Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TEM8/ANTXR1 Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 13 amino acids near the center of human ATR. The immunogen is located within amino acids 220 - 270 of ATR.

Specificity

Recognizes all three isoforms of ATR.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TEM8/ANTXR1 Antibody - BSA Free

Western Blot: TEM8/ANTXR1 AntibodyBSA Free [NBP1-77233]

Western Blot: TEM8/ANTXR1 AntibodyBSA Free [NBP1-77233]

Western Blot: TEM8/ANTXR1 Antibody [NBP1-77233] - HepG2 cell lysates with ATR antibody (IN) at (A) 0.5, (B) 1, and (C) 2 ug/ml.
TEM8/ANTXR1 Antibody - BSA Free

Immunohistochemistry: TEM8/ANTXR1 Antibody - BSA Free [NBP1-77233] -

Immunohistochemistry: TEM8/ANTXR1 Antibody - BSA Free [NBP1-77233] - Immunohistochemical staining of human brain tissue using TEM8/ANTXR1 antibody at 2 u/mL.
TEM8/ANTXR1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TEM8/ANTXR1 Antibody - BSA Free [NBP1-77233] -

Immunocytochemistry/ Immunofluorescence: TEM8/ANTXR1 Antibody - BSA Free [NBP1-77233] - Immunofluorescence of TEM8/ANTXR1 in Human Brain tissue with TEM8/ANTXR1 antibody at 10 ug/mL.

Applications for TEM8/ANTXR1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2 ug/ml

Immunohistochemistry-Paraffin

2 ug/ml

Western Blot

0.5-2 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TEM8/ANTXR1

The Anthrax toxin receptor (ATR) was initially discovered as the tumor endothelial marker 8 (TEM8). This protein, which exists in three isoforms (36, 40, and 60 kDa), is highly expressed in tumor vessels as well as in the vasculature of developing embryos, suggesting that it may normally play a role in angiogenesis. However, it also acts as the receptor for anthrax toxin. Following the binding of this protein by the protective antigen (PA) of anthrax, PA is cleaved and heptamerizes to form the binding site for both edema factor (EF) and lethal factor (LF). This complex is then endocytosed by the cell; acidification in endosomes allows the release of EF and LF into the cytoplasm where they interfere with MAPK signaling and induce apoptosis.

Long Name

Tumor Endothelial Marker 8/Anthrax Toxin Receptor 1

Alternate Names

ANTXR1

Entrez Gene IDs

84168 (Human); 69538 (Mouse); 362393 (Rat)

Gene Symbol

ANTXR1

UniProt

Q9H6X2

Additional TEM8/ANTXR1 Products

Product Documents for TEM8/ANTXR1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TEM8/ANTXR1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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