SOX2 Antibody (CL4716) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44099

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4716

Format

Azide and BSA Free
View all SOX2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P48431, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SOX2 Antibody (CL4716) - Azide and BSA Free

SOX2 Antibody (CL4716) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Staining of human cerebellum shows weak to moderate nuclear positivity in Bergmann glia cells.
SOX2 Antibody (CL4716) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Staining of human liver shows no positivity in hepatocytes as expected.
SOX2 Antibody (CL4716) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Staining of human skin shows moderate to strong nuclear positivity in basal cell layer.
SOX2 Antibody (CL4716) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Staining of mouse embryo E11 shows nuclear immunoreactivity in the developing spinal cord.
SOX2 Antibody (CL4716) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: SOX2 Antibody [NBP3-44099]

Immunocytochemistry/ Immunofluorescence: SOX2 Antibody [NBP3-44099]

Staining of U-251 cells using the anti-SOX2 monoclonal antibody).
SOX2 Antibody (CL4716) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Immunohistochemistry-Paraffin: SOX2 Antibody [NBP3-44099]

Staining of mouse embryo E11 shows nuclear positivity in developing brain, eye and trigeminal ganglion.
SOX2 Antibody (CL4716) - Azide and BSA Free Western Blot: SOX2 Antibody [NBP3-44099]

Western Blot: SOX2 Antibody [NBP3-44099]

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SOX2 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Applications for SOX2 Antibody (CL4716) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX2

The SOX-2 protein is a transcription factor that is critical for early embryogenesis and for embryonic stem cell pluripotency. The SOX-2 protein is also significant for eye development. SOX 2, also known as SRY related HMG BOX gene 2, belongs to the SOX (SRY-box containing gene) gene family. The SOX-2 protein is a transcription factor since it binds to DNA and regulates activity of other genes. All SOX family proteins share HMG box domains for DNA binding.

Long Name

Transcription Factor SOX2

Alternate Names

ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2

Gene Symbol

SOX2

Additional SOX2 Products

Product Documents for SOX2 Antibody (CL4716) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SOX2 Antibody (CL4716) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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