SOX1 Antibody (JJ20-40)

Novus Biologicals | Catalog # NBP2-67864

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JJ20-40 expressed in HEK293
View all SOX1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human SOX1 aa 1-50 / 391. (SwissProt: O00570 Human; SwissProt: P53783 Mouse)

Localization

Nucleus.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOX1 Antibody (JJ20-40)

Western Blot: SOX1 Antibody (JJ20-40) [NBP2-67864]

Western Blot: SOX1 Antibody (JJ20-40) [NBP2-67864]

Western Blot: SOX1 Antibody (JJ20-40) [NBP2-67864] - Western blot analysis of SOX1 on SHG-44 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Ra
Immunocytochemistry/ Immunofluorescence: SOX1 Antibody (JJ20-40) [NBP2-67864]

Immunocytochemistry/ Immunofluorescence: SOX1 Antibody (JJ20-40) [NBP2-67864]

Immunocytochemistry/Immunofluorescence: SOX1 Antibody (JJ20-40) [NBP2-67864] - Staining SOX1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: SOX1 Antibody (JJ20-40) [NBP2-67864]

Immunohistochemistry-Paraffin: SOX1 Antibody (JJ20-40) [NBP2-67864]

Immunohistochemistry-Paraffin: SOX1 Antibody (JJ20-40) [NBP2-67864] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SOX1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemistry-Paraffin: SOX1 Antibody (JJ20-40) [NBP2-67864]

Immunohistochemistry-Paraffin: SOX1 Antibody (JJ20-40) [NBP2-67864]

Immunohistochemistry-Paraffin: SOX1 Antibody (JJ20-40) [NBP2-67864] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SOX1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
SOX1 Antibody (JJ20-40)

Western Blot: SOX1 Antibody (JJ20-40) [NBP2-67864] -

Western Blot: SOX1 Antibody (JJ20-40) [NBP2-67864] -Analysis of SOX1 on E14.0 mouse embryonic brain tissue lysates with Rabbit anti-SOX1 antibody at 1/1,000 dilution.Lysates/proteins at 30 ug/Lane.Predicted band size: 39 kDaObserved band size: 39 kDaExposure time: 3 minutes; ECL: K1802;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
SOX1 Antibody (JJ20-40)

Immunohistochemistry-Paraffin: SOX1 Antibody (JJ20-40) [NBP2-67864] -

Immunohistochemistry-Paraffin: SOX1 Antibody (JJ20-40) [NBP2-67864] - Human brain tissue with Rabbit anti-SOX1 antibody at 1/200 dilution.The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Applications for SOX1 Antibody (JJ20-40)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX1

SOX1 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. In mice, a similar protein regulates the gamma-crystallin genes and is essential for lens development.

Long Name

SRY-related HMG-box 1

Alternate Names

SRY (sex determining region Y)-box 1, SRY-related HMG-box gene 1, transcription factor SOX-1

Gene Symbol

SOX1

Additional SOX1 Products

Product Documents for SOX1 Antibody (JJ20-40)

Certificate of Analysis

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Product Specific Notices for SOX1 Antibody (JJ20-40)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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