Serpin E1/PAI-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-19773

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Serpin E1/PAI-1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human PAI1/Serpine 1 protein (between residues 300-400) [UniProt P05121]

Reactivity Notes

Use in Rat reported in scientific literature (PMID:35386330).

Localization

Secreted.

Specificity

PAI-1 (N315) pAb detects endogenous levels of PAI-1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Serpin E1/PAI-1 Antibody - BSA Free

Western Blot: Serpin E1/PAI-1 AntibodyBSA Free [NBP1-19773]

Western Blot: Serpin E1/PAI-1 AntibodyBSA Free [NBP1-19773]

Western Blot: Serpin E1/PAI-1 Antibody [NBP1-19773] - Extracts from Jurkat cells.
Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody - BSA Free [NBP1-19773]

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody - BSA Free [NBP1-19773]

Immunohistochemistry-Paraffin: Serpin E1/PAI-1 Antibody [NBP1-19773] - Staining of PAI1/Serpine 1 in mouse pancreas.
Western Blot: Serpin E1/PAI-1 AntibodyBSA Free [NBP1-19773]

Western Blot: Serpin E1/PAI-1 AntibodyBSA Free [NBP1-19773]

Serpin-E1-PAI-1-Antibody-Western-Blot-NBP1-19773-img0008.jpg
Immunocytochemistry/ Immunofluorescence: Serpin E1/PAI-1 Antibody - BSA Free [NBP1-19773]

Immunocytochemistry/ Immunofluorescence: Serpin E1/PAI-1 Antibody - BSA Free [NBP1-19773]

Immunocytochemistry/Immunofluorescence: Serpin E1/PAI-1 Antibody [NBP1-19773] - PAI1/Serpine1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: Serpin E1/PAI-1 AntibodyBSA Free [NBP1-19773]

Western Blot: Serpin E1/PAI-1 AntibodyBSA Free [NBP1-19773]

Serpin-E1-PAI-1-Antibody-Western-Blot-NBP1-19773-img0009.jpg
Serpin E1/PAI-1 Antibody - BSA Free

Western Blot: Serpin E1/PAI-1 Antibody - BSA Free [NBP1-19773] -

Western Blot: Serpin E1/PAI-1 Antibody - BSA Free [NBP1-19773] - (A) Immunoblots showing protein expression of ALK5, pSMAD2/3, PAI-1, pVHL, & beta -actin in A498 cells after treatment with TGF-beta at given point of time; (B) Immunoblots showing protein expression of ALK5, pSMAD2/3, PAI-1, pVHL, & beta -actin in ACHN cells after treatment with TGF-beta at given point of time; (C) Immunoblots showing protein expression of pVHL, ALK5, pSMAD2/3, PAI-1, & beta -actin after transfection of indicated vectors in A498 cells followed by TGF-beta treatment for 6 hours; (D) Immunoblots showing protein expression of pVHL, HA, pSMAD2/3, PAI-1, & beta -actin after transfection of indicated vectors in ACHN cells (48h) followed by TGF-beta treatment for 6 hours; (E) Invasion assay showing the invasiveness induced by TGF-beta in A498 cells after re-introduction of VHL (n=3 independent experiments *P< 0. 05); (F) Invasion assay showing invasiveness by TGF-beta in ACHN cells after siRNA VHL knockdown (n=3 independent experiments *P< 0.05). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.24631), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Serpin E1/PAI-1 Antibody - BSA Free

Western Blot: Serpin E1/PAI-1 Antibody - BSA Free [NBP1-19773] -

Western Blot: Serpin E1/PAI-1 Antibody - BSA Free [NBP1-19773] - (A) Immunoblots showing protein expression of ALK5, pSMAD2/3, PAI-1, pVHL, & beta -actin in A498 cells after treatment with TGF-beta at given point of time; (B) Immunoblots showing protein expression of ALK5, pSMAD2/3, PAI-1, pVHL, & beta -actin in ACHN cells after treatment with TGF-beta at given point of time; (C) Immunoblots showing protein expression of pVHL, ALK5, pSMAD2/3, PAI-1, & beta -actin after transfection of indicated vectors in A498 cells followed by TGF-beta treatment for 6 hours; (D) Immunoblots showing protein expression of pVHL, HA, pSMAD2/3, PAI-1, & beta -actin after transfection of indicated vectors in ACHN cells (48h) followed by TGF-beta treatment for 6 hours; (E) Invasion assay showing the invasiveness induced by TGF-beta in A498 cells after re-introduction of VHL (n=3 independent experiments *P< 0. 05); (F) Invasion assay showing invasiveness by TGF-beta in ACHN cells after siRNA VHL knockdown (n=3 independent experiments *P< 0.05). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.24631), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Serpin E1/PAI-1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Serpin E1/PAI-1 Antibody - BSA Free [NBP1-19773] -

Potential effect on MMT of the H-HPMA hydrogel by regulating the TGF-beta 1/Smad signal pathway. (A) Relative TGF-beta 1 mRNA expression in the peritoneal tissues; (B) the expression of TGF-beta 1, Smad2/3 and Smad7 proteins in relative to the GAPDH in the peritoneal tissue analyzed with western blotting. e,f) Representative immunofluorescence images in the rat peritoneal membrane on day 10 post-surgery. Sections were stained with DAPI (blue), PAI-1-Fluor 594 (red) and t-PA-Alexa Fluor488 (green). Magnification: 200 × ; Scale bars: 100 μm. All data are presented as mean +/- SD (n = 3 per group); the ns means no significant difference; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35386330), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Serpin E1/PAI-1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 - 10 ug/ml

Immunohistochemistry

1:50 - 1:100

Immunohistochemistry-Frozen

1:50 - 1:100

Immunohistochemistry-Paraffin

1:50 - 1:100

Western Blot

1.0 ug/ml

Reviewed Applications

Read 3 reviews rated 4.3 using NBP1-19773 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Serpin E1/PAI-1

PAI-1 and PAI-2 (for plasminogen activator inhibitor-1 and -2) are members of the serpin serine proteinase inhibitor family. PAI-1 and PAI-2 have been shown to regulate uPA (urokinase-type plasminogen activator) and tPA (tissue plasminogen activator), resulting in the inhibition of proteolytic activity. Members of the serpin family generally complex with their target proteinases, then disassociate slowly into cleaved species that fold into stable inactive forms. PAI-1 can fold into the inactive state without cleavage, resulting in the latent form of PAI-1. Activity can be restored to the latent form of PAI-1 through denaturation and renaturation. PAI-2 occurs in secreted and cytosolic forms through facultative polypeptide translocation. uPA is a serine proteinase that is a member of the trypsin family. It is responsible for the cleavage of plasminogen at the Arg-Val bond to produce plasmin. uPA consists of two chains designated A and B. The A chain can be cleaved, resulting in low and high molecular mass forms of uPA.

Long Name

Plasminogen Activator Inhibitor

Alternate Names

Nexin, PAI-1, PAI1, PLANH1

Entrez Gene IDs

5054 (Human); 18787 (Mouse); 24617 (Rat)

Gene Symbol

SERPINE1

UniProt

P05121

Additional Serpin E1/PAI-1 Products

Product Documents for Serpin E1/PAI-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Serpin E1/PAI-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Serpin E1/PAI-1 Antibody - BSA Free

Customer Reviews for Serpin E1/PAI-1 Antibody - BSA Free (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Serpin E1/PAI-1 Antibody - BSA Free
    Name: Shinford Sun
    Application: Western Blot
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 04/25/2024
    We investigated the effects of our newly designed therapeutic strategy on the cultured tumor microenvironment. The antibody was used at a 1:1000 dilution, and the bands on the Western blot were clearly visible for identification.
    Serpin E1/PAI-1 Antibody - BSA Free NBP1-19773
  • Name: Pramod Mallikarjuna
    Application: Western Blot
    Sample Tested: HEK 293 cell line vs. HEK 293 cell line treated with TGF-beta
    Species: Human
    Verified Customer | Posted 11/26/2015
    HEK 293 cell line vs. HEK 293 cell line treated with TGF-beta
    Serpin E1/PAI-1 Antibody - BSA Free NBP1-19773
  • Name: Pramod Mallikarjuna
    Application: Western Blot
    Sample Tested: Renal Cell Carcinoma tissues
    Species: Human
    Verified Customer | Posted 06/24/2014
    PAI-1 protein expression in Renal Cell Carcinoma samples.
    Serpin E1/PAI-1 Antibody - BSA Free NBP1-19773

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Protocols

View specific protocols for Serpin E1/PAI-1 Antibody - BSA Free (NBP1-19773):


Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes.
2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.


Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes.

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in wash buffer for 5 minutes.
3. Block each section with 100-400 ul blocking solution for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
7. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
8. Add 100-400 ul Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
9. Wash sections three times in wash buffer for 5 minutes each.
10. Add 100-400 ul DAB substrate to each section and monitor staining closely.
11. As soon as the sections develop, immerse slides in deionized water.
12. Counterstain sections in hematoxylin.
13. Wash sections in deionized water two times for 5 minutes each.
14. Dehydrate sections.
15. Mount coverslips.



Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot.
5. Block the membrane using standard blocking buffer for at least 1 hour.
6. Wash the membrane in wash buffer three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
8. Wash the membrane in wash buffer three times for 10 minutes each.
9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.
Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

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FAQs

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Associated Pathways

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