Rictor Antibody (7B3) - BSA Free

Novus Biologicals | Catalog # NBP1-51645

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 7B3

Format

BSA Free
View all Rictor Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human RICTOR expressed in E. coli. [UniProt# Q6R327]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

192 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rictor Antibody (7B3) - BSA Free

Western Blot: Rictor Antibody (7B3)BSA Free [NBP1-51645]

Western Blot: Rictor Antibody (7B3)BSA Free [NBP1-51645]

Western Blot: Rictor Antibody (7B3) [NBP1-51645] - Western blot analysis using RICTOR mouse mAb against Hela (1), PANC-1 (2), MOLT4 (3), HepG2 (4) and HEK293 (5) cell lysates.
Immunocytochemistry/ Immunofluorescence: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Immunocytochemistry/ Immunofluorescence: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Immunocytochemistry/Immunofluorescence: Rictor Antibody (7B3) [NBP1-51645] - Analysis of NIH/3T3 cells using RICTOR mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Immunohistochemistry-Paraffin: Rictor Antibody (7B3) [NBP1-51645] - Analysis of paraffin-embedded Human breast carcinoma using Rictor antibody. Antibody dilution 1:200. Image from verified customer review
Flow Cytometry: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Flow Cytometry: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Immunohistochemistry-Paraffin: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Immunohistochemistry-Paraffin: Rictor Antibody (7B3) [NBP1-51645] - Immunohistochemical analysis of paraffin-embedded thyroid gland tissues (left) and human breast carcinoma (right) using RICTOR mouse mAb with DAB staining.
Flow Cytometry: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Flow Cytometry: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - Flow cytometric analysis of Hela cells using RICTOR mouse mAb (green) and negative control (purple).
Flow Cytometry: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Flow Cytometry: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using RICTOR antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
Flow Cytometry: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Flow Cytometry: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on Jurkat cells with Rictor Antibody (7B3) NBP1-51645AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Flow Cytometry: Rictor Antibody (7B3) - BSA Free [NBP1-51645]

Flow Cytometry: Rictor Antibody (7B3) [NBP1-51645] - An intracellular stain was performed on HeLa cells with Rictor Antibody (7B3) NBP1-51645AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Applications for Rictor Antibody (7B3) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Application Notes
This RICTOR (7B3) antibody is useful for Western blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and ELISA. The observed molecular weight of the protein in WB may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NBP1-51645 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rictor

Rictor is a binding partner of mTOR. The rictor mTOR complex modulates the phosphorylation of Protein Kinase C alpha and the actin cytoskeleton.

Long Name

Rapamycin-insensitive Companion of mTOR

Alternate Names

mAVO3, Pianissimo

Entrez Gene IDs

253260 (Human); 78757 (Mouse)

Gene Symbol

RICTOR

UniProt

Q6R327

Additional Rictor Products

Product Documents for Rictor Antibody (7B3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Rictor Antibody (7B3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rictor Antibody (7B3) - BSA Free

Customer Reviews for Rictor Antibody (7B3) - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Rictor Antibody (7B3) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Rictor Antibody (7B3)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human breast carcinoma
    Species: Human
    Verified Customer | Posted 02/09/2022
    Human breast carcinoma
    Antibody dilution 1:200
    Rictor Antibody (7B3) - BSA Free NBP1-51645

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies