Rictor Antibody (7B3) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80925
Key Product Details
Species Reactivity
Human, Mouse, Primate
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 7B3
Format
Azide and BSA Free
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Product Specifications
Immunogen
Purified recombinant fragment of human RICTOR expressed in E. coli. [UniProt# Q6R327]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
192 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse Rictor Antibody (7B3) - Azide and BSA Free (NBP1-51645) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Rictor Antibody (7B3) - Azide and BSA Free
![Western Blot: Rictor Antibody (7B3)Azide and BSA Free [NBP2-80925]](https://beta-resources.rndsystems.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Western-Blot-NBP2-80925-img0001.jpg "Western Blot: Rictor Antibody (7B3)Azide and BSA Free [NBP2-80925]")
Western Blot: Rictor Antibody (7B3)Azide and BSA Free [NBP2-80925]
Western Blot: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates. Image from the standard format of this antibody.![Immunocytochemistry/ Immunofluorescence: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://beta-resources.rndsystems.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80925-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
Immunocytochemistry/ Immunofluorescence: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]
Immunocytochemistry/Immunofluorescence: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Image from the standard format of this antibody.![Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://beta-resources.rndsystems.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80925-img0003.jpg "Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]
Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining. Image from the standard format of this antibody.![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://beta-resources.rndsystems.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0007.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]
Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple). Image from the standard format of this antibody.![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://beta-resources.rndsystems.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0004.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]
Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 647.![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://beta-resources.rndsystems.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0005.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]
Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://beta-resources.rndsystems.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0006.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]
Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - An intracellular stain was performed on Jurkat cells with Rictor Antibody (7B3) NBP1-51645AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.![Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]](https://beta-resources.rndsystems.com/images/products/Rictor-Antibody-7B3-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80925-img0008.jpg "Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]")
Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925]
Flow Cytometry: Rictor Antibody (7B3) - Azide and BSA Free [NBP2-80925] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using RICTOR antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.Applications for Rictor Antibody (7B3) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:10000
Flow Cytometry
1 ug per million cells
Immunocytochemistry/ Immunofluorescence
1:200-1:1000
Immunohistochemistry
1:200-1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Western Blot
1:500-1:2000
Application Notes
This RICTOR (7B3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA. The observed molecular weight of the protein in WB may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rictor
Long Name
Rapamycin-insensitive Companion of mTOR
Alternate Names
mAVO3, Pianissimo
Gene Symbol
RICTOR
Additional Rictor Products
Product Documents for Rictor Antibody (7B3) - Azide and BSA Free
Product Specific Notices for Rictor Antibody (7B3) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
mTOR Signaling Pathway
