TOR/mTOR [p Ser2448] Antibody - BSA Free

Novus Biologicals | Catalog # NB600-607

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TOR Primary Antibodies »

Product Specifications

Immunogen

TOR/TOR/mTOR [p Ser2448] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding a c-terminal region near Serine 2448 of human TOR/mTOR. (Uniprot: P42345)

Reactivity Notes

Reactivity against TOR/mTOR from other species has not been determined, however, reactivity with mouse is suggested based on protein sequence homologies

Modification

p Ser2448

Localization

Cell Membrane and Cytoplasmic

Specificity

Reactivity occurs with phosphorylated mTOR from human derived tissues and cells. Reactivity against mTOR from other species has not been determined, however, reactivity with mouse and rat is suggested based on protein sequence homologies.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for TOR/mTOR [p Ser2448] Antibody - BSA Free

Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Affinity Purified TOR/TOR/mTOR [p Ser2448] antibody is shown to detect a 250 kDa band (indicated) corresponding to phosphorylated human TOR/mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20 ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.
Immunohistochemistry: TOR/mTOR [p Ser2448] Antibody [NB600-607] - affinity purified TOR/TOR/mTOR [p Ser2448] antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney (40X). Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607]

Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607]

TOR-mTOR-[p-Ser2448]-Antibody-Western-Blot-NB600-607-img0006.jpg
Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607]

Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607]

Immunohistochemistry-Paraffin: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Brown arrow= IBa1 positive cells, Green arrow= mTOR positive cells, Black arrow= mTOR and IBA1 positive cells. IHC-P Image submitted by a verified customer review.
ELISA: TOR/mTOR [p Ser2448] Antibody [NB600-607] - ELISA Results of TOR/TOR/mTOR [p Ser2448] antibody tested against BSA-conjugated non-phospho [purple line] and phospho [blue line] forms of immunizing peptide. Each well was coated in duplicate with either 0.1ug of conjugate. The working dilution is 1:37,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP conjugate stabilizer, Goat Anti-Rabbit HRP conjugated and TMB substrate.
TOR/mTOR [p Ser2448] Antibody

TOR/mTOR [p Ser2448] Antibody

affinity purified anti-mTOR pS 2448 antibody was used at 5 ug/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate staining of proximal convoluted tubules of the kidney (40X). Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.
TOR/mTOR [p Ser2448] Antibody

Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] -

Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Analysis of mTOR phosphorylation during microglial activation(A) Whole-cell lysates were prepared from microglial cells incubated for 2 h as indicated. Equal amounts of proteins were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR), upper gel. The same blots were subsequently probed for beta -actin, lower gel. (B) Quantitation of densitometry where p-mTOR values are reflected relative to those for beta -actin. Data are expressed as means±S.E.M. of n=1 replicate for each group, each assayed in triplicates. Representative of two different experiments. Data were analyzed by one-way ANOVA followed by the Bonferroni's post hoc test. ***P<0.001 versus Control, °P<0.05 versus C-CM. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/24689533), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
TOR/mTOR [p Ser2448] Antibody

Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] -

Western Blot: TOR/mTOR [p Ser2448] Antibody [NB600-607] - Analysis of mammalian target of rapamycin (mTOR) phosphorylation during microglial activation. Whole-cell lysates were prepared from microglial cells activated by control-conditioned medium (C-CM) (A) or CM from glioma cells activated with lipopolysaccharide (LPS)/IFN gamma (LI-CM) (B). Equal amounts of proteins were analyzed by western blot for phosphorylated mTOR kinase (p-mTOR), upper gel & were subsequently probed for beta -actin, lower gel. Quantitation of densitometry where p-mTOR values are reflected relative to those for beta -actin. Data are expressed as means ± standard error of the mean of n = 1 replicate for each group, each assayed in triplicate: representative of two different experiments. Data were analyzed by one-way analysis of variance followed by Bonferroni post hoc test. ***P <0.001 versus control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/25051975), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TOR/mTOR [p Ser2448] Antibody - BSA Free

Application
Recommended Usage

ELISA

1:10000-1:100000

Immunohistochemistry

5.0 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in immunohistochemistry, ELISA and western blotting. Western blotting shows reactivity specific for phospho mTOR detecting a band at approximately 250 kDa. Reactivity in other immunoassays is unknown.

Reviewed Applications

Read 2 reviews rated 4 using NB600-607 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: TOR

Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the S. cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.

Long Name

Mammalian Target of Rapamycin

Alternate Names

FRAP1, FRAP2, MTOR, RAFT1

Entrez Gene IDs

2475 (Human)

Gene Symbol

MTOR

UniProt

P42345

Additional TOR Products

Product Documents for TOR/mTOR [p Ser2448] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TOR/mTOR [p Ser2448] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TOR/mTOR [p Ser2448] Antibody - BSA Free

Customer Reviews for TOR/mTOR [p Ser2448] Antibody - BSA Free (2)

4 out of 5
2 Customer Ratings
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • TOR/mTOR [p Ser2448] Antibody
    Name: Lucia Lisi
    Application: Western Blot
    Sample Tested: Human microglia cell lysate
    Species: Human
    Verified Customer | Posted 01/17/2020
    Chiavari M, Ciotti GMP, Navarra P, Lisi L. Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line. Brain Sci. 2019;9(5):111. Published 2019 May 15. doi:10.3390/brainsci9050111
    TOR/mTOR [p Ser2448] Antibody - BSA Free NB600-607
  • TOR/mTOR [p Ser2448] Antibody
    Name: Lucia Lisi
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Human Brain Tissue
    Species: Human
    Verified Customer | Posted 01/17/2020
    Brown arrow= IBa1 positive cells Green arrow= mTOR positive cells Black arrow= mTOR and IBA1 positive cells
    TOR/mTOR [p Ser2448] Antibody - BSA Free NB600-607

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