STAT1 Antibody - BSA Free

Name: STAT1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-81578
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-81578

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEVSRIVGSVE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit STAT1 Antibody - BSA Free (NBP1-81578) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for STAT1 Antibody - BSA Free

Western Blot: STAT1 Antibody [NBP1-81578]

Western Blot: STAT1 Antibody [NBP1-81578] - Analysis in human cell lines SK-MEL-30 and HEK293. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Western Blot: STAT1 Antibody [NBP1-81578]

Western Blot: STAT1 Antibody [NBP1-81578] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NBP1-81578]

Immunocytochemistry/Immunofluorescence: STAT1 Antibody [NBP1-81578] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81578]

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81578] - Staining of human skeletal muscle shows no positivity in a subset of myocytes as expected.

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81578]

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81578] - Staining of human lymph node shows cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81578]

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81578] - Staining of human kidney shows weak cytoplasmic positivity in cells in distal tubules.

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81578]

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81578] - Staining of human rectum shows moderate cytoplasmic positivity in lymphoid cells.

Chromatin Immunoprecipitation-exo-Seq: STAT1 Antibody - BSA Free [NBP1-81578]

ChIP-Exo-Seq composite graph for Anti-STAT1 (NBP1-81578) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

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Applications for STAT1 Antibody - BSA Free

Application

Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAT1

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1a and the 84 kDa STAT1b which are encoded by the same gene with splicing variant.

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Gene Symbol

STAT1

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Product Specific Notices for STAT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.