TGF-beta Pan Specific Antibody

(43 citations)
(1 Review)
  
  • Specificity
    Detects TGF-beta 1, TGF-beta 1.2, TGF-beta 2, TGF-beta 3, and TGF-beta 5 in direct ELISAs and Western blots.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Protein A or G purified
  • Immunogen
    Recombinant human TGF‑ beta 1, porcine platelet-derived TGF‑ beta 1.2, porcine platelet-derived TGF‑ beta 2, and recombinant amphibian TGF‑ beta 5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human TGF-beta 1 (Catalog # 240-B)
    Recombinant Human TGF-beta 2 (Catalog # 302-B2)
    Recombinant Human TGF-beta 1.2 (Catalog # 304-B3)
    Recombinant Human TGF-beta 3 (Catalog # 243-B3)
    Recombinant Amphibian TGF-beta 5 (Catalog # 245-B5)
    under non-reducing conditions only
  • Neutralization
    Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line. Tsang, M. et al. (1995) Cytokine 7:389. The Neutralization Dose (ND50) is approximately  1 µg/mL, 15 µg/mL, 4 µg/mL, and 1 µg/mL for Porcine TGF-beta 1.2, Porcine TGF-beta 2, Recombinant Chicken TGF-beta 3, and Recombinant Amphibian TGF-beta 5, respectively. The ND50 range for Porcine TGF-beta 1 is 5-30 µg/mL.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
TGF‑ beta 1 Inhibition of IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta Antibody. Porcine TGF-beta 1 (Catalog # 101-B1) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse
T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4
(7.5 ng/mL) activity elicited by Porcine TGF-beta 1 (1 ng/mL) is neutralized (green line) by increasing concentrations of TGF-beta  Pan Specific Polyclonal Antibody (Catalog # AB-100-NA). The ND50 range is 5-30  µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 1 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TGF-beta

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.

  • Long Name:
    Transforming Growth Factor beta
  • Alternate Names:
    TGFbeta; TGF-beta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

