TGF‑ beta Pan Specific Antibody

R&D Systems | Catalog # AB-100-NA

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Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human, Mouse, Rat, Bovine, Goat, Mink, Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio hamadryas (Hamadryas Baboon), Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Immunocytochemistry, Bioassay, Blocking, Cell Culture, In vivo assay, Electron Microscopy

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TGF-beta Primary Antibodies »

Product Specifications

Immunogen

Recombinant human TGF‑ beta 1, porcine platelet-derived TGF‑ beta 1.2, porcine platelet-derived TGF‑ beta 2, and recombinant amphibian TGF‑ beta 5

Specificity

Detects TGF-beta 1, TGF-beta 1.2, TGF-beta 2, TGF-beta 3, and TGF-beta 5 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for TGF‑ beta Pan Specific Antibody

TGF-beta Pan Specific antibody in Human Prostate Tissue by Immunohistochemistry (IHC-P).

TGF-beta Pan Specific in Human Prostate Tissue.

TGF-beta was detected in immersion fixed paraffin-embedded sections of human prostate tissue using Rabbit Anti-TGF-beta Pan Specific Polyclonal Antibody (Catalog # AB-100-NA) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epitjhelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta Antibody.

TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta Antibody.

Porcine TGF-beta 1 (Catalog # 101-B1) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Porcine TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of TGF-beta Pan Specific Polyclonal Antibody (Catalog # AB-100-NA). The ND50range is 5-30 µg/mL.
Detection of Human TGF-beta by Flow Cytometry

Detection of Human TGF-beta by Flow Cytometry

TGF-beta induces IL-9 mRNA expression in Th1 and Th2 cells.Naïve CD4+ T cells were activated with fibroblast-bound anti-CD3/CD28 under classical Th1 and Th2 conditions, with IL-12 and anti-IL-4 or IL-4, anti-IFN-gamma, and anti-IL-12 respectively for 5 days, restimulated at day 5 and split in cultures with and without TGF-beta or anti-TGF-beta for 5 more days of stimulation. (A, C and D) qRT-PCR gene expression analysis of the relative gene expression of IL-9, GATA3, and Tbet at day 10 relative to the Th0 culture. (B) CT value from qRT-PCR gene expression analysis of the gene PU.1. Each donor is represented by a specific symbol and connected with a line. Data are from four independent experiments, each with two donors. *p<0.05, **p<0.01, ***p<0.001. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0021695), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for TGF‑ beta Pan Specific Antibody

Application
Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate tissue

Western Blot

1 µg/mL
Sample: Recombinant Human TGF-beta 1 (Catalog # 240-B)
Recombinant Human TGF-beta 2 (Catalog # 302-B2)
Recombinant Human TGF-beta 1.2 (Catalog # 304-B3)
Recombinant Human TGF-beta 3 (Catalog # 243-B3)
Recombinant Amphibian TGF-beta 5 (Catalog # 245-B5)
under non-reducing conditions only

Neutralization

Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is approximately  1 µg/mL, 15 µg/mL, 4 µg/mL, and 1 µg/mL for Porcine TGF-beta 1.2, Porcine TGF-beta 2, Recombinant Chicken TGF-beta 3, and Recombinant Amphibian TGF-beta 5, respectively. The ND50 range for Porcine TGF-beta 1 is 5-30 µg/mL.

Reviewed Applications

Read 3 reviews rated 4.3 using AB-100-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TGF-beta

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.

Long Name

Transforming Growth Factor beta

Alternate Names

TGFbeta

Additional TGF-beta Products

Product Documents for TGF‑ beta Pan Specific Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for TGF‑ beta Pan Specific Antibody

For research use only

Citations for TGF‑ beta Pan Specific Antibody

Customer Reviews for TGF‑ beta Pan Specific Antibody (3)

4.3 out of 5
3 Customer Ratings
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Customer Images

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  • TGF-beta Pan Specific Antibody
    Name: Tina Yuan
    Application: Immunohistochemistry
    Sample Tested: mouse oral tissue
    Species: Mouse
    Verified Customer | Posted 11/23/2020
    TGF‑ beta Pan Specific Antibody AB-100-NA
  • TGF-beta Pan Specific Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 03/23/2018
  • TGF-beta Pan Specific Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Peritoneal macrophages
    Species: Mouse
    Verified Customer | Posted 06/06/2017

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TGF‑ beta Pan Specific Antibody

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  • Q: Does TGF-beta Pan Specific Antibody, catalog # AB-100-NA, recognize active or latent TGF-beta (LAP)?

    A: This antibody recognizes mature (active) TGF-beta. We have not tested for cross-reactivity to LAP (catalog # 246-LP) in our neutralization assay. Some cross-reactivity to latent TGF-beta may also be observed in western blot.

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Associated Pathways

Th17 Differentiation Pathway Th17 Differentiation Pathway Thumbnail