STAT5A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20505

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (93%), Porcine (92%), Rat (93%), Rhesus Macaque (97%), Sheep (93%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human STAT5A. The exact sequence is proprietary.

Localization

Cytoplasm; Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit STAT5A Antibody - BSA Free (NBP2-20505) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for STAT5A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: STAT5A Antibody [NBP2-20505]

Immunocytochemistry/ Immunofluorescence: STAT5A Antibody [NBP2-20505]

Immunocytochemistry/Immunofluorescence: STAT5A Antibody [NBP2-20505] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: STAT5A Antibody [NBP2-20505]

Immunohistochemistry-Paraffin: STAT5A Antibody [NBP2-20505]

Immunohistochemistry-Paraffin: STAT5A Antibody [NBP2-20505] - Paraffin-embedded mouse colon. STAT5A antibody [C2C3], C-term dilution: 1:250.
Western Blot: STAT5A Antibody [NBP2-20505]

Western Blot: STAT5A Antibody [NBP2-20505]

Western Blot: STAT5A Antibody [NBP2-20505] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: STAT5A Antibody [NBP2-20505]

Immunohistochemistry-Paraffin: STAT5A Antibody [NBP2-20505]

Immunohistochemistry-Paraffin: STAT5A Antibody [NBP2-20505] - araffin-embedded mouse fore brain. STAT5A antibody [C2C3], C-term dilution: 1:250.
STAT5A Antibody - BSA Free

Western Blot: STAT5A Antibody [NBP2-20505] -

Whole cell extract (22.5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A antibody [C2C3], C-term (NBP2-20505) diluted at 1:2000.
STAT5A Antibody - BSA Free

Western Blot: STAT5A Antibody [NBP2-20505] -

Whole cell extract (12.7 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A antibody [C2C3], C-term (NBP2-20505) diluted at 1:5000.

Applications for STAT5A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STAT5a

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq]

Long Name

Signal Transducer and Activator of Transcription 5

Alternate Names

MGF, signal transducer and activator of transcription 5A, STAT5

Gene Symbol

STAT5A

UniProt

Additional STAT5a Products

Product Documents for STAT5A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for STAT5A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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