Survivin Antibody Pack

Novus Biologicals | Catalog # NB100-911

Novus Biologicals
100% Guarantee

Key Product Details

Species

Human, Mouse

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Product Summary for Survivin Antibody Pack

This pack contains 1 vial each of: NB500-201 (0.1 mL), NB500-237 (0.1 mL), NB500-238 (0.1 mL), HAF007 and HAF008.

Product Specifications

Application Notes

See individual datasheets of components for their validated applications

Reactivity Notes

See individual datasheets of components for their validated species

Marker

Astrocyte Marker

Scientific Data Images for Survivin Antibody Pack

Dual RNAscope ISH-IHC: Survivin Antibody Pack [NB100-911] - Formalin-fixed paraffin-embedded tissue sections of human esophagus squamous cell carcinoma were probed for Survivin mRNA (ACD RNAScope Probe, [465361]; Fast Red chromogen, ACD [322360]). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal [NB500-201] at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody [VC003] and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to tumor cells.
Knockdown Validated: Survivin Antibody Pack [NB100-911]

Immunocytochemistry/ Immunofluorescence: Survivin Antibody Pack [NB100-911]

Survivin-Antibody-Pack-Knockdown-Validated-NB100-911-img0008.jpg
Knockdown Validated: Survivin Antibody Pack [NB100-911]

Western Blot: Survivin Antibody Pack [NB100-911]

Western Blot: Survivin Antibody Pack [NB100-911] - Suppression of survivin expression by transfection of siRNA. RNA interference was performed in SW1353 and Hs 819.T, either for GFP as control or for survivin. A: A pronounced decrease of survivin protein levels was measured by immunoblotting in SW1353 and Hs819.T. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-11-120), licensed under a CC-BY license. Survivin Antibody (60.11) [NB500-238]
Immunocytochemistry/ Immunofluorescence: Survivin Antibody Pack [NB100-911]

Immunocytochemistry/ Immunofluorescence: Survivin Antibody Pack [NB100-911]

Immunocytochemistry/Immunofluorescence: Survivin Antibody Pack [NB100-911] - Analysis using the HRP conjugate of [NB500-201]. Staining of Telophase with accumulation of survivin in the midbodies of two daughter cells. Survivin detection using [NB500-201].
Immunohistochemistry: Survivin Antibody Pack [NB100-911]

Immunohistochemistry: Survivin Antibody Pack [NB100-911]

Survivin-Antibody-Pack-Immunohistochemistry-NB100-911-img0005.jpg
Immunohistochemistry-Paraffin: Survivin Antibody Pack [NB100-911]

Immunohistochemistry-Paraffin: Survivin Antibody Pack [NB100-911]

Immunohistochemistry-Paraffin: Survivin Antibody Pack [NB100-911] - Immunohistochemical staining of formalin-fixed paraffin-embedded human lung cancer tissue using Survivin Antibody (32.1) [NB500-237].

Formulation, Preparation, and Storage

Concentration

Concentration of individual antibodies may be found on the vial label. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Survivin

Survivin (BIRC5 or IAP4) is the smallest member of the inhibitor of apoptosis (IAP) proteins, containing only a single baculovirus IAP repeat (BIR) domain and lacking the C-terminal RING domain. Survivin has a theoretical molecular weight of 16.5 kDa (wild-type protein) and exists as ten splice variant forms including the most predominant isoforms, survivin-2B and surviving-DeltaEx3 (1). It also undergoes phosphorylation by PKA, Plk1, Cdk1, CKII and Aurora B at multiple sites (e.g. Serine 20 and Threonine 34, 53 and 117).

Besides being highly abundant in fetal development and expressed in proliferating adult cells such as activated T lymphocytes, erythroblasts, and self-renewing stem cells, survivin is generally absent in adult tissues. However, it is elevated in common cancers such as lung, colon, pancreas, breast and prostate where it drives proliferation, metastasis, poor prognosis, and decreased patient survival (2).

Survivin has been shown to be involved in multiple cellular processes including cell cycle progression, mitotic spindle assembly, kinetochore attachment, angiogenesis, migration, and its anti-apoptotic activity has been linked to both its monomeric and homodimeric forms. Survivin impacts the function of other IAP members, c-IAP1 and c-IAP-2, or modulates the inhibitory activity of XIAP against caspases by forming a stable complex with XIAP and HBXIP. During the intrinsic apoptotic pathway, survivin may prevent the release of mitochondrial APAF1 into the cytoplasm or hinder the association of SMAC with other IAPS, which results in prolonged cell survival (3).

References

1. Sah NK, Seniya C. (2015) Survivin splice variants and their diagnostic significance. Tumour Biol. 36(9):6623-31. PMID: 26245993

2. Lladser A, Sanhueza C, Kiessling R, Quest AF. (2011) Is survivin the potential Achilles' heel of cancer? Adv Cancer Res. 111:1-37. PMID: 21704829

3. Wheatley SP, Altieri DC. (2019) Survivin at a glance. J Cell Sci. 132(7). PMID: 30948431

Alternate Names

API4, BIRC5

Gene Symbol

BIRC5

Additional Survivin Products

Product Documents for Survivin Antibody Pack

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Survivin Antibody Pack

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Packs are guaranteed for 1 year from date of receipt.

Citations for Survivin Antibody Pack

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Survivin Antibody Pack

Showing  1 - 4 of 4 FAQs Showing All

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: What does this "pack" come with?

    A: This Survivin Antibody Pack comes with 1 vial each of: NB500-201 (0.1ml), NB500-237 (0.1ml) and NB500-238 (0.1ml). These are all Survivin antibodies.

  • Q: What is the concentration of these antibodies?

    A: NB500-238 has a concentration of 0.9 mg/ml, NB500-201 has a concentration of 1 mg/ml, and NB500-
    237 has a concentration of 1.9 mg/ml.

  • Q: What is the host, clonality, and clone numbers of the antibodies contained in the pack?

    A: NB500-238 is a mouse monoclonal ab with clone number (60.11);  NB500-201 is a rabbit polyclonal ab, clone number is not applicable; NB500-
    237 is a mouse monoclonal ab with clone number (32.1).

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: What does this "pack" come with?

    A: This Survivin Antibody Pack comes with 1 vial each of: NB500-201 (0.1ml), NB500-237 (0.1ml) and NB500-238 (0.1ml). These are all Survivin antibodies.

  • Q: What is the concentration of these antibodies?

    A: NB500-238 has a concentration of 0.9 mg/ml, NB500-201 has a concentration of 1 mg/ml, and NB500-
    237 has a concentration of 1.9 mg/ml.

  • Q: What is the host, clonality, and clone numbers of the antibodies contained in the pack?

    A: NB500-238 is a mouse monoclonal ab with clone number (60.11);  NB500-201 is a rabbit polyclonal ab, clone number is not applicable; NB500-
    237 is a mouse monoclonal ab with clone number (32.1).

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: What does this "pack" come with?

    A: This Survivin Antibody Pack comes with 1 vial each of: NB500-201 (0.1ml), NB500-237 (0.1ml) and NB500-238 (0.1ml). These are all Survivin antibodies.

  • Q: What is the concentration of these antibodies?

    A: NB500-238 has a concentration of 0.9 mg/ml, NB500-201 has a concentration of 1 mg/ml, and NB500-
    237 has a concentration of 1.9 mg/ml.

  • Q: What is the host, clonality, and clone numbers of the antibodies contained in the pack?

    A: NB500-238 is a mouse monoclonal ab with clone number (60.11);  NB500-201 is a rabbit polyclonal ab, clone number is not applicable; NB500-
    237 is a mouse monoclonal ab with clone number (32.1).

  • Q: Can I use this antibody with species other than those listed?

    A: The species we have listed are validated and therefore have a 100% guarantee to work with this antibody. We cannot guarantee that this will work with other species.

  • Q: What does this "pack" come with?

    A: This Survivin Antibody Pack comes with 1 vial each of: NB500-201 (0.1ml), NB500-237 (0.1ml) and NB500-238 (0.1ml). These are all Survivin antibodies.

  • Q: What is the concentration of these antibodies?

    A: NB500-238 has a concentration of 0.9 mg/ml, NB500-201 has a concentration of 1 mg/ml, and NB500-
    237 has a concentration of 1.9 mg/ml.

  • Q: What is the host, clonality, and clone numbers of the antibodies contained in the pack?

    A: NB500-238 is a mouse monoclonal ab with clone number (60.11);  NB500-201 is a rabbit polyclonal ab, clone number is not applicable; NB500-
    237 is a mouse monoclonal ab with clone number (32.1).

Showing  1 - 4 of 4 FAQs Showing All
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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail