Q: I have just received a SHC1 antibody, and can’t find the concentration on the label. Could you please provide the concentration? The lot # is A01.

A: The concentration for NBP2-02434 Lot A01 is 1.0mg/ml.

SHC1 Antibody (OTI3A1)

Novus Biologicals | Catalog # NBP2-02434

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # OTI3A1

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Product Specifications

Immunogen

Full length human recombinant protein of human SHC1 (NP_003020) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Theoretical MW

51.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SHC1 Antibody (OTI3A1)

Western Blot: SHC1 Antibody (OTI3A1) [NBP2-02434]

Western Blot: SHC1 Antibody (OTI3A1) [NBP2-02434] - Figure from citation:(p66shc) protein level by using anti-SHC1 antibody in human HBL-100/MCF-7 cell lysates.

Immunocytochemistry/ Immunofluorescence: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunocytochemistry/Immunofluorescence: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SHC1.

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded prostate tissue using anti-SHC1 mouse monoclonal antibody.

Western Blot: SHC1 Antibody (OTI3A1) [NBP2-02434]

Western Blot: SHC1 Antibody (OTI3A1) [NBP2-02434] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHC1.

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded bladder tissue using anti-SHC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded breast tissue using anti-SHC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-SHC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-SHC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded colon tissue using anti-SHC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded Kidney tissue using anti-SHC1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434]

Immunohistochemistry-Paraffin: SHC1 Antibody (OTI3A1) [NBP2-02434] - Staining of paraffin-embedded lung tissue using anti-SHC1 mouse monoclonal antibody.

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Applications for SHC1 Antibody (OTI3A1)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SHC1

Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span

Long Name

SHC-transforming Protein 1

Alternate Names

SHCA

Entrez Gene IDs

6464 (Human)

Gene Symbol

SHC1

Additional SHC1 Products

Product Documents for SHC1 Antibody (OTI3A1)

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Certificate of Analysis

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Product Specific Notices for SHC1 Antibody (OTI3A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

PDGF Family

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SHC1 Antibody (OTI3A1)

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Q: I have just received a SHC1 antibody, and can’t find the concentration on the label. Could you please provide the concentration? The lot # is A01.

A: The concentration for NBP2-02434 Lot A01 is 1.0mg/ml.