Stop Solution 2N Sulfuric Acid (15 x 6 mL) (DY994): R&D Systems

INTENDED USE & DESCRIPTION

Stop Solution (Part # 895032) contains 15 vials (6 mL/vial) of 2N sulfuric acid for the stopping the action of horseradish peroxidase on the tetramethylbenzidine substrate in an ELISA.

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C.

PROCEDURE

Add 50-100 µL/well to stop the action of horseradish peroxidase on the substrate, tetramethylbenzidine. If the color in the wells is green or the color change does not appear to be uniform, gently tap the plate to ensure thorough mixing.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

  • Caution: Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use.
Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation
    Authors: Elodie Weider
    J Biol Chem, 2016;0(0):. 2016
  2. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
    Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
    PLoS ONE, 2015;10(1):e0116772. 2015
  3. The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1.
    Authors: Guzzo C, Fox J, Lin Y, Miao H, Cimbro R, Volkman B, Fauci A, Lusso P
    PLoS Pathog, 2013;9(12):e1003852. 2013
  4. Clearance and toxicity of recombinant methionyl human glial cell line-derived neurotrophic factor (r-metHu GDNF) following acute convection-enhanced delivery into the striatum.
    Authors: Taylor H, Barua N, Bienemann A, Wyatt M, Castrique E, Foster R, Luz M, Fibiger C, Mohr E, Gill S
    PLoS ONE, 2013;8(3):e56186. 2013
  5. First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
    Authors: Thadhani R, Powe CE, Tjoa ML
    Diabetes Care, 2010;33(3):664-9. 2010
  6. Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
    Authors: Khankin EV, Mutter WP, Tamez H
    PLoS ONE, 2010;5(2):e9246. 2010
  7. Endothelin-converting enzyme-2 is increased in Alzheimer's disease and up-regulated by Abeta.
    Authors: Palmer JC, Baig S, Kehoe PG, Love S
    Am. J. Pathol., 2009;175(1):262-70. 2009
  8. Up-regulated macrophage migration inhibitory factor protects apoptosis of dermal fibroblasts in patients with systemic sclerosis.
    Authors: Kim JY, Kwok SK, Hur KH, Kim HJ, Kim NS, Yoo SA, Kim WU, Cho CS
    Clin. Exp. Immunol., 2008;152(2):328-35. 2008
  9. Nanobacteria are mineralo fetuin complexes.
    Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
    PLoS Pathog., 2008;4(2):e41. 2008
  10. Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
    Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM
    Chest, 2007;132(1):58-63. 2007
  11. Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution.
    Authors: Kugananthan S, Lai C, Gridneva Z, Mark P, Geddes D, Kakulas F
    Nutrients, 0;8(11):. 0
Expand to show all 11 Citations
Supplemental ELISA Products
Description Application Cat# Citations Images  

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)

ELISA DY999 55
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Reagent Diluent Concentrate 2 (10X, 5 x 21 mL)

ELISA DY995 14
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

ELISA Plate Sealers, 100 Pack

ELISA DY992
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Streptavidin-HRP (set of 5 vials enough for 75 plates total)

ELISA DY998 44
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Quantikine Wash Buffer 1

ELISA WA126 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Clear Polystyrene Microplates, 25 Pack

ELISA DY990 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Glo Substrate Reagent Pack (Reagent A & B)

ELISA DY993 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Cell Lysis Buffer 2 (1 x 21 mL)

ELISA 895347 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Reagent Diluent Concentrate 1 (21 mL)

ELISA DY997 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sample Diluent Concentrate 2 (2X)

ELISA DYC002 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Reagent Diluent Concentrate 3 (5X, 10 x 21 mL)

ELISA DY004
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Sample Diluent Concentrate 1 (5X, 5 x 21 mL)

ELISA DYC001 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Cell Lysis Buffer 1 (1 x 21 mL)

ELISA 890713 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Black Polystyrene Microplates, 25 Pack

ELISA DY991 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Cell Lysis Buffer 5 (1 x 21 mL)

ELISA 895890
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Cell Lysis Buffer 3 Concentrate (5X, 1 x 21 mL)

ELISA 895366
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 4.8 (Based on 6 reviews)

Have you used Stop Solution 2N Sulfuric Acid (15 x 6 mL)?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Stop Solution 2N Sulfuric Acid (15 x 6 mL).

Filter by Ratings
Excellent
(5)
Very Good
(1)
All Ratings
Filter by Applications
All Applications
Filter by Species
All Species

Images Ratings Applications Species Reviewed By Date Details
 Stop Solution 2N Sulfuric Acid (15 x 6 mL) DY994
Enlarge
Excellent
  Anonymous 07/19/2017
Show
Image Details
 Stop Solution 2N Sulfuric Acid (15 x 6 mL) DY994
: Stop Solution 2N Sulfuric Acid (15 x 6 mL) [DY994]

Other Experimental Details

Other Experimental DetailsUsed stop solution to read the plate and it works perfect
 Stop Solution 2N Sulfuric Acid (15 x 6 mL) DY994
Enlarge
Excellent
  Anonymous 07/14/2017
Show
Image Details
 Stop Solution 2N Sulfuric Acid (15 x 6 mL) DY994
: Stop Solution 2N Sulfuric Acid (15 x 6 mL) [DY994]
  Excellent
  Anonymous 07/06/2017
Show
  Excellent
  Anonymous 03/29/2017
Show

Other Experimental Details

Other Experimental DetailsPerfect for stopping the action of HRP on TMB in ELISA
  Very Good
  Anonymous 01/18/2016
Show
  Excellent
  Anonymous 01/10/2016
Show

Order Details

Contact Information

R&D Systems Guarantee

Feedback

Customer Information