RelB Antibody (HL2222) - Azide and BSA Free

Name: RelB Antibody (HL2222) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-25623
Price: 499 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-25623

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2222 expressed in HEK293

Format

Azide and BSA Free

View all RelB Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RelB. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RelB Antibody (HL2222) - Azide and BSA Free

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] -

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (NBP3-25623) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] -

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (NBP3-25623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] -

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (NBP3-25623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] -

Western Blot: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody [HL2222] (NBP3-25623) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemistry-Paraffin: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] -

Immunohistochemistry-Paraffin: RelB Antibody (HL2222) - Azide and BSA Free [NBP3-25623] - RelB antibody [HL2222] detects RelB protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. RelB stained by RelB antibody [HL2222] (NBP3-25623) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation: RelB Antibody (HL2222) [NBP3-25623] -

Immunoprecipitation of RelB protein from Raji whole cell extract using 5 ug of RelB antibody [HL2222] (NBP3-25623).
Western blot analysis was performed using RelB antibody [HL2222] (NBP3-25623).
EasyBlot HRP-conjugated anti rabbit IgG antibody.

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Applications for RelB Antibody (HL2222) - Azide and BSA Free

Application

Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RelB

RelB, also known as I-Rel, is a member of the Rel transcription factor family which also include; RelA-p65, c-Rel, NF-kB1 p100/52 and NF-kB2 p105/50 (1). Unlike other Rel-related protein, RelB does not bind to DNA or to inhibitor IkB, but is instead sequestered in the cytoplasm by precursor p100. Once p100 is processed, the p52/RelB complex translocates into the nucleus (2). RelB is also the transcription factor that has been associated most directly with dendritic cells (DCs) differentiation and function (3). It is upregulated and translocated into the nuclei of DC in response to various stimuli. RelB is also essential for generating a specific DC subset (CD11c+/CD11b+/CD8-/Dec205-) from hematopoietic stem cells (5).

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog B

Alternate Names

IREL

Gene Symbol

RELB

Additional RelB Products

Product Documents for RelB Antibody (HL2222) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for RelB Antibody (HL2222) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.