SOX9 Antibody (5Y4J8)

Novus Biologicals | Catalog # NBP3-15875

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5Y4J8 expressed in HEK293
View all SOX9 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 169-300 of human SOX9 (P48436). HPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOX9 Antibody (5Y4J8)

Western Blot: SOX9 Antibody (5Y4J8) [NBP3-15875]

Western Blot: SOX9 Antibody (5Y4J8) [NBP3-15875]

Western Blot: SOX9 Antibody (5Y4J8) [NBP3-15875] - Western blot analysis of extracts of various cell lines, using SOX9 antibody (NBP3-15875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunoprecipitation: SOX9 Antibody (5Y4J8) [NBP3-15875]

Immunoprecipitation: SOX9 Antibody (5Y4J8) [NBP3-15875]

Immunoprecipitation: SOX9 Antibody (5Y4J8) [NBP3-15875] - Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SOX9 antibody (NBP3-15875). Western blot was performed from the immunoprecipitate using SOX9 antibody (NBP3-15875) at a dilition of 1:1000.
SOX9 Antibody (5Y4J8)

Immunohistochemistry: SOX9 Antibody (5Y4J8) [SOX9] -

Immunohistochemistry: SOX9 Antibody (5Y4J8) [SOX9] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using SOX9 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
SOX9 Antibody (5Y4J8)

Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (5Y4J8) [SOX9] -

Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (5Y4J8) [SOX9] - Confocal imaging of HCT 116 cells using SOX9 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
SOX9 Antibody (5Y4J8)

Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (5Y4J8) [SOX9] -

Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (5Y4J8) [SOX9] - Confocal imaging of paraffin-embedded Mouse testis using SOX9 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
SOX9 Antibody (5Y4J8)

Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (5Y4J8) [SOX9] -

Immunocytochemistry/ Immunofluorescence: SOX9 Antibody (5Y4J8) [SOX9] - Confocal imaging of paraffin-embedded Rat testis using SOX9 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.
SOX9 Antibody (5Y4J8)

Immunohistochemistry: SOX9 Antibody (5Y4J8) [SOX9] -

Immunohistochemistry: SOX9 Antibody (5Y4J8) [SOX9] - Immunohistochemistry analysis of paraffin-embedded Human pancreatic ductal carcinoma using SOX9 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
SOX9 Antibody (5Y4J8)

Immunohistochemistry: SOX9 Antibody (5Y4J8) [SOX9] -

Immunohistochemistry: SOX9 Antibody (5Y4J8) [SOX9] - Immunohistochemistry analysis of paraffin-embedded Human pancreas using SOX9 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
SOX9 Antibody (5Y4J8)

Immunohistochemistry: SOX9 Antibody (5Y4J8) [SOX9] -

Immunohistochemistry: SOX9 Antibody (5Y4J8) [SOX9] - Immunohistochemistry analysis of paraffin-embedded Human stomach using SOX9 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
SOX9 Antibody (5Y4J8)

Immunohistochemistry: SOX9 Antibody (5Y4J8) [SOX9] -

Immunohistochemistry: SOX9 Antibody (5Y4J8) [SOX9] - Immunohistochemistry analysis of paraffin-embedded Human colon using SOX9 Rabbit mAb at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for SOX9 Antibody (5Y4J8)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL.

Immunocytochemistry/ Immunofluorescence

1:200 - 1:2000

Immunohistochemistry

1:1000 - 1:4000

Immunohistochemistry-Paraffin

1:1000 - 1:4000

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SOX9

SOX9 is a member of the family of SOX (Sry-type highmobility group box) genes that were first identified on the basis of region with high homology to that of Sry (Sex determining region Y). SOX9 is a transcription factor with a high mobility group DNA-binding domain that is expressed in all prechondrocytic and chondrocytic cells during embryonic development in a pattern that close parallels that of the gene for type II collagen. SOX9 is important in neural crest formation, and is involved in regulating subsequent epithelial-mesenchymal transition and migration.

Long Name

Transcription Factor SOX9

Alternate Names

campomelic dysplasia, autosomal sex-reversal, CMD 1, CMD1, CMPD1, SRA1SRY (sex-determining region Y)-box 9 protein, SRY (sex determining region Y)-box 9, SRY-related HMG-box, gene 9, transcription factor SOX-9

Gene Symbol

SOX9

Additional SOX9 Products

Product Documents for SOX9 Antibody (5Y4J8)

Certificate of Analysis

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Product Specific Notices for SOX9 Antibody (5Y4J8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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