TNF RII/TNFRSF1B Antibody (JM113-01)

Name: TNF RII/TNFRSF1B Antibody (JM113-01)
Brand: Novus Biologicals
SKU: NBP2-67218
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-67218

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JM113-01 expressed in HEK293

View all TNF RII/TNFRSF1B Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human TNF RII/TNFRSF1B aa 426-461 / 461. (SwissProt: P20333 Human; SwissProt: P25119 Mouse; SwissProt: Q80WY6 Rat)

Localization

Cell membrane, Membrane, Secreted.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TNF RII/TNFRSF1B Antibody (JM113-01)

Western Blot: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218]

Western Blot: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Western blot analysis of TNF RII/TNFRSF1B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 2: MCF-7 cell lysate Lane 2: Jurkat cell lysate Lane 2: Hela cell lysate

Immunocytochemistry/ Immunofluorescence: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218]

Immunocytochemistry/Immunofluorescence: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Staining TNF Receptor II in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218]

Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Analysis of paraffin-embedded mouse kidney tissue anti-TNF Receptor II antibody. Counter stained with hematoxylin.

Flow Cytometry: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218]

Flow Cytometry: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Analysis of HL-60 cells with TNF Receptor II antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218]

Immunocytochemistry/Immunofluorescence: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Staining TNF Receptor II in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/ Immunofluorescence: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218]

Immunocytochemistry/Immunofluorescence: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Staining TNF Receptor II in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218]

Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Analysis of paraffin-embedded human kidney tissue using anti-TNF Receptor II antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218]

Immunohistochemistry-Paraffin: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218] - Analysis of paraffin-embedded human uterus tissue using anti-TNF Receptor II antibody. Counter stained with hematoxylin.

Western Blot: TNF RII/TNFRSF1B Antibody (JM113-01) [NBP2-67218]

Western blot analysis on different lysates with NBP2-67218 at 1/2,000 dilution. Lane 1: THP-1 cell lysate (20 µg/Lane) Lane 2: Jurkat cell lysate (20 µg/Lane) Lane 3: K-562 cell lysate (20 µg/Lane) Lane 4: RAW264.7 cell lysate (20 µg/Lane) Lane 5: PC-12 cell lysate (20 µg/Lane) Lane 6: Mouse spleen tissue lysate (40 µg/Lane) Predicted band size: 48 kDa Observed band size: 60 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67218 at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

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Applications for TNF RII/TNFRSF1B Antibody (JM113-01)

Application

Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Frozen

1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TNF RII/TNFRSF1B

Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B

Gene Symbol

TNFRSF1B

Additional TNF RII/TNFRSF1B Products

Product Documents for TNF RII/TNFRSF1B Antibody (JM113-01)

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Certificate of Analysis

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Product Specific Notices for TNF RII/TNFRSF1B Antibody (JM113-01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.