SOX2 Antibody (CL4716) - BSA Free

Novus Biologicals | Catalog # NBP2-59057

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4716

Format

BSA Free
View all SOX2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P48431, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SOX2 Antibody (CL4716) - BSA Free

Western Blot: SOX2 Antibody (CL4716) [NBP2-59057]

Western Blot: SOX2 Antibody (CL4716) [NBP2-59057]

Western Blot: SOX2 Antibody (CL4716) [NBP2-59057] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SOX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Western Blot: SOX2 Antibody (CL4716) [NBP2-59057]

Western Blot: SOX2 Antibody (CL4716) [NBP2-59057]

Western Blot: SOX2 Antibody (CL4716) [NBP2-59057] - Analysis in human cell line NTERA-2.
Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (CL4716) [NBP2-59057]

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody (CL4716) [NBP2-59057]

Immunocytochemistry/Immunofluorescence: SOX2 Antibody (CL4716) [NBP2-59057] - Staining of U-251 cells using the anti-SOX2 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: SOX2 Antibody (CL4716) [NBP2-59057]

Immunohistochemistry-Paraffin: SOX2 Antibody (CL4716) [NBP2-59057]

Immunohistochemistry-Paraffin: SOX2 Antibody (CL4716) [NBP2-59057] - Staining of human placenta shows no positivity as expected (negative control).
Immunohistochemistry: SOX2 Antibody (CL4716) [NBP2-59057]

Immunohistochemistry: SOX2 Antibody (CL4716) [NBP2-59057]

Immunohistochemistry: SOX2 Antibody (CL4716) [NBP2-59057] - SOX2 Antibody [NBP2-59057] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing brain, eye and trigeminal ganglion.
Immunohistochemistry: SOX2 Antibody (CL4716) [NBP2-59057]

Immunohistochemistry: SOX2 Antibody (CL4716) [NBP2-59057]

Immunohistochemistry: SOX2 Antibody (CL4716) [NBP2-59057] - SOX2 Antibody [NBP2-59057] - Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing neural tube.

Applications for SOX2 Antibody (CL4716) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX2

The SOX-2 protein is a transcription factor that is critical for early embryogenesis and for embryonic stem cell pluripotency. The SOX-2 protein is also significant for eye development. SOX 2, also known as SRY related HMG BOX gene 2, belongs to the SOX (SRY-box containing gene) gene family. The SOX-2 protein is a transcription factor since it binds to DNA and regulates activity of other genes. All SOX family proteins share HMG box domains for DNA binding.

Long Name

Transcription Factor SOX2

Alternate Names

ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2

Gene Symbol

SOX2

Additional SOX2 Products

Product Documents for SOX2 Antibody (CL4716) - BSA Free

Certificate of Analysis

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Product Specific Notices for SOX2 Antibody (CL4716) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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