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Simple Plex Human IL-6 3rd Gen Cartridge

Name: Simple Plex Human IL-6 3rd Gen Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-009384
Price: 885 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-009384

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Product Documents

Simple Plex Human IL-6 3rd Gen assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer. Sample type indicated with PS (Plasma/Serum).

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Saliva (25 µL), Urine (25 µL), Cerebrospinal Fluid (25 µL), Bronchoalveolar Lavage Fluid (25 µL)

Sensitivity

0.11 pg/mL

Assay Range

0.28-2652 pg/mL (Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Cerebrospinal Fluid)

Simple Plex Human IL-6 3rd Gen Cartridge Summary

Simple Plex assay for the detection of human Interleukin 6 (IL-6) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin), saliva, urine, bronchoalveolar fluid (BALF), and cerebrospinal fluid (CSF).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IL-6 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IL-6.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Bronchoalveolar Lavage Fluid, Cell Culture Supernates, Cerebrospinal Fluid, EDTA Plasma, Heparin Plasma, Saliva, Serum, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	40	40
Mean (pg/mL)	23.1	876	23.6	1008
Standard Deviation	0.899	72.1	1.82	77.7
CV%	3.9	8.2	7.7	7.7

Recovery for Simple Plex Human IL-6 3rd Gen Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	104	103-106
EDTA Plasma (n=4)	92	86-102
Heparin Plasma (n=4)	87	85-90
Saliva (n=4)	102	102-102
Serum (n=4)	94	90-99
Urine (n=4)	98	93-104

Linearity

Samples containing and/or spiked with high concentrations of human IL-6 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human IL-6 3rd Gen Cartridge

Human IL-6 (3rd gen) Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.

R&D Systems, a Bio-Techne brand, offers a comprehensive range of IL-6 Antibody products, IL-6 Proteins and Enzymes, IL-6 ELISAs, IL-6 Luminex Assays and more. Use the filters above to drill down to the IL-6 product that best fits your needs.

Long Name

Interleukin 6

Alternate Names

BSF-2, BSF2, IFNB2, IL6, MGI-2A

Entrez Gene IDs

3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)