43 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-? Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation
    Authors: N Selvamurug, Z He, D Rifkin, B Dabovic, NC Partridge
    Stem Cells Int, 2017;2017(0):2450327.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Nonmyocyte ERK1/2 signaling contributes to load-induced cardiomyopathy in Marfan mice
    Authors: R Rouf, EG MacFarlane, E Takimoto, R Chaudhary, V Nagpal, PP Rainer, JG Bindman, EE Gerber, D Bedja, C Schiefer, KL Miller, G Zhu, L Myers, N Amat-Alarc, DI Lee, N Koitabashi, DP Judge, DA Kass, HC Dietz
    JCI Insight, 2017;2(15):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  3. ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury
    Authors: Y Yang, H Liu, H Zhang, Q Ye, J Wang, B Yang, L Mao, W Zhu, RK Leak, B Xiao, B Lu, J Chen, X Hu
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  4. Endoplasmic Reticulum Oxidative Stress Triggers Tgf-Beta-Dependent Muscle Dysfunction by Accelerating Ascorbic Acid Turnover
    Authors: D Pozzer, M Favellato, M Bolis, RW Invernizzi, F Solagna, B Blaauw, E Zito
    Sci Rep, 2017;7(0):40993.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. MXRA5 is a TGF-?1-regulated human protein with anti-inflammatory and anti-fibrotic properties
    J Cell Mol Med, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. TGF-? Neutralization Enhances AngII-Induced Aortic Rupture and Aneurysm in Both Thoracic and Abdominal Regions
    Authors: X Chen, DL Rateri, DA Howatt, A Balakrishn, JJ Moorleghen, LA Cassis, A Daugherty
    PLoS ONE, 2016;11(4):e0153811.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  7. Lymphatic vessels regulate immune microenvironments in human and murine melanoma
    J Clin Invest, 2016;0(0):.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  8. Osmotic Induction of Angiogenic Growth Factor Expression in Human Retinal Pigment Epithelial Cells.
    Authors: Veltmann M, Hollborn M, Reichenbach A, Wiedemann P, Kohen L, Bringmann A
    PLoS ONE, 2016;11(1):e0147312.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. Bmi1 limits dilated cardiomyopathy and heart failure by inhibiting cardiac senescence.
    Authors: Gonzalez-Valdes I, Hidalgo I, Bujarrabal A, Lara-Pezzi E, Padron-Barthe L, Garcia-Pavia P, Gomez-del Arco P, Redondo J, Ruiz-Cabello J, Jimenez-Borreguero L, Enriquez J, de la Pompa J, Hidalgo A, Gonzalez S
    Nat Commun, 2015;6(0):6473.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  10. Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts.
    Authors: Angelucci C, Maulucci G, Colabianchi A, Iacopino F, D'Alessio A, Maiorana A, Palmieri V, Papi M, De Spirito M, Di Leone A, Masetti R, Sica G
    Br J Cancer, 2015;112(10):1675-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  11. Identification of genetic loci that control mammary tumor susceptibility through the host microenvironment.
    Authors: Zhang P, Lo A, Huang Y, Huang G, Liang G, Mott J, Karpen G, Blakely E, Bissell M, Barcellos-Hoff M, Snijders A, Mao J
    Sci Rep, 2015;5(0):8919.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Blocking
  12. CD4(+)Foxp3(+) Tregs protect against innate immune cell-mediated fulminant hepatitis in mice.
    Authors: Hou X, Song J, Su J, Huang D, Gao W, Yan J, Shen J
    Mol Immunol, 2015;63(2):420-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  13. Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation.
    Authors: Zhu L, Huang Z, Stalesen R, Hansson G, Li N
    J Thromb Haemost, 2014;12(7):1156-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  14. Autophagy fosters myofibroblast differentiation through MTORC2 activation and downstream upregulation of CTGF.
    Authors: Bernard M, Dieude M, Yang B, Hamelin K, Underwood K, Hebert M
    Autophagy, 2014;10(12):2193-207.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  15. Expression of versican V3 by arterial smooth muscle cells alters tumor growth factor beta (TGFbeta)-, epidermal growth factor (EGF)-, and nuclear factor kappaB (NFkappaB)-dependent signaling pathways, creating a microenvironment that resists monocyte adhesion.
    Authors: Kang I, Yoon D, Braun K, Wight T
    J Biol Chem, 2014;289(22):15393-404.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  16. Loss of Dab2 expression in breast cancer cells impairs their ability to deplete TGF-beta and induce Tregs development via TGF-beta.
    Authors: Xu S, Zhu J, Wu Z
    PLoS ONE, 2014;9(3):e91709.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  17. Emdogain-regulated gene expression in palatal fibroblasts requires TGF-betaRI kinase signaling.
    Authors: Stahli A, Bosshardt D, Sculean A, Gruber R
    PLoS ONE, 2014;9(9):e105672.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  18. IL-2/anti-IL-2 complex attenuates renal ischemia-reperfusion injury through expansion of regulatory T cells.
    Authors: Kim M, Koo T, Yan J, Lee E, Han K, Jeong J, Ro H, Kim B, Jo S, Oh K, Surh C, Ahn C, Yang J
    J Am Soc Nephrol, 2013;24(10):1529-36.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  19. Transglutaminase 2 cross-linking activity is linked to invadopodia formation and cartilage breakdown in arthritis.
    Authors: Lauzier A, Charbonneau M, Paquette M, Harper K, Dubois CM
    Arthritis Res. Ther., 2012;14(4):R159.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  20. Toll-like Receptor 2 (TLR2), Transforming Growth Factor-beta, Hyaluronan (HA), and Receptor for HA-mediated Motility (RHAMM) Are Required for Surfactant Protein A-stimulated Macrophage Chemotaxis.
    Authors: Foley J, Lam D, Jiang H, Liao J, Cheong N, McDevitt T, Zaman A, Wright J, Savani R
    J Biol Chem, 2012;287(44):37406-19.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  21. Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
    Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D
    J Immunol, 2012;189(12):5590-601.
    Species: Human
    Sample Type: In Vivo
    Application: Bioassay
  22. Nonmyelinating Schwann cells maintain hematopoietic stem cell hibernation in the bone marrow niche.
    Authors: Yamazaki S, Ema H, Karlsson G, Yamaguchi T, Miyoshi H, Shioda S, Taketo MM, Karlsson S, Iwama A, Nakauchi H
    Cell, 2011;147(5):1146-58.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  23. Calpain mediates pulmonary vascular remodeling in rodent models of pulmonary hypertension, and its inhibition attenuates pathologic features of disease.
    Authors: Ma W, Han W, Greer PA, Tuder RM, Toque HA, Wang KK, Caldwell RW, Su Y
    J. Clin. Invest., 2011;121(11):4548-66.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  24. IL-33 induces IL-9 production in human CD4+ T cells and basophils.
    Authors: Blom L, Poulsen BC, Jensen BM, Hansen A, Poulsen LK
    PLoS ONE, 2011;6(7):e21695.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  25. The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
    Authors: Suzuki K, Maruya M, Kawamoto S
    Immunity, 2010;33(1):71-83.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  26. Interferon-gamma and the interferon-inducible chemokine CXCL10 protect against aneurysm formation and rupture.
    Authors: King VL, Lin AY, Kristo F, Anderson TJ, Ahluwalia N, Hardy GJ, Owens AP, Howatt DA, Shen D, Tager AM, Luster AD, Daugherty A, Gerszten RE
    Circulation, 2009;119(3):426-35.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  27. Molecular mechanisms of TGFbeta receptor-triggered signaling cascades rapidly induced by the calcineurin inhibitors cyclosporin A and FK506.
    Authors: Akool el-S, Doller A, Babelova A, Tsalastra W, Moreth K, Schaefer L, Pfeilschifter J, Eberhardt W
    J. Immunol., 2008;181(4):2831-45.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  28. Transforming growth factor-beta1 suppresses airway hyperresponsiveness in allergic airway disease.
    Authors: Alcorn JF, Rinaldi LM, Jaffe EF, van Loon M, Bates JH, Janssen-Heininger YM, Irvin CG
    Am. J. Respir. Crit. Care Med., 2007;176(10):974-82.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  29. Increase in transforming growth factor-beta in the brain during infection is related to fever, not depression of spontaneous motor activity.
    Authors: Matsumura S, Shibakusa T, Fujikawa T, Yamada H, Inoue K, Fushiki T
    Neuroscience, 2007;144(3):1133-40.
    Species: Rat
    Sample Type: CSF
    Application: Neut
  30. Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling.
    Authors: Shintani Y, Maeda M, Chaika N, Johnson KR, Wheelock MJ
    Am. J. Respir. Cell Mol. Biol., 2007;38(1):95-104.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  31. Local administration of interleukin-11 ameliorates intestinal radiation injury in rats.
    Authors: Boerma M, Wang J, Burnett AF, Santin AD, Roman JJ, Hauer-Jensen M
    Cancer Res., 2007;67(19):9501-6.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  32. Advanced glycation end products decrease mesangial cell MMP-7: a role in matrix accumulation in diabetic nephropathy?
    Authors: McLennan SV, Kelly DJ, Schache M, Waltham M, Dy V, Langham RG, Yue DK, Gilbert RE
    Kidney Int., 2007;72(4):481-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  33. Angiotensin II type 1 receptor blockade attenuates TGF-beta-induced failure of muscle regeneration in multiple myopathic states.
    Authors: Cohn RD, van Erp C, Habashi JP, Soleimani AA, Klein EC, Lisi MT, Gamradt M, ap Rhys CM, Holm TM, Loeys BL, Ramirez F, Judge DP, Ward CW, Dietz HC
    Nat. Med., 2007;13(2):204-10.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  34. Uptake of host cell transforming growth factor-beta by Trypanosoma cruzi amastigotes in cardiomyocytes: potential role in parasite cycle completion.
    Authors: Waghabi MC, Keramidas M, Bailly S, Degrave W, Mendonca-Lima L, Soeiro Mde N, Meirelles Mde N, Paciornik S, Araujo-Jorge TC, Feige JJ
    Am. J. Pathol., 2005;167(4):993-1003.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Electron Microscopy
  35. Nitric oxide induces TIMP-1 expression by activating the transforming growth factor beta-Smad signaling pathway.
    Authors: Akool el-S, Doller A, Muller R, Gutwein P, Xin C, Huwiler A, Pfeilschifter J, Eberhardt W
    J. Biol. Chem., 2005;280(47):39403-16.
    Species: Rat
    Sample Type: Whole Cells
    Application: Neut
  36. Thrombospondin plays a vital role in the immune privilege of the eye.
    Authors: Zamiri P, Masli S, Kitaichi N, Taylor AW, Streilein JW
    Invest. Ophthalmol. Vis. Sci., 2005;46(3):908-19.
    Species: Mink
    Sample Type: Whole Cells
    Application: Neut
  37. Neuronal TGF-beta1 mediates IL-9/mast cell interaction and exacerbates excitotoxicity in newborn mice.
    Authors: Mesples B, Fontaine RH, Lelievre V, Launay JM, Gressens P
    Neurobiol. Dis., 2005;18(1):193-205.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  38. Effect of weightbearing on bone formation during distraction osteogenesis.
    Authors: Leung KS, Cheung WH, Yeung HY, Lee KM, Fung KP
    Clin. Orthop. Relat. Res., 2004;0(419):251-7.
    Species: Goat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  39. Immunoneutralization of growth differentiation factor 9 reveals it partially accounts for mouse oocyte mitogenic activity.
    Authors: Gilchrist RB, Ritter LJ, Cranfield M, Jeffery LA, Amato F, Scott SJ, Myllymaa S, Kaivo-Oja N, Lankinen H, Mottershead DG, Groome NP, Ritvos O
    Biol. Reprod., 2004;71(3):732-9.
    Species: Bovine
    Sample Type: Whole Cells
    Application: Neut
  40. Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
    Authors: Torrealba JR, Katayama M, Fechner JH, Jankowska-Gan E, Kusaka S, Schultz JM, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ
    J. Immunol., 2004;172(9):5753-64.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Neut
  41. Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10.
    Authors: Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA
    J. Immunol., 2004;172(9):5213-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  42. Connective tissue growth factor and its correlation to other growth factors in experimental granulation tissue.
    Authors: Inkinen K, Wolff H, Lindroos P, Ahonen J
    Connect. Tissue Res., 2003;44(1):19-29.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  43. Estrogen inhibition of PTH-stimulated osteoclast formation and attachment in vitro: involvement of both PKA and PKC.
    Authors: Liu BY, Wu PW, Bringhurst FR, Wang JT
    Endocrinology, 2002;143(2):627-35.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
Expand to show all 43 Citations
Isotype Controls
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Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
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Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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ApplicationBlock/Neutralize
Sample TestedPeritoneal macrophages
SpeciesMouse

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