SOX9 Antibody (3C10) - Azide and BSA Free
Novus Biologicals | Catalog # H00006662-M02
![Western Blot: SOX9 Antibody (3C10) [H00006662-M02]](https://beta-resources.rndsystems.com/images/products/SOX9-Antibody-3C10-Western-Blot-H00006662-M02-img0013.jpg "Western Blot: SOX9 Antibody (3C10) [H00006662-M02]")
Loading...
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, In-situ Hybridization
Cited:
IF/IHC, In Situ Hybridization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 3C10
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
SOX9 (NP_000337, 400 a.a. ~ 509 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTRP
Marker
Sertoli Cell Marker
Specificity
SOX9 (3C10)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for SOX9 Antibody (3C10) - Azide and BSA Free
Western Blot: SOX9 Antibody (3C10) [H00006662-M02]
Western Blot: SOX9 Antibody (3C10) [H00006662-M02] - SOX9 monoclonal antibody (M02), clone 3C10 Analysis of SOX9 expression in HepG2.![Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (3C10) [H00006662-M02]](https://beta-resources.rndsystems.com/images/products/SOX9-Antibody-3C10-Immunocytochemistry-Immunofluorescence-H00006662-M02-img0008.jpg "Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (3C10) [H00006662-M02]")
Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (3C10) [H00006662-M02]
Immunocytochemistry/Immunofluorescence: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of monoclonal antibody to SOX9 on HepG2 cell. Antibody concentration 10 ug/ml.![Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02]](https://beta-resources.rndsystems.com/images/products/SOX9-Antibody-3C10-Immunohistochemistry-Paraffin-H00006662-M02-img0010.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02]")
Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02]
Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 0.7 ug/ml.![Western Blot: SOX9 Antibody (3C10) [H00006662-M02]](https://beta-resources.rndsystems.com/images/products/SOX9-Antibody-3C10-Western-Blot-H00006662-M02-img0014.jpg "Western Blot: SOX9 Antibody (3C10) [H00006662-M02]")
Western Blot: SOX9 Antibody (3C10) [H00006662-M02]
Western Blot: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody (M02), clone 3C10.Lane 1: SOX9 transfected lysate(56.1 KDa).Lane 2: Non-transfected lysate.![Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02]](https://beta-resources.rndsystems.com/images/products/SOX9-Antibody-3C10-Immunohistochemistry-Paraffin-H00006662-M02-img0006.jpg "Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02]")
Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02]
Immunohistochemistry-Paraffin: SOX9 Antibody (3C10) [H00006662-M02] - analysis of SOX9 in formalin-fixed, paraffin-embedded human fetal developing pancreas tissue using anti-SOX9 antibody. Image from verified customer review.![Immunoprecipitation: SOX9 Antibody (3C10) [H00006662-M02]](https://beta-resources.rndsystems.com/images/products/SOX9-Antibody-3C10-Immunoprecipitation-H00006662-M02-img0011.jpg "Immunoprecipitation: SOX9 Antibody (3C10) [H00006662-M02]")
Immunoprecipitation: SOX9 Antibody (3C10) [H00006662-M02]
Immunoprecipitation: SOX9 Antibody (3C10) [H00006662-M02] - Analysis of SOX9 transfected lysate using anti-SOX9 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SOX9 MaxPab rabbit polyclonal antibody.![ELISA: SOX9 Antibody (3C10) [H00006662-M02]](https://beta-resources.rndsystems.com/images/products/SOX9-Antibody-3C10-ELISA-H00006662-M02-img0015.jpg "ELISA: SOX9 Antibody (3C10) [H00006662-M02]")
ELISA: SOX9 Antibody (3C10) [H00006662-M02]
ELISA: SOX9 Antibody (3C10) [H00006662-M02] - Detection limit for recombinant GST tagged SOX9 is 0.1 ng/ml as a capture antibody.Applications for SOX9 Antibody (3C10) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
In-situ Hybridization
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. Use in Immunohistochemistry reported in scientific literature (PMID: 26030748). Use in In-situ Hybridization reported in scientific literature (PMID: 26546129).
Reviewed Applications
Read 1 review rated 5 using H00006662-M02 in the following applications:
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: SOX9
Long Name
Transcription Factor SOX9
Alternate Names
campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9
Entrez Gene IDs
6662 (Human)
Gene Symbol
SOX9
OMIM
114290 (Human)
UniProt
Additional SOX9 Products
Product Documents for SOX9 Antibody (3C10) - Azide and BSA Free
Product Specific Notices for SOX9 Antibody (3C10) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for SOX9 Antibody (3C10) - Azide and BSA Free
Powered by Bioz
Customer Reviews for SOX9 Antibody (3C10) - Azide and BSA Free (1)
5 out of 5
1 Customer Rating
Have you used SOX9 Antibody (3C10) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
1 of
1 review
Showing All
Filter By:
-
Application: Immunohistochemistry-ParaffinSample Tested: Human fetal pancreasSpecies: HumanVerified Customer | Posted 03/24/2015Human fetal developing pancreas
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
