TGF-beta 1/1.2 Antibody

Catalog # Availability Size / Price Qty
AB-101-NA
TGF‑ beta 1 Inhibition ofIL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1/1.2 Antibody.
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Product Details
Citations (3)
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TGF-beta 1/1.2 Antibody Summary

Specificity
Detects TGF‑ beta 1/1.2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant amphibian TGF‑ beta 5 is observed and less than 2% cross-reactivity with recombinant human TGF‑ beta 3, recombinant chicken TGF‑ beta 3, and porcine TGF‑ beta 2 is observed.
Source
Polyclonal Chicken IgY
Purification
Ion exchange-purified from egg yolks
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human TGF‑ beta 1 (R&D Systems, Catalog # 240-B)
Ala279-Ser390
Accession # P01137
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human TGF-beta 1 (Catalog # 240-B)
Recombinant Human TGF-beta 1.2 (Catalog # 304-B3)
Neutralization
Measured by its ability to neutralize TGF‑ beta 1 inhibition of IL‑4-dependent proliferation in the HT‑2 mouse T cell line [Tsang, M. et al. (1995) Cytokine 7:389]. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 0.25 ng/mL Recombinant Human TGF‑ beta 1 and 7.5 ng/mL Recombinant Mouse IL‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization TGF‑ beta 1 Inhibition ofIL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1/1.2 Antibody. View Larger

TGF‑ beta 1 Inhibition of
IL‑4-dependent Cell Proliferation and Neutralization by TGF‑ beta 1/1.2 Antibody. Recombinant Human TGF‑ beta 1 (Catalog # 240-B) inhibits Recombinant Mouse IL‑4 (Catalog # 404-ML) induced proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL‑4 (7.5 ng/mL) activity elicited by Recombinant Human TGF‑ beta 1 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Chicken Anti-TGF‑ beta 1/1.2 Polyclonal Antibody (Catalog # AB-101-NA). The ND50 is typically 0.2-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TGF-beta 1/1.2

TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure. TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. Each TGF-beta isoform has some non-redundant functions; for TGF-beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF-beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N-terminal 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF- beta 1. Disulfide-linked homodimers of LAP and TGF-beta 1 remain non-covalently associated after secretion, forming the small latent TGF-beta 1 complex. Covalent linkage of LAP to one of three latent TGF-beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF-beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF-beta 1 shares 100% aa identity with pig, dog and cow TGF-beta 1, and 99% aa identity with mouse, rat and horse TGF-beta 1. It demonstrates cross-species activity. TGF-beta 1 signaling begins with high-affinity binding to a type II ser/thr kinase receptor termed TGF-beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF-beta RI (also called activin receptor-like kinase (ALK) -5), or alternatively, ALK‑1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF-beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF-beta in different contexts.

Long Name
Transforming Growth Factor beta 1 and 1.2
Entrez Gene IDs
7040 (Human); 21803 (Mouse); 59086 (Rat)
Alternate Names
TGFb1.2; TGF-beta 1/1.2

Product Datasheets

Citations for TGF-beta 1/1.2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Cancer associated fibroblasts regulate keratinocyte cell-cell adhesion via TGF-�-dependent pathways in genotype-specific oral cancer
    Authors: S S Prime
    Carcinogenesis, 2016;0(0):.
    Species: Human
    Sample Types: Conditioned Media
    Applications: Neut
  2. Cord blood mesenchymal stem cells suppress DC-T Cell proliferation via prostaglandin B2.
    Authors: van den Berk L, Jansen B, Snowden S, Siebers-Vermeulen K, Gilissen C, Kogler G, Figdor C, Wheelock C, Torensma R
    Stem Cells Dev, 2014;23(14):1582-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  3. Isoform specificity of commercially-available anti-TGF-beta antibodies.
    Authors: Mozes MM, Hodics T, Kopp JB
    J. Immunol. Methods, 1999;225(1):87-93.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: WB

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