TGF‑ beta 3 Biotinylated Antibody

Name: TGF‑ beta 3 Biotinylated Antibody
Brand: R&D Systems
SKU: BAM243
Price: 519 USD
Availability: InStock

R&D Systems | Catalog # BAM243

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Product Specifications
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Multi-Species

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # 20724

View all TGF-beta 3 Primary Antibodies »

Product Specifications

Immunogen

recombinant human (rh) TGF-beta 3 Ala301-Ser412 (Tyr340Phe), accession # P10600, mixed with rhTGF-beta 1, rhTGF-beta 2, recombinant canine TGF‑ beta 3, and recombinant amphibian TGF-beta 5

Specificity

Detects human TGF-beta 3 in Western blots and direct ELISAs. In Western blots and direct ELISAs, approximately 20% cross‑reactivity with TGF-beta 1.2 and TGF-beta 2 is observed, 5% cross-reactivity with TGF-beta 1 is observed and no cross-reactivity with recombinant human (rh) ART, recombinant mouse Artemin, recombinant drosophilia DPP, rhGDNF, rhLAP, rhLAT TGF-beta 1, rhLEFTY, recombinant rat MIS, rhNeurturin, recombinant amphibian TGF-beta 5, or rhTGF-alpha is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for TGF‑ beta 3 Biotinylated Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human TGF-beta 3 (Catalog # 243-B3) under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: TGF-beta 3

TGF-beta 1, -2 and -3 are a closely related group of proteins (70‑80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta 1, -2 and -3 are disulfide-linked homodimers.