INTENDED USE & DESCRIPTION

Stop Solution (Part # 895032) contains 15 vials (6 mL/vial) of 2N sulfuric acid for the stopping the action of horseradish peroxidase on the tetramethylbenzidine substrate in an ELISA.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C.

PROCEDURE

Add 50-100 µL/well to stop the action of horseradish peroxidase on the substrate, tetramethylbenzidine. If the color in the wells is green or the color change does not appear to be uniform, gently tap the plate to ensure thorough mixing.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

  • Caution: Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use.
Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10

  1. Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation
    Authors: Elodie Weider
    J Biol Chem, 2016;0(0):. 2016
  2. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
    Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
    PLoS ONE, 2015;10(1):e0116772. 2015
  3. The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1.
    Authors: Guzzo C, Fox J, Lin Y, Miao H, Cimbro R, Volkman B, Fauci A, Lusso P
    PLoS Pathog, 2013;9(12):e1003852. 2013
  4. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010;5(2):e9246. 2010
  5. First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
    Authors: Thadhani R, Powe CE, Tjoa ML
    Diabetes Care, 2010;33(3):664-9. 2010
  6. Endothelin-converting enzyme-2 is increased in Alzheimer's disease and up-regulated by Abeta.
    Authors: Palmer JC, Baig S, Kehoe PG, Love S
    Am. J. Pathol., 2009;175(1):262-70. 2009
  7. Up-regulated macrophage migration inhibitory factor protects apoptosis of dermal fibroblasts in patients with systemic sclerosis.
    Authors: Kim JY, Kwok SK, Hur KH, Kim HJ, Kim NS, Yoo SA, Kim WU, Cho CS
    Clin. Exp. Immunol., 2008;152(2):328-35. 2008
  8. Nanobacteria are mineralo fetuin complexes.
    Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
    PLoS Pathog., 2008;4(2):e41. 2008
  9. Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
    Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM
    Chest, 2007;132(1):58-63. 2007
  10. Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution.
    Authors: Kugananthan S, Lai C, Gridneva Z, Mark P, Geddes D, Kakulas F
    Nutrients, 0;8(11):. 0
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Average Rating: 4.7 (Based on 3 reviews)

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Other Experimental DetailsPerfect for stopping the action of HRP on TMB in ELISA
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