STAT1 Antibody - BSA Free

Name: STAT1 Antibody - BSA Free
Brand: Novus Biologicals
SKU: NBP1-81579
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-81579

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: FQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit STAT1 Antibody - BSA Free (NBP1-81579) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for STAT1 Antibody - BSA Free

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579]

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Analysis in human urinary bladder and skeletal muscle tissues using NBP1-81579 antibody. Corresponding STAT1 RNA-seq data are presented for the same tissues.

Western Blot: STAT1 Antibody [NBP1-81579]

Western Blot: STAT1 Antibody [NBP1-81579] - Analysis in human cell lines SK-MEL-30 and HEK293 using Anti-STAT1 antibody. Corresponding STAT1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.

Immunocytochemistry/ Immunofluorescence: STAT1 Antibody [NBP1-81579]

Immunocytochemistry/Immunofluorescence: STAT1 Antibody [NBP1-81579] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579]

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Staining of human skeletal muscle shows very weak positivity in myocytes.

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579]

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Staining of human lymph node shows cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579]

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urotheilal cells.

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579]

Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Staining of human prostate shows moderate cytoplasmic and membranous positivity in glandular cells.

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Applications for STAT1 Antibody - BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAT1

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1a and the 84 kDa STAT1b which are encoded by the same gene with splicing variant.

Long Name

Signal Transducer and Activator of Transcription 1

Alternate Names

CANDF7, ISGF-3, STAT91

Gene Symbol

STAT1

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Product Specific Notices for STAT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.