of the acid should be buffered or negligible.
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Q: Is Recombinant Mouse TGF-beta 1 Protein (Catalog # 7666-MB) the latent or mature form?
A: Recombinant Mouse TGF-beta 1 Protein (Catalog # 7666-MB) is composed of Ala279-Ser390, accession # P04202, which corresponds to mature mouse TGFb1.
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Q: Can I reconstitute Recombinant Mouse TGF beta 1 Protein (Catalog # 7666-MB or 7666-MB/CF) in PBS or water?
A: Recombinant Mouse TGF beta 1 Protein (Catalog # 7666-MB or 7666-MB/CF) needs to be reconstituted to 50 μg/mL in sterile 4 mM HCl containing 0.1% BSA, or 4 mM HCl. This protein is hydrophobic by nature, so it requires an acidic buffer for reconstitution to go completely into solution. Stability testing has been evaluated on the protein with these reconstitution conditions; we can guarantee performance under these reconstitution conditions. When the protein is diluted with the cell culture media or buffers to the working concentration, the concentration of the acid should be buffered or negligible. Recombinant Mouse TGF beta 1 Protein needs to be reconstituted to 50 μg/mL in sterile 4 mM HCl containing 0.1% BSA, or 4 mM HCl. This protein is hydrophobic by nature, so it requires an acidic buffer for reconstitution to go completely into solution. Stability testing has been evaluated on the protein with these reconstitution conditions; we can guarantee performance under these reconstitution conditions. When the protein is diluted with the cell culture media or buffers to the working concentration, the concentration of the acid should be buffered or negligible.
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Q: Is Recombinant Mouse TGF-beta 1 Protein (Catalog # 7666-MB) the latent or mature form?
A: Recombinant Mouse TGF-beta 1 Protein (Catalog # 7666-MB) is composed of Ala279-Ser390, accession # P04202, which corresponds to mature mouse TGFb1.
Associated Pathways
Embryonic and Induced Pluripotent Stem Cell Differentiation Pathways & Lineage-specific Markers
IL-2 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-4 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-9 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
Mesenchymal Stem Cell Differentiation Pathways & Lineage-specific Markers
TGF-beta Signaling Pathways
Th17 Differentiation Pathway